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Literature DB >> 6941285

Mouse beta-globin and adenovirus-2 major late transcripts are initiated at the cap site in vitro.

Abstract

Initiation of transcription on major late DNA of adenovirus serotype 2 and the beta-globin gene of the mouse was studied by using ribonucleoside [beta-32P]triphosphates as labeled precursors in vitro. The 5' termini of discrete runoff transcripts of both templates were preferentially labeled. The beta phosphate of the cap structure was labeled by [beta-32P]ATP but not [beta-32P]GTP, indicating that the beta phosphate of the cap is contributed by the initiating nucleoside triphosphate (ATP in both cases). Therefore, capping occurs on the initial nucleotide of these transcripts.

Entities: Chemical Species

Mesh：

Substances：
RNA Caps
Globins

Year: 1981 PMID： 6941285 PMCID： PMC319329 DOI： 10.1073/pnas.78.4.2283

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

39 in total

1. An intercistronic region and ribosome-binding site in bacterial messenger RNA.

Authors: T Platt; C Yanofsky
Journal: Proc Natl Acad Sci U S A Date: 1975-06 Impact factor: 11.205

2. The 5' terminal structure of the methylated mRNA synthesized in vitro by vesicular stomatitis virus.

Authors: G Abraham; D P Rhodes; A K Banerjee
Journal: Cell Date: 1975-05 Impact factor: 41.582

3. Modification of the 5' end of mRNA. Association of RNA triphosphatase with the RNA guanylyltransferase-RNA (guanine-7-)methyltransferase complex from vaccinia virus.

Authors: S Venkatesan; A Gershowitz; B Moss
Journal: J Biol Chem Date: 1980-02-10 Impact factor: 5.157

4. Direct determination of DNA nucleotide sequences: structure of a fragment of bacteriophage phiX172 DNA.

Authors: F Galibert; J Sedat; E Ziff
Journal: J Mol Biol Date: 1974-08-15 Impact factor: 5.469

5. Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S beta-globin mRNA precursor and mature 10 s beta-globin mRNA have identical map coordinates.

Authors: R F Weaver; C Weissmann
Journal: Nucleic Acids Res Date: 1979-11-10 Impact factor: 16.971

6. An improved method for thin-layer chromatography of nucleotide mixtures containing 32P-labelled orthophosphate.

Authors: M Cashel; R A Lazzarini; B Kalbacher
Journal: J Chromatogr Date: 1969-03-11

7. The ovalbumin gene-sequence of putative control regions.

Authors: C Benoist; K O'Hare; R Breathnach; P Chambon
Journal: Nucleic Acids Res Date: 1980-01-11 Impact factor: 16.971

8. The addition of 5' cap structures occurs early in hnRNA synthesis and prematurely terminated molecules are capped.

Authors: M Salditt-Georgieff; M Harpold; S Chen-Kiang; J E Darnell
Journal: Cell Date: 1980-01 Impact factor: 41.582

9. Donor and acceptor specificities of HeLa cell mRNA guanylyltransferase.

Authors: S Venkatesan; B Moss
Journal: J Biol Chem Date: 1980-04-10 Impact factor: 5.157

10. Modification of the 5'-terminus of mRNA by soluble guanylyl and methyl transferases from vaccinia virus.

Authors: M J Ensinger; S A Martin; E Paoletti; B Moss
Journal: Proc Natl Acad Sci U S A Date: 1975-07 Impact factor: 11.205

19 in total

1. Synthesis of U1 RNA in isolated nuclei from sea urchin embryos: U1 RNA is initiated at the first nucleotide of the RNA.

Authors: G F Morris; W F Marzluff
Journal: Mol Cell Biol Date: 1985-05 Impact factor: 4.272

Mouse beta-globin and adenovirus-2 major late transcripts are initiated at the cap site in vitro.

1. An intercistronic region and ribosome-binding site in bacterial messenger RNA.

2. The 5' terminal structure of the methylated mRNA synthesized in vitro by vesicular stomatitis virus.

3. Modification of the 5' end of mRNA. Association of RNA triphosphatase with the RNA guanylyltransferase-RNA (guanine-7-)methyltransferase complex from vaccinia virus.

4. Direct determination of DNA nucleotide sequences: structure of a fragment of bacteriophage phiX172 DNA.

5. Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S beta-globin mRNA precursor and mature 10 s beta-globin mRNA have identical map coordinates.

6. An improved method for thin-layer chromatography of nucleotide mixtures containing 32P-labelled orthophosphate.

7. The ovalbumin gene-sequence of putative control regions.

8. The addition of 5' cap structures occurs early in hnRNA synthesis and prematurely terminated molecules are capped.

9. Donor and acceptor specificities of HeLa cell mRNA guanylyltransferase.

10. Modification of the 5'-terminus of mRNA by soluble guanylyl and methyl transferases from vaccinia virus.

1. Synthesis of U1 RNA in isolated nuclei from sea urchin embryos: U1 RNA is initiated at the first nucleotide of the RNA.

2. Transcription termination at the chicken beta H-globin gene.

3. DNA sequences required for specific and efficient initiation of transcription at the polyoma virus early promoter.

4. Processing and nucleo-cytoplasmic transport of histone gene transcripts.

5. Nuclear beta-globin RNA transcripts initiated in vitro with gamma-thio nucleoside triphosphate.

6. Comparison of the polyoma virus early and late promoters by transcription in vitro.

7. Transcription of the beta-like globin genes and pseudogenes of the goat in a cell-free system.

8. Sequences at the capped 5'-ends of polyoma virus late region mRNAs: an example of extreme terminal heterogeneity.

9. Changes in the topography of early region transcription during polyoma virus lytic infection.

10. An immunoglobulin promoter region is unaltered by DNA rearrangement and somatic mutation during B-cell development.