Literature DB >> 6978487

Purification and characterization of a monoclonal T-cell suppressor factor specific for poly(LGlu60LAla30LTyr10).

K Krupen, B A Araneo, L Brink, J A Kapp, S Stein, K J Wieder, D R Webb.   

Abstract

A monoclonal T-cell-derived suppressor factor specific for the terpolymer poly(LGlu60LAla30LTyr10) produced by the T-cell hybridoma 258 C4.4, was purified to homogeneity. This was accomplished by fractionation of the culture medium by using a combination of affinity chromatography and reverse-phase and ion-exchange high-performance liquid chromatography. The purified factor is composed of a single Mr 24,000 polypeptide chain, and the homogeneous protein maintains the ability to suppress antibody and T-cell proliferative responses to poly(LGlu60LAla30LTyr10) specifically. The specific activity of pure suppressor factor is calculated to be 8 X 10(7) units/micrograms.

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Year:  1982        PMID： 6978487      PMCID： PMC345940          DOI： 10.1073/pnas.79.4.1254

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  26 in total

1.  Automatic Monitoring of primary amines in preparative column effluents with fluorescamine.

Authors:  P Böhlen; S Stein; J Stone; S Udenfriend
Journal:  Anal Biochem       Date:  1975-08       Impact factor: 3.365

2.  Amino acid analysis with fluorescamine at the picomole level.

Authors:  S Stein; P Böhlen; J Stone; W Dairman; S Udenfriend
Journal:  Arch Biochem Biophys       Date:  1973-03       Impact factor: 4.013

3.  Mapping the H-2-linked genes governing, respectively, the immune responses to a glutamic acid-alanine-tyrosine copolymer and to limiting doses of ovalbumin.

Authors:  E K Dunham; M E Dorf; D C Shreffler; B Benacerraf
Journal:  J Immunol       Date:  1973-12       Impact factor: 5.422

4.  Molecular weight determination of protein-dodecyl sulfate complexes by gel electrophoresis in a discontinuous buffer system.

Authors:  D M Neville
Journal:  J Biol Chem       Date:  1971-10-25       Impact factor: 5.157

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  Immunosuppressive factor(s) extracted from lymphoid cells of nonresponder mice primed with L-glutamic acid60-L-alanine30-L-tyrosine10 (GAT).

Authors:  J A Kapp; C W Pierce; F De la Croix; B Benacerraf
Journal:  J Immunol       Date:  1976-02       Impact factor: 5.422

7.  A cellular basis for genetically controlled immunologic unresponsiveness in mice: tolerance induction in T-cells.

Authors:  R K Gershon; P H Maurer; C F Merryman
Journal:  Proc Natl Acad Sci U S A       Date:  1973-01       Impact factor: 11.205

8.  Genetic control of immune responses in vitro. I. Development of primary and secondary plaque-forming cell responses to the random terpolymer 1-glutamic acid 60-1-alanine30-1-tyrosine10 (GAT) by mouse spleen cells in vitro.

Authors:  J A Kapp; C W Pierce; B Benacerraf
Journal:  J Exp Med       Date:  1973-11-01       Impact factor: 14.307

9.  Immunosuppressive factor(s) extracted from lymphoid cells of nonresponder mice primed with L-glutamic acid60-L-alanine30-L-tyrosine10 (GAT) II. Cellular source and effect on responder and nonresponder mice.

Authors:  J A Kapp; C W Pierce; B Benacerraf
Journal:  J Exp Med       Date:  1977-04-01       Impact factor: 14.307

10.  Genetic control of immune responses in vitro. V. Stimulation of suppressor T cells in nonresponder mice by the terpolymer L-glutamic acid 60-L-alanine 30-L-tyrosine 10 (GAT).

Authors:  J A Kapp; C W Pierce; S Schlossman; B Benacerraf
Journal:  J Exp Med       Date:  1974-09-01       Impact factor: 14.307
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  17 in total

Review 1.  Special regulatory T-cell review: A rose by any other name: from suppressor T cells to Tregs, approbation to unbridled enthusiasm.

Authors:  Ronald N Germain
Journal:  Immunology       Date:  2008-01       Impact factor: 7.397

2.  Dengue virus-induced helper cytokine has two polypeptide chains which bear different determinants.

Authors:  P Chaturvedi; R Mukherjee; U C Chaturvedi; A Mathur
Journal:  Int J Exp Pathol       Date:  1991-12       Impact factor: 1.925

3.  Molecular genetic characterization of the mRNA coding for an inducible suppressor factor specific for L-glutamic acid60-L-alanine30-L-tyrosine10.

Authors:  C L Funckes-Shippy; C M Sorensen; C W Pierce; A D Levine
Journal:  Proc Natl Acad Sci U S A       Date:  1987-09       Impact factor: 11.205

4.  Binding of the two polypeptide chains of dengue virus-induced suppressor cytokine to its receptor isolated from macrophages.

Authors:  R Mukherjee; P Chaturvedi; U C Chaturvedi
Journal:  Int J Exp Pathol       Date:  1993-06       Impact factor: 1.925

5.  Structural analysis of antigen-specific Ia-bearing regulatory T-cell factors: gel electrophoretic analysis of the antigen-specific augmenting T -cell factor.

Authors:  S Miyatani; K Hiramatsu; P B Nakajima; F L Owen; T Tada
Journal:  Proc Natl Acad Sci U S A       Date:  1983-10       Impact factor: 11.205

6.  Cell-free translation of a biologically active, antigen-specific suppressor T cell factor.

Authors:  K J Wieder; B A Araneo; J A Kapp; D R Webb
Journal:  Proc Natl Acad Sci U S A       Date:  1982-06       Impact factor: 11.205

7.  Partial purification and characterization of an antigen-specific helper factor synthesized by a T-cell continuous line.

Authors:  R Lifshitz; R N Apte; E Mozes
Journal:  Proc Natl Acad Sci U S A       Date:  1983-09       Impact factor: 11.205

8.  Purification and characterization of an L-glutamic acid60-L-alanine30-L-tyrosine10 (GAT)-specific suppressor factor from genetic responder mice.

Authors:  C M Sorensen; C W Pierce; D R Webb
Journal:  J Exp Med       Date:  1983-10-01       Impact factor: 14.307

9.  RNA transcripts for I-J polypeptides are apparently not encoded between the I-A and I-E subregions of the murine major histocompatibility complex.

Authors:  M Kronenberg; M Steinmetz; J Kobori; E Kraig; J A Kapp; C W Pierce; C M Sorensen; G Suzuki; T Tada; L Hood
Journal:  Proc Natl Acad Sci U S A       Date:  1983-09       Impact factor: 11.205

10.  Human T cell hybridomas secreting immune interferon.

Authors:  J Le; J Vilcek; C Saxinger; W Prensky
Journal:  Proc Natl Acad Sci U S A       Date:  1982-12       Impact factor: 11.205
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