Immunoperoxidase identification of intracellular immunoglobulins from cell smears.

Immunoperoxidase identification of intracellular immunoglobulins from cell smears. Am J Clin Pathol 73: 248--249, 1980. Optimal fixation procedures for intracellular immunoglobulins (Ig) were defined by using cytocentrifuged or conventional cell smears of pokeweed-mitogen-stimulated human lymphocytes. The Ig was detected by the unlabeled-antibody-enzyme technic. The results indicate that superior preservation of Ig antigenicity combined with good morphologic integrity is obtained with 2--4 min fixation in Baker's formol-calcium at 20 C with subsequent wash in distilled water. This technic is currently used for the detection of Ig isotypes from bone marrow aspirates and from B cell mitogen-stimulated cell cultures.