Literature DB >> 6994718

Effect of ethanol on glutathione concentration in isolated hepatocytes.

J Viña, J M Estrela, C Guerri, F J Romero.   

Abstract

1. Ethanol induces a decrease in GSH (reduced glutathione) concentration is isolated hepatocytes. Maximal effects appear at 20 mM-ethanol. The concentration-dependence of this decrease is paralleled by the concentration-dependence of the activity of alcohol dehydrogenase. 2. Pyrazole, a specific inhibitor of alcohol dehydrogenase, prevents the ethanol-induced GSH depletion. 3. Acetaldehyde, above 0.05 mM, also promotes a decrease in GSH concentration in hepatocytes. 4. Disulfiram (0.05 mM), an inhibitor of aldehyde dehydrogenase, potentiates the fall in GSH concentration caused by acetaldehyde. 5. The findings support the hypothesis that acetaldehyde is responsible for the depletion of GSH induced by ethanol. 6. Methionine prevents the effect of alcohol or acetaldehyde on GSH concentration in hepatocytes.

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Year:  1980        PMID： 6994718      PMCID： PMC1161900          DOI： 10.1042/bj1880549

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  14 in total

1.  On the oxidation of succinate by parenchymal cells isolated from rat liver.

Authors:  J P Mapes; R A Harris
Journal:  FEBS Lett       Date:  1975-03-01       Impact factor: 4.124

2.  Some properties of mitochondrial glutathione.

Authors:  P C Jocelyn
Journal:  Biochim Biophys Acta       Date:  1975-09-08

3.  Mechanism of the protective action of cysteine and penicillamine against acetaldehyde-induced mitochondrial injury.

Authors:  A I Cederbaum; E Rubin
Journal:  Biochem Pharmacol       Date:  1976-10-01       Impact factor: 5.858

4.  The role of methionine in glutathione biosynthesis by isolated hepatocytes.

Authors:  D J Reed; S Orrenius
Journal:  Biochem Biophys Res Commun       Date:  1977-08-22       Impact factor: 3.575

5.  Brain aldehyde dehydrogenase. Localization, purification, and properties.

Authors:  V G Erwin; R A Deitrich
Journal:  J Biol Chem       Date:  1966-08-10       Impact factor: 5.157

6.  Studies on the regulation of glutathione level in rat liver.

Authors:  N Tateishi; T Higashi; S Shinya; A Naruse; Y Sakamoto
Journal:  J Biochem       Date:  1974-01       Impact factor: 3.387

7.  Inhibition of hepatic gluconeogenesis by ethanol.

Authors:  H A Krebs; R A Freedland; R Hems; M Stubbs
Journal:  Biochem J       Date:  1969-03       Impact factor: 3.857

8.  The kinetics and mechanism of liver alcohol dehydrogenase with primary and secondary alcohols as substrates.

Authors:  K Dalziel; F M Dickinson
Journal:  Biochem J       Date:  1966-07       Impact factor: 3.857

9.  The redox state of free nicotinamide-adenine dinucleotide in the cytoplasm and mitochondria of rat liver.

Authors:  D H Williamson; P Lund; H A Krebs
Journal:  Biochem J       Date:  1967-05       Impact factor: 3.857

10.  High-yield preparation of isolated rat liver parenchymal cells: a biochemical and fine structural study.

Authors:  M N Berry; D S Friend
Journal:  J Cell Biol       Date:  1969-12       Impact factor: 10.539
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  18 in total

1.  Zinc, ethanol, and lipid peroxidation in adult and fetal rats.

Authors:  I E Dreosti; E J Partick
Journal:  Biol Trace Elem Res       Date:  1987-12       Impact factor: 3.738

2.  Ethanol-induced gastrointestinal damage. Influence of endogenous antioxidant components and gender.

Authors:  M H Moghadasian; D V Godin
Journal:  Dig Dis Sci       Date:  1996-04       Impact factor: 3.199

3.  S-adenosyl-methionine decreases ethanol-induced apoptosis in primary hepatocyte cultures by a c-Jun N-terminal kinase activity-independent mechanism.

Authors:  Maria del Pilar Cabrales-Romero; Lucrecia Márquez-Rosado; Samia Fattel-Fazenda; Cristina Trejo-Solís; Evelia Arce-Popoca; Leticia Alemán-Lazarini; Saúl Villa-Treviño
Journal:  World J Gastroenterol       Date:  2006-03-28       Impact factor: 5.742

4.  Glutathione conjugation of chlorobenzylidene malononitriles in vitro and the biotransformation to mercapturic acids in rats.

Authors:  E C Rietveld; M M Hendrikx; F Seutter-Berlage
Journal:  Arch Toxicol       Date:  1986-12       Impact factor: 5.153

5.  Conjugation of acetaldehyde with cysteinylglycine, the first metabolite in glutathione breakdown by gamma-glutamyltranspeptidase.

Authors:  Y Kera; T Kiriyama; S Komura
Journal:  Agents Actions       Date:  1985-10

6.  Increased loss and decreased synthesis of hepatic glutathione after acute ethanol administration. Turnover studies.

Authors:  H Speisky; A MacDonald; G Giles; H Orrego; Y Israel
Journal:  Biochem J       Date:  1985-02-01       Impact factor: 3.857

7.  Effect of acute and chronic ethanol ingestion on the content of reduced glutathione of various tissues of the rat.

Authors:  V Fernández; L A Videla
Journal:  Experientia       Date:  1981-04-15

8.  Effect of chronic ethanol feeding on rat hepatocytic glutathione. Compartmentation, efflux, and response to incubation with ethanol.

Authors:  J C Fernandez-Checa; M Ookhtens; N Kaplowitz
Journal:  J Clin Invest       Date:  1987-07       Impact factor: 14.808

9.  Behavioral and Biochemical Effects of N-Acetylcysteine in Zebrafish Acutely Exposed to Ethanol.

Authors:  Ricieri Mocelin; Matheus Marcon; Simone D'ambros; Ana P Herrmann; Alex Sander da Rosa Araujo; Angelo Piato
Journal:  Neurochem Res       Date:  2017-12-01       Impact factor: 3.996

10.  Ethanol-induced upregulation of 10-formyltetrahydrofolate dehydrogenase helps relieve ethanol-induced oxidative stress.

Authors:  Tsun-Hsien Hsiao; Chia-Jen Lin; Yi-Shao Chung; Gang-Hui Lee; Tseng-Ting Kao; Wen-Ni Chang; Bing-Hung Chen; Jan-Jong Hung; Tzu-Fun Fu
Journal:  Mol Cell Biol       Date:  2013-11-25       Impact factor: 4.272
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