Literature DB >> 6998960

Comparison of the polypeptide composition of Escherichia coli outer membranes prepared by two methods.

I Chopra, S W Shales.   

Abstract

Escherichia coli outer membranes were prepared by centrifugation to equilibrium in sucrose gradients and then treated with Sarkosyl in the presence of ethylenediaminetetraacetate. The polypeptide profiles of the two outer membrane preparations were compared by two-dimensional polyacrylamide gel electrophoresis. The patterns obtained were not identical, and Sarkosyl removed several minor proteins from the outer membrane.

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Year:  1980        PMID: 6998960      PMCID: PMC294674          DOI: 10.1128/jb.144.1.425-427.1980

Source DB:  PubMed          Journal:  J Bacteriol        ISSN: 0021-9193            Impact factor:   3.490


  14 in total

1.  High resolution two-dimensional electrophoresis of proteins.

Authors:  P H O'Farrell
Journal:  J Biol Chem       Date:  1975-05-25       Impact factor: 5.157

2.  Visualization of proteins after isoelectric focusing during two-dimensional gel electrophoresis.

Authors:  H Jäckle
Journal:  Anal Biochem       Date:  1979-09-15       Impact factor: 3.365

3.  Diffusion of tetracycline across the outer membrane of Escherichia coli K-12: involvement of protein Ia.

Authors:  I Chopra; S J Eccles
Journal:  Biochem Biophys Res Commun       Date:  1978-07-28       Impact factor: 3.575

4.  Binding of bicyclomycin to inner membrane proteins of E. coli.

Authors:  A Someya; M Iseki; N Tanaka
Journal:  J Antibiot (Tokyo)       Date:  1978-07       Impact factor: 2.649

5.  Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane.

Authors:  M J Osborn; J E Gander; E Parisi; J Carson
Journal:  J Biol Chem       Date:  1972-06-25       Impact factor: 5.157

6.  R factor proteins synthesized in Escherichia coli minicells: membrane-associated R factor proteins.

Authors:  S B Levy; L McMurry; E Palmer
Journal:  J Bacteriol       Date:  1974-12       Impact factor: 3.490

Review 7.  The outer membrane of Gram-negative bacteria.

Authors:  H Nikaido; T Nakae
Journal:  Adv Microb Physiol       Date:  1979       Impact factor: 3.517

8.  Quantitation of proteins solubilized in sodium dodecyl sulfate-mercaptoethanol-Tris electrophoresis buffer.

Authors:  Z Zaman; R L Verwilghen
Journal:  Anal Biochem       Date:  1979-11-15       Impact factor: 3.365

9.  Outer membrane of Salmonella typhimurium: chemical analysis and freeze-fracture studies with lipopolysaccharide mutants.

Authors:  J Smit; Y Kamio; H Nikaido
Journal:  J Bacteriol       Date:  1975-11       Impact factor: 3.490

10.  Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium-lauryl sarcosinate.

Authors:  C Filip; G Fletcher; J L Wulff; C F Earhart
Journal:  J Bacteriol       Date:  1973-09       Impact factor: 3.490

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  18 in total

1.  Characterization of Esterase A, a Pseudomonas stutzeri A15 Autotransporter.

Authors:  Toon Nicolay; Ken Devleeschouwer; Jos Vanderleyden; Stijn Spaepen
Journal:  Appl Environ Microbiol       Date:  2012-02-03       Impact factor: 4.792

2.  Unequal distribution of penicillin-binding proteins among inner membrane vesicles of Escherichia coli.

Authors:  G H Jacoby; K D Young
Journal:  J Bacteriol       Date:  1988-08       Impact factor: 3.490

3.  Outer membrane protein profiles of Haemophilus pleuropneumoniae.

Authors:  V J Rapp; R S Munson; R F Ross
Journal:  Infect Immun       Date:  1986-05       Impact factor: 3.441

4.  Specific location of penicillin-binding proteins within the cell envelope of Escherichia coli.

Authors:  J A Barbas; J Díaz; A Rodríguez-Tébar; D Vázquez
Journal:  J Bacteriol       Date:  1986-01       Impact factor: 3.490

5.  Location of some proteins involved in peptidoglycan synthesis and cell division in the inner and outer membranes of Escherichia coli.

Authors:  A Rodríguez-Tébar; J A Barbas; D Vázquez
Journal:  J Bacteriol       Date:  1985-01       Impact factor: 3.490

6.  Absence of significant membrane localization of the proteins coded by the ilvGEDAC genes of Escherichia coli K-12.

Authors:  J E Gray; D H Calhoun
Journal:  J Bacteriol       Date:  1982-07       Impact factor: 3.490

7.  A novel protein, TtpC, is a required component of the TonB2 complex for specific iron transport in the pathogens Vibrio anguillarum and Vibrio cholerae.

Authors:  Michiel Stork; Ben R Otto; Jorge H Crosa
Journal:  J Bacteriol       Date:  2006-12-22       Impact factor: 3.490

8.  Role for Escherichia coli YidD in membrane protein insertion.

Authors:  Zhong Yu; Mariëlle Lavèn; Mirjam Klepsch; Jan-Willem de Gier; Wilbert Bitter; Peter van Ulsen; Joen Luirink
Journal:  J Bacteriol       Date:  2011-07-29       Impact factor: 3.490

9.  Modification, processing, and subcellular localization in Escherichia coli of the pCloDF13-encoded bacteriocin release protein fused to the mature portion of beta-lactamase.

Authors:  J Luirink; T Watanabe; H C Wu; F Stegehuis; F K de Graaf; B Oudega
Journal:  J Bacteriol       Date:  1987-05       Impact factor: 3.490

10.  Release of outer membrane fragments by exponentially growing Brucella melitensis cells.

Authors:  C Gamazo; I Moriyón
Journal:  Infect Immun       Date:  1987-03       Impact factor: 3.441

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