Literature DB >> 6993338

Detection of laser--UV microirradiation-induced DNA photolesions by immunofluorescent staining.

C Cremer, T Cremer, M Fukuda, K Nakanishi.   

Abstract

A low-power laser-UV microbeam of wavelength 257 nm was used for microirradiation of a small part of the nucleus of Chinese hamster cells. Following fixation in interphase or in the subsequent metaphase indirect immunofluorescent staining was performed with antiserum to photoproducts of DNA treated with far UV light. The results show that antibodies specific for UV-irradiated DNA can be used for a direct detection of laser-UV microirradiation-induced DNA photolesions. The potential usefulness of this method for investigation of the spatial arrangement of chromosomes in the interphase nucleus is discussed.

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Year:  1980        PMID： 6993338     DOI： 10.1007/bf00279058

Source DB:  PubMed          Journal:  Hum Genet        ISSN： 0340-6717            Impact factor:   4.132


  17 in total

1.  Effects of laser uv-microirradiation (lambda = 2573 A) on proliferation of Chinese hamster cells.

Authors:  C Cremer; T Cremer; C Zorn; L Schoeller
Journal:  Radiat Res       Date:  1976-04       Impact factor: 2.841

2.  Pattern of ultra-violet-light-induced repair in metaphase and interphase chromosomes.

Authors:  R T Johnson; K Sperling
Journal:  Int J Radiat Biol Relat Stud Phys Chem Med       Date:  1978-12

3.  Immunological demonstration of the disappearance of pyrimidine dimers from nuclei of cultured human cells.

Authors:  C J Lucas
Journal:  Exp Cell Res       Date:  1972-10       Impact factor: 3.905

4.  The internal order of the interphase nucleus.

Authors:  F Vogel; T M Schroeder
Journal:  Humangenetik       Date:  1974

5.  DNA-degradation products from mammalian cells irradiated with ultra-violet light.

Authors:  J E Cleaver; J E Trosko
Journal:  Int J Radiat Biol Relat Stud Phys Chem Med       Date:  1969-07

6.  The rationale for an ordered arrangement of chromatin in the interphase nucleus.

Authors:  D E Comings
Journal:  Am J Hum Genet       Date:  1968-09       Impact factor: 11.025

7.  Effects of ultraviolet micro-irradiation on different parts of the cell. II. Cytological observations and unscheduled DNA synthesis after partial nuclear irradiation.

Authors:  G Moreno
Journal:  Exp Cell Res       Date:  1971-03       Impact factor: 3.905

8.  Unscheduled DNA synthesis after partial UV irradiation of the cell nucleus. Distribution in interphase and metaphase.

Authors:  C Zorn; C Cremer; T Cremer; J Zimmer
Journal:  Exp Cell Res       Date:  1979-11       Impact factor: 3.905

9.  Antibodies to photoproducts of deoxyribonucleic acids irradiated with ultraviolet light.

Authors:  L Levine; E Seaman; E Hammerschlag; H Van Vunakis
Journal:  Science       Date:  1966-09-30       Impact factor: 47.728

10.  Differences in removal of acetylaminofluorene and pyrimidine dimers from the DNA of cultured mammalian cells.

Authors:  D E Amacher; J A Elliott; M W Lieberman
Journal:  Proc Natl Acad Sci U S A       Date:  1977-04       Impact factor: 11.205
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  13 in total

1.  DNA multiphoton absorption generates localized damage for studying repair dynamics in live cells.

Authors:  Matthew K Daddysman; Christopher J Fecko
Journal:  Biophys J       Date:  2011-11-01       Impact factor: 4.033

Review 2.  DNA damage response.

Authors:  Giuseppina Giglia-Mari; Angelika Zotter; Wim Vermeulen
Journal:  Cold Spring Harb Perspect Biol       Date:  2011-01-01       Impact factor: 10.005

3.  Recollections of a scientific journey published in human genetics: from chromosome territories to interphase cytogenetics and comparative genome hybridization.

Authors:  Thomas Cremer; Christoph Cremer; Peter Lichter
Journal:  Hum Genet       Date:  2014-02-07       Impact factor: 4.132

4.  Rabl's model of the interphase chromosome arrangement tested in Chinese hamster cells by premature chromosome condensation and laser-UV-microbeam experiments.

Authors:  T Cremer; C Cremer; H Baumann; E K Luedtke; K Sperling; V Teuber; C Zorn
Journal:  Hum Genet       Date:  1982       Impact factor: 4.132

5.  Analysis of chromosome positions in the interphase nucleus of Chinese hamster cells by laser-UV-microirradiation experiments.

Authors:  T Cremer; C Cremer; T Schneider; H Baumann; L Hens; M Kirsch-Volders
Journal:  Hum Genet       Date:  1982       Impact factor: 4.132

6.  A novel and simple micro-irradiation technique for creating localized DNA double-strand breaks.

Authors:  Keiji Suzuki; Motohiro Yamauchi; Yasuyoshi Oka; Masatoshi Suzuki; Shunichi Yamashita
Journal:  Nucleic Acids Res       Date:  2010-04-12       Impact factor: 16.971

7.  Monitoring DNA breaks in optically highlighted chromatin in living cells by laser scanning confocal microscopy.

Authors:  Michael J Kruhlak; Arkady Celeste; André Nussenzweig
Journal:  Methods Mol Biol       Date:  2009

Review 8.  DNA in motion during double-strand break repair.

Authors:  Judith Miné-Hattab; Rodney Rothstein
Journal:  Trends Cell Biol       Date:  2013-07-15       Impact factor: 20.808

9.  Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells.

Authors:  Nathaniel W Holton; Joel F Andrews; Natalie R Gassman
Journal:  J Vis Exp       Date:  2017-09-05       Impact factor: 1.355

10.  Specific local induction of DNA strand breaks by infrared multi-photon absorption.

Authors:  D Träutlein; M Deibler; A Leitenstorfer; E Ferrando-May
Journal:  Nucleic Acids Res       Date:  2009-11-11       Impact factor: 16.971
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