Literature DB >> 6987533

Synthesis in E. coli of a polypeptide with human leukocyte interferon activity.

S Nagata, H Taira, A Hall, L Johnsrud, M Streuli, J Ecsödi, W Boll, K Cantell, C Weissmann.   

Abstract

Double-stranded cDNA prepared from the 12S fraction of poly(A) RNA from interferon (IF)-producing human leukocytes was cloned in Escherichia coli using the pBR322 vector. One of the resulting clones had a 910-base pair insert which could hybridise to IF mRNA and was responsible for the production of a polypeptide with biological IF activity. Up to 10,000 units IF activity per g of cells was obtained from some clones.

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Year:  1980        PMID： 6987533     DOI： 10.1038/284316a0

Source DB:  PubMed          Journal:  Nature        ISSN： 0028-0836            Impact factor:   49.962


  103 in total

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5.  A novel class of human type I interferons.

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6.  Human genomic library screened with 17-base oligonucleotide probes yields a novel interferon gene.

Authors:  R M Torczynski; M Fuke; A P Bollon
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7.  Differential expression of human interferon genes.

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8.  A phase I toxicity study of human rDNA interferon in patients with solid tumours.

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9.  Structure and expression in Escherichia coli of a cloned rat interferon-alpha gene.

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10.  Inducible expression of amplified human beta interferon genes in CHO cells.

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