Literature DB >> 6988325

A simple reliable procedure for obtaining metaphases from human leukemic bone-marrow aspirates suitable for Giemsa banding.

A K Srivastava, R D Smith.   

Abstract

Short incubation of heparinized human leukemic bone-marrow cells in phosphate buffered saline containing colcemid and overnight chilling of fixed cells yields metaphases with elongated and well-spread chromosomes. This technique enables us to do trypsin-Giemsa banding of chromosomes obtained from leukemic marrow cells otherwise difficult to band.

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Year:  1980        PMID： 6988325     DOI： 10.1007/BF02831501

Source DB:  PubMed          Journal:  In Vitro        ISSN： 0073-5655


  4 in total

1.  Bone-marrow chromosomes in healthy subjects.

Authors:  S Knuutila; O Simell; P Lipponen; I Saarinen
Journal:  Hereditas       Date:  1976       Impact factor: 3.271

2.  An improved technique of preparing bone-marrow specimens for cytogenetic analysis.

Authors:  Y Shiloh; M M Cohen
Journal:  In Vitro       Date:  1978-06

3.  A rapid banding technique for human chromosomes.

Authors:  M Seabright
Journal:  Lancet       Date:  1971-10-30       Impact factor: 79.321

4.  Identification of chromosome anomalies in the blastic phase of chronic myelocytic leukemia (CML) by Giemsa- and quinacrine-banding techniques.

Authors:  D K Hossfeld
Journal:  Humangenetik       Date:  1974-07-15
  4 in total

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