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Literature DB >> 6983072

T-cell cloning to detect the mutant 6-thioguanine-resistant lymphocytes present in human peripheral blood.

R J Albertini, K L Castle, W R Borcherding.

Abstract

Rare thioguanine-resistant T lymphocytes, present in vivo in human peripheral blood, were isolated and grown in vitro as thioguanine-resistant cultured T cells. The conditions for their selection in vitro were such that thioguanine resistance had to have arisen in vivo. The mutant cells bore T-cell surface markers, maintained their thioguanine resistance in vitro in the presence or absence of selection, and were deficient in hypoxanthine-guanine phosphoribosyltransferase activity.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 1982 PMID： 6983072 PMCID： PMC347179 DOI： 10.1073/pnas.79.21.6617

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

19 in total

1. Plateletpheresis using the haemonetics model 30 cell separator.

Authors: J Nusbacher; M L Scher; J L MacPherson
Journal: Vox Sang Date: 1977-07 Impact factor: 2.144

Review 2. Resistance of cultured human fibroblasts and other cells to purine and pyrimidine analogues in relation to mutagenesis detection.

Authors: R DeMars
Journal: Mutat Res Date: 1974-09 Impact factor: 2.433

3. Variability of the time at which PHA-stimulated lymphocytes initiate DNA synthesis.

Authors: L Sörén
Journal: Exp Cell Res Date: 1973-03-30 Impact factor: 3.905

4. The spontaneous azaguanine-resistant mutants of diploid human fibroblasts.

Authors: R DeMars; K R Held
Journal: Humangenetik Date: 1972

5. Precipitation of radioactively labeled samples: a semi-automatic multiple-sample processor.

Authors: R J Hartzman; M L Bach; F H Bach; G B Thurman; K W Sell
Journal: Cell Immunol Date: 1972-06 Impact factor: 4.868

6. Genetic studies of HG- PRT deficiency and the Lesch-Nyhan syndrome with cultured human cells.

Authors: R DeMars
Journal: Fed Proc Date: 1971 May-Jun

Review 7. Selective systems in somatic cell genetics.

Authors: E H Chu; S S Powell
Journal: Adv Hum Genet Date: 1976

8. Enzyme defect associated with a sex-linked human neurological disorder and excessive purine synthesis.

Authors: J E Seegmiller; F M Rosenbloom; W N Kelley
Journal: Science Date: 1967-03-31 Impact factor: 47.728

9. Phenotypic lag and mutation to 6-thioguanine resistance in diploid human lymphoblasts.

Authors: W G Thilly; J G Deluca; H Hoppe; B W Penman
Journal: Mutat Res Date: 1978-04 Impact factor: 2.433

10. Enumeration of 6-thioguanine-resistant peripheral blood lymphocytes in man as a potential test for somatic cell mutations arising in vivo.

Authors: G H Strauss; R J Albertini
Journal: Mutat Res Date: 1979-07 Impact factor: 2.433

46 in total

10. Homologies between T cell receptor junctional sequences unique to multiple sclerosis and T cells mediating experimental allergic encephalomyelitis.

Authors: M Allegretta; R J Albertini; M D Howell; L R Smith; R Martin; H F McFarland; S Sriram; S Brostoff; L Steinman
Journal: J Clin Invest Date: 1994-07 Impact factor: 14.808

T-cell cloning to detect the mutant 6-thioguanine-resistant lymphocytes present in human peripheral blood.

1. Plateletpheresis using the haemonetics model 30 cell separator.

Review 2. Resistance of cultured human fibroblasts and other cells to purine and pyrimidine analogues in relation to mutagenesis detection.

3. Variability of the time at which PHA-stimulated lymphocytes initiate DNA synthesis.

4. The spontaneous azaguanine-resistant mutants of diploid human fibroblasts.

5. Precipitation of radioactively labeled samples: a semi-automatic multiple-sample processor.

6. Genetic studies of HG- PRT deficiency and the Lesch-Nyhan syndrome with cultured human cells.

Review 7. Selective systems in somatic cell genetics.

8. Enzyme defect associated with a sex-linked human neurological disorder and excessive purine synthesis.

9. Phenotypic lag and mutation to 6-thioguanine resistance in diploid human lymphoblasts.

10. Enumeration of 6-thioguanine-resistant peripheral blood lymphocytes in man as a potential test for somatic cell mutations arising in vivo.

1. Influence of sex, smoking and age on human hprt mutation frequencies and spectra.

2. Bloom's syndrome. XVIII. Hypermutability at a tandem-repeat locus.

3. Mutation rates at the glycophorin A and HPRT loci in uranium miners exposed to radon progeny.

Review 4. A high-fidelity method for genomic sequencing of single somatic cells reveals a very high mutational burden.

Review 5. Detection of single base changes in nucleic acids.

6. Mutations in human lymphocytes commonly involve gene duplication and resemble those seen in cancer cels.

7. Molecular analyses of a Lesch-Nyhan syndrome mutation (hprtMontreal) by use of T-lymphocyte cultures.

8. Spontaneously arising red cells with a McLeod-like phenotype in normal donors.

9. Measurements of the frequency of human erythrocytes with gene expression loss phenotypes at the glycophorin A locus.

10. Homologies between T cell receptor junctional sequences unique to multiple sclerosis and T cells mediating experimental allergic encephalomyelitis.