Literature DB >> 355116

An improved technique of preparing bone-marrow specimens for cytogenetic analysis.

Y Shiloh, M M Cohen.   

Abstract

Sixty-six bone-marrow specimens, derived from patients with hematological and nonhematological disorders, were processed for cytogenetic analysis. Modifications of various parameters of the standard (direct) culture procedure were investigated and the quality of the preparations determined. As a result of these experiments, an improved culture technique was developed that yielded significantly better quality chromosome preparations. This method is based on a short incubation (25-min) of the bone-marrow specimen, immediately following aspiration, in a solution containing both hypotonic KCl and colcemid and completely omits the use of tissue culture medium.

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Year:  1978        PMID： 355116     DOI： 10.1007/bf02616092

Source DB:  PubMed          Journal:  In Vitro        ISSN： 0073-5655


  7 in total

1.  The chromosome constitution of human marrow in various developmental and blood disorders.

Authors:  A A SANDBERG; G F KOEPF; L H CROSSWHITE; T S HAUSCHKA
Journal:  Am J Hum Genet       Date:  1960-09       Impact factor: 11.025

2.  G-banding and chromosome structure.

Authors:  J J Yunis; O Sanchez
Journal:  Chromosoma       Date:  1973       Impact factor: 4.316

3.  Cytogenetic evidence for splenic origin of blastic transformation in chronic myeloid leukaemia.

Authors:  F Mitelman; L Brandt; P G Nilsson
Journal:  Scand J Haematol       Date:  1974

4.  The chronology of the mitotic cycle of human granulocytopoietic cells. Phase contrast studies on living cells in vitro.

Authors:  E G Rondanelli; E Magliulo; A Giraldi; F P Carcò
Journal:  Blood       Date:  1967-11       Impact factor: 22.113

5.  [Action of colchicine and of spreading on the width of the chromatid fiber].

Authors:  C Gilly; C Mouriquand; E Jeunet
Journal:  Ann Genet       Date:  1976-06

6.  Identification of chromosome anomalies in the blastic phase of chronic myelocytic leukemia (CML) by Giemsa- and quinacrine-banding techniques.

Authors:  D K Hossfeld
Journal:  Humangenetik       Date:  1974-07-15

7.  Chromosomes and causation of human cancer and leukemia. XVI. Banding studies of chronic myelocytic leukemia, including five unusual Ph11 translocations.

Authors:  I Hayata; M Sakurai; S Kakati; A A Sandberg
Journal:  Cancer       Date:  1975-10       Impact factor: 6.860
  7 in total
  5 in total

1.  Chromosome analysis of 30 cases of non-Hodgkin's lymphoma.

Authors:  G Chenevix-Trench; J A Brown; G B Tyler; F G Behm
Journal:  Med Oncol Tumor Pharmacother       Date:  1988

2.  A simple reliable procedure for obtaining metaphases from human leukemic bone-marrow aspirates suitable for Giemsa banding.

Authors:  A K Srivastava; R D Smith
Journal:  In Vitro       Date:  1980-02

3.  Cytogenetic analysis of leukaemic colonies from acute and chronic myelogenous leukaemia.

Authors:  J M Trent; J R Davis; B G Durie
Journal:  Br J Cancer       Date:  1983-01       Impact factor: 7.640

4.  Human tumour karyology: marked analytic improvement by short-term agar culture.

Authors:  J M Trent; S E Salmon
Journal:  Br J Cancer       Date:  1980-06       Impact factor: 7.640

5.  The complex translocation (9;14;14) involving IGH and CEBPE genes suggests a new subgroup in B-lineage acute lymphoblastic leukemia.

Authors:  Rachid Zerrouki; Traki Benhassine; Mustapha Bensaada; Patricia Lauzon; Anissa Trabzi
Journal:  Genet Mol Biol       Date:  2016-03       Impact factor: 1.771
  5 in total

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