Literature DB >> 6979559

A simple, rapid ELISA method for the detection of DNA antibodies.

R P Stokes, A Cordwell, R A Thompson.   

Abstract

Employing an enzyme-linked immunosorbent assay (ELISA) technique the serum antibodies against native (double stranded) and denatured (single stranded) deoxyribonucleic acid (DNA) have been measured in various disease groups and a group of blood donor sera. The ELISA method has been compared with a radioimmunoassay method using native (double stranded) DNA is substrate antigen and a latex-fixation technique using particles coated with soluble deoxyribonucleoprotein (SNP). It is concluded that ELISA offers an economic and reliable alternative to isotope techniques for the assessment of antibody content in systemic lupus erythematosus (SLE) and related disease states for the clinical laboratory.

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Year:  1982        PMID： 6979559      PMCID： PMC497719          DOI： 10.1136/jcp.35.5.566

Source DB:  PubMed          Journal:  J Clin Pathol        ISSN： 0021-9746            Impact factor:   3.411


  33 in total

1.  The relation of immunoglobulin class, pattern of anti-nuclear antibody, and complement-fixing antibodies to DNA in sera from patients with systemic lupus erythematosus.

Authors:  N F Rothfield; B D Stollar
Journal:  J Clin Invest       Date:  1967-11       Impact factor: 14.808

2.  Character of anti-DNA antibodies in systemic lupus erythematosus.

Authors:  S A Cohen; G R Hughes; G L Noel; C L Christian
Journal:  Clin Exp Immunol       Date:  1971-04       Impact factor: 4.330

3.  Immunologic factors and clinical activity in systemic lupus erythematosus.

Authors:  P H Schur; J Sandson
Journal:  N Engl J Med       Date:  1968-03-07       Impact factor: 91.245

4.  Antibodies to native DNA in systemic lupus erythematosus. A technique of rapid and quantitative determination.

Authors:  N M Kredich; J S Skyler; L J Foote
Journal:  Arch Intern Med       Date:  1973-05

5.  Deoxyribonucleic acid antibody: a method to detect its primary interaction with deoxyribonucleic acid.

Authors:  R T Wold; F E Young; E M Tan; R S Farr
Journal:  Science       Date:  1968-08-23       Impact factor: 47.728

6.  An immunologic precipitin system between soluble nucleoprotein and serum antibody in systemic lupus erythematosus.

Authors:  E M Tan
Journal:  J Clin Invest       Date:  1967-05       Impact factor: 14.808

7.  Deoxybonucleic acid (DNA) and antibodies to DNA in the serum of patients with systemic lupus erythematosus.

Authors:  E M Tan; P H Schur; R I Carr; H G Kunkel
Journal:  J Clin Invest       Date:  1966-11       Impact factor: 14.808

8.  Studies on DNA antibodies using DNA labelled with actinomycin-D (3H) or dimethyl (3H) sulphate.

Authors:  R I Carr; D Koffler; V Agnello; H G Kunkel
Journal:  Clin Exp Immunol       Date:  1969-05       Impact factor: 4.330

9.  Measurement of serum DNA-binding activity in systemic lupus erythematosus.

Authors:  T Pincus; P H Schur; J A Rose; J L Decker; N Talal
Journal:  N Engl J Med       Date:  1969-09-25       Impact factor: 91.245

10.  Antibodies to polynucleotides in human sera: antigenic specificity and relation to disease.

Authors:  D Koffler; R Carr; V Agnello; R Thoburn; H G Kunkel
Journal:  J Exp Med       Date:  1971-07-01       Impact factor: 14.307
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  9 in total

1.  A microfluidic competitive immuno-aggregation assay for high sensitivity cell secretome detection.

Authors:  Fan Liu; Pawan Kc; Liwei Ni; Ge Zhang; Jiang Zhe
Journal:  Organogenesis       Date:  2018-06-08       Impact factor: 2.500

2.  Reactive oxygen species modify human DNA, eliciting a more discriminating antigen for the diagnosis of systemic lupus erythematosus.

Authors:  S Blount; H Griffiths; P Emery; J Lunec
Journal:  Clin Exp Immunol       Date:  1990-09       Impact factor: 4.330

3.  Nonlinear behaviour of anti-dsDNA antibodies in dilution experiments using ultramicro ELISA.

Authors:  U Wollina
Journal:  Arch Dermatol Res       Date:  1985       Impact factor: 3.017

Review 4.  Antinuclear antibody testing - misunderstood or misbegotten?

Authors:  David S Pisetsky
Journal:  Nat Rev Rheumatol       Date:  2017-05-25       Impact factor: 20.543

5.  Antibodies to dsDNA in connective tissue diseases measured by ultramicro enzyme-linked immunosorbent assay (ELISA) adapted to the chamber analytical technique (CAT).

Authors:  U Wollina
Journal:  Arch Dermatol Res       Date:  1984       Impact factor: 3.017

6.  Enzyme-linked immunosorbent assays for antibodies to poly(A), poly dAT and histones: possibly useful tools for the evaluation of prognosis and disease activity in systemic lupus erythematosus.

Authors:  T Helve; M Gripenberg; P Kurki; A M Teppo; O Wegelius
Journal:  Rheumatol Int       Date:  1984       Impact factor: 2.631

7.  Measurement of serum DNA binding in chronic active hepatitis and systemic lupus erythematosus using the Farr assay.

Authors:  K M Pollard; R Steele; S Hogg; J Webb
Journal:  Rheumatol Int       Date:  1986       Impact factor: 2.631

8.  Distribution of an anti-DNA idiotype among autoantibodies in patients with systemic lupus erythematosus (SLE) or rheumatoid arthritis (RA).

Authors:  G D Harkiss; F Hendrie; G Nuki
Journal:  J Clin Immunol       Date:  1986-07       Impact factor: 8.317

9.  The Use of Poly-L-Lysine as a Capture Agent to Enhance the Detection of Antinuclear Antibodies by ELISA.

Authors:  Nancy A Stearns; Shuxia Zhou; Michelle Petri; Steven R Binder; David S Pisetsky
Journal:  PLoS One       Date:  2016-09-09       Impact factor: 3.240
  9 in total

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