| Literature DB >> 6972416 |
Abstract
A simple and rapid method for the production of TCGF free of lectin (LF-TCGF) is described. Murine splenocytes are exposed to 10--20 micrograms/ml Con A for 2 h before washing the cells and incubating them in medium for an additional 20 h. This procedure leads to the same titers of TCGF activity obtained when Con A is presented throughout the incubation time. The resulting TCGF preparation is free of Con A as evidenced by its inability to activate fresh resting lymphoid cells and by measurements using [3H]Con A in tracer amounts. The LF-TCGF is capable of growing activated but not resting lymphocytes and can lead to greater than 50-fold increase in the generation of cytotoxic cells in in vitro sensitizations to alloantigens.Entities:
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Year: 1981 PMID： 6972416 DOI： 10.1016/0022-1759(81)90151-4
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303