Literature DB >> 6950381

Nucleotide sequence of cloned cDNAs encoding human preproparathyroid hormone.

G N Hendy, H M Kronenberg, J T Potts, A Rich.   

Abstract

We have cloned cDNA copies of human preproparathyroid hormone in Escherichia coli after insertion of double-stranded DNA into the Pst I site of plasmid pBR322 using the poly(dG) . poly(dC) homopolymer extension technique. Recombinant plasmids coding for preproparathyroid hormone were identified by filter hybridization assay using as a probe 32P-labeled bovine preproparathyroid cDNA. Nucleotide sequence analysis of five recombinant plasmids permitted the assignment of 74 nucleotides of the 5' noncoding region, the entire coding region of 345 nucleotides, and the entire 3' noncoding region of 348 nucleotides of the mRNA. The coding sequence predicts the previously unknown "pre" amino acid sequence and clarifies the hormone's amino acid sequence, which has been disrupted. The 5' noncoding region contains an AUG codon followed by a UGA stop codon before the authentic initiator codon. The 3' noncoding region is 120 nucleotides longer than in bovine preproparathyroid mRNA and contains two A-A-U-A-A-A sequences, potential signals for polyadenylation.

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Year:  1981        PMID： 6950381      PMCID： PMC349267          DOI： 10.1073/pnas.78.12.7365

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  34 in total

1.  Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.

Authors:  M Grunstein; D S Hogness
Journal:  Proc Natl Acad Sci U S A       Date:  1975-10       Impact factor: 11.205

2.  Polynucleotide kinase exchange reaction: quantitave assay for restriction endonuclease-generated 5'-phosphoroyl termini in DNA.

Authors:  K L Berkner; W R Folk
Journal:  J Biol Chem       Date:  1977-05-25       Impact factor: 5.157

3.  The NH2-terminal amino acid sequence of human proparathyroid hormone by radioisotope microanalysis.

Authors:  W Y Huang; L L Chu; J W Hamilton; D H McGregor; D V Cohn
Journal:  Arch Biochem Biophys       Date:  1975-01       Impact factor: 4.013

4.  Sequence at the 3' end of globin mRNA shows homology with immunoglobulin light chain mRNA.

Authors:  N J Proudfoot; G G Brownlee
Journal:  Nature       Date:  1974-11-29       Impact factor: 49.962

5.  The amino-acid sequence of the amino-terminal 37 residues of human parathyroid hormone.

Authors:  H D Niall; R T Sauer; J W Jacobs; H T Keutmann; G V Segre; J L O'Riordan; G D Aurbach; J T Potts
Journal:  Proc Natl Acad Sci U S A       Date:  1974-02       Impact factor: 11.205

6.  Structural analysis of human proparathyroid hormone by a new microsequencing approach.

Authors:  J W Jacobs; B Kemper; H D Niall; J F Habener; J T Potts
Journal:  Nature       Date:  1974-05-10       Impact factor: 49.962

7.  Pre - proparathyroid hormone identified by cell - free translation of messenger RNA from hyperplastic human parathyroid tissue.

Authors:  J F Habener; B Kemper; J T Potts; A Rich
Journal:  J Clin Invest       Date:  1975-11       Impact factor: 14.808

8.  A reinvestigation of the amino-terminal sequence of human parathyroid hormone.

Authors:  H T Keutmann; H D Niall; J L O'Riordan; J T Potts
Journal:  Biochemistry       Date:  1975-05-06       Impact factor: 3.162

9.  Human parathyroid hormone: amino-acid sequence of the amino-terminal residues 1-34.

Authors:  H B Brewer; T Fairwell; R Ronan; G W Sizemore; C D Arnaud
Journal:  Proc Natl Acad Sci U S A       Date:  1972-12       Impact factor: 11.205

10.  Terminal labeling and addition of homopolymer tracts to duplex DNA fragments by terminal deoxynucleotidyl transferase.

Authors:  R Roychoudhury; E Jay; R Wu
Journal:  Nucleic Acids Res       Date:  1976-01       Impact factor: 16.971
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  37 in total

1.  Tissue-specific regulatory regions of the PTH gene localized by novel chromosome 11 rearrangement breakpoints in a parathyroid adenoma.

Authors:  Sanjay M Mallya; H Irene Wu; Elizabeth A Saria; Kristin R Corrado; Andrew Arnold
Journal:  J Bone Miner Res       Date:  2010-07-16       Impact factor: 6.741

2.  Linkage analysis of neurofibromatosis.

Authors:  S Kittur; M L Lubs; M Bauer; A Chakravarti; H Kazazian
Journal:  J Med Genet       Date:  1987-09       Impact factor: 6.318

3.  In vitro expression of two proteins from overlapping reading frames in a eukaryotic DNA sequence.

Authors:  J M Jankowski; S A Krawetz; E Walczyk; G H Dixon
Journal:  J Mol Evol       Date:  1986       Impact factor: 2.395

4.  Dynein light chain binding to a 3'-untranslated sequence mediates parathyroid hormone mRNA association with microtubules.

Authors:  E Epstein; A Sela-Brown; I Ringel; R Kilav; S M King; S E Benashski; J K Yisraeli; J Silver; T Naveh-Many
Journal:  J Clin Invest       Date:  2000-02       Impact factor: 14.808

5.  Hemoglobin F production in heterocellular hereditary persistence of fetal hemoglobin and its linkage to the beta globin gene complex.

Authors:  J A Donald; A Lammi; R J Trent
Journal:  Hum Genet       Date:  1988-09       Impact factor: 4.132

6.  Nucleotide sequence of a full-length cDNA clone encoding preproparathyroid hormone from pig and rat.

Authors:  H J Schmelzer; G Gross; G Widera; H Mayer
Journal:  Nucleic Acids Res       Date:  1987-08-25       Impact factor: 16.971

7.  Parathyroid hormone expression in a patient with metastatic nasopharyngeal rhabdomyosarcoma and hypercalcemia.

Authors:  Katy Wong; Shawn Tsuda; Ryan Mukai; Kenneth Sumida; Richard Arakaki
Journal:  Endocrine       Date:  2005-06       Impact factor: 3.633

8.  Sequences of the 5' portion of the human c-sis gene: characterization of the transcriptional promoter and regulation of expression of the protein product by 5' untranslated mRNA sequences.

Authors:  L Ratner; B Thielan; T Collins
Journal:  Nucleic Acids Res       Date:  1987-08-11       Impact factor: 16.971

9.  Secretion of human parathyroid hormone from rat pituitary cells infected with a recombinant retrovirus encoding preproparathyroid hormone.

Authors:  J G Hellerman; R C Cone; J T Potts; A Rich; R C Mulligan; H M Kronenberg
Journal:  Proc Natl Acad Sci U S A       Date:  1984-09       Impact factor: 11.205

10.  Cloning and sequence analysis of the human parathyroid hormone gene region.

Authors:  A Reis; W Hecht; R Gröger; I Böhm; D N Cooper; W Lindenmaier; H Mayer; J Schmidtke
Journal:  Hum Genet       Date:  1990-01       Impact factor: 4.132
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