Literature DB >> 6997991

Directed deletion of a yeast transfer RNA intervening sequence.

R B Wallace, P F Johnson, S Tanaka, M Schöld, K Itakura, J Abelson.   

Abstract

Many eukaryotic genes contain intevening sequences, segments of DNA that interrupt the continuity of the gene. They are removed from RNA transcripts of the gene by a process known as splicing. The intervening sequence in a yeast tyrosine transfer RNA (tRNA Tyr) suppressor gene was deleted in order to test its role in the expression of the gene. The altered gene and its parent were introduced into yeast by transformation. Both genes exhibited suppressor function, showing that the intervening sequence is not absolutely essential for the expression of this gene.

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Year:  1980        PMID： 6997991     DOI： 10.1126/science.6997991

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  40 in total

1.  Preferential binding of yeast tRNA ligase to pre-tRNA substrates.

Authors:  B L Apostol; C L Greer
Journal:  Nucleic Acids Res       Date:  1991-04-25       Impact factor: 16.971

2.  Gating of a voltage-dependent channel (colicin E1) in planar lipid bilayers: translocation of regions outside the channel-forming domain.

Authors:  L Raymond; S L Slatin; A Finkelstein; Q R Liu; C Levinthal
Journal:  J Membr Biol       Date:  1986       Impact factor: 1.843

3.  All human tRNATyr genes contain introns as a prerequisite for pseudouridine biosynthesis in the anticodon.

Authors:  H van Tol; H Beier
Journal:  Nucleic Acids Res       Date:  1988-03-25       Impact factor: 16.971

4.  Role of positive charge on the amino-terminal region of the signal peptide in protein secretion across the membrane.

Authors:  S Inouye; X Soberon; T Franceschini; K Nakamura; K Itakura; M Inouye
Journal:  Proc Natl Acad Sci U S A       Date:  1982-06       Impact factor: 11.205

5.  Sealing of gaps in duplex DNA by T4 DNA ligase.

Authors:  S V Nilsson; G Magnusson
Journal:  Nucleic Acids Res       Date:  1982-03-11       Impact factor: 16.971

6.  Assembly of a tRNA splicing complex: evidence for concerted excision and joining steps in splicing in vitro.

Authors:  C L Greer
Journal:  Mol Cell Biol       Date:  1986-02       Impact factor: 4.272

7.  Introduction of restriction enzyme sites in protein-coding DNA sequences by site-specific mutagenesis not affecting the amino acid sequence: a computer program.

Authors:  R Arentzen; W C Ripka
Journal:  Nucleic Acids Res       Date:  1984-01-11       Impact factor: 16.971

8.  Kluyveromyces lactis maintains Saccharomyces cerevisiae intron-encoded splicing signals.

Authors:  J O Deshler; G P Larson; J J Rossi
Journal:  Mol Cell Biol       Date:  1989-05       Impact factor: 4.272

9.  In vitro generation of specific deletions in DNA cloned in M13 vectors using synthetic oligodeoxyribonucleotides: mutants in the 5'-flanking region of the yeast alcohol dehydrogenase II gene.

Authors:  V L Chan; M Smith
Journal:  Nucleic Acids Res       Date:  1984-03-12       Impact factor: 16.971

10.  Rapid synthesis of long-chain deoxyribooligonucleotides by the N-methylimidazolide phosphotriester method.

Authors:  V A Efimov; A A Buryakova; S V Reverdatto; O G Chakhmakhcheva
Journal:  Nucleic Acids Res       Date:  1983-12-10       Impact factor: 16.971
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