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Literature DB >> 6897113

The location of the first AUG codons in cowpea mosaic virus RNAs.

G P Lomonossoff, M Shanks, H D Matthes, M Singh, M J Gait.

Abstract

We have made use of the known sequence of the 5' ends of both CPMV RNAs to synthesise an oligodeoxynucleotide which can prime second-strand DNA synthesis on full-length cDNA copies of both RNAs. By priming synthesis in the presence of dideoxynucleoside triphosphates, we have determined the positions of the first AUG codons in each RNA. These occur at positions 115 and 207 on M and B RNA respectively. By using a cloned double-stranded DNA fragment derived from near the 5' end of M RNA as a primer additional sequence from the 5' terminal region of M RNA has been obtained.

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Year: 1982 PMID： 6897113 PMCID： PMC320829 DOI： 10.1093/nar/10.16.4861

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

37 in total

1. Nucleotide sequences adjacent to the proteins covalently linked to the cowpea mosaic virus genome. Sequence determination after labelling in vitro using RNA ligase.

Authors: J Stanley; A Van Kammen
Journal: Eur J Biochem Date: 1979-11-01

2. Protein bound to the genome RNAs of cowpea mosaic virus.

Authors: S D Daubert; G Bruening; R C Najarian
Journal: Eur J Biochem Date: 1978-12-01

3. The use of nuclease P1 in sequence analysis of end group labeled RNA.

Authors: M Silberklang; A M Gillum; U L RajBhandary
Journal: Nucleic Acids Res Date: 1977-12 Impact factor: 16.971

4. Sequence homology adjacent to the 3' terminal poly(A) of cowpea mosaic virus RNAs.

Authors: J W Davies; J Stanley; A Van Kammen
Journal: Nucleic Acids Res Date: 1979-09-25 Impact factor: 16.971

5. Polypeptide cleavages in the formation of poliovirus proteins.

Authors: M F Jacobson; D Baltimore
Journal: Proc Natl Acad Sci U S A Date: 1968-09 Impact factor: 11.205

6. Sequence of 1060 3'-terminal nucleotides of poliovirus RNA as determined by a modification of the dideoxynucleotide method.

Authors: N Kitamura; E Wimmer
Journal: Proc Natl Acad Sci U S A Date: 1980-06 Impact factor: 11.205

7. Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes.

Authors: M Kozak
Journal: Nucleic Acids Res Date: 1981-10-24 Impact factor: 16.971

8. Polyadenylic acid at the 3'-terminus of poliovirus RNA.

Authors: Y Yogo; E Wimmer
Journal: Proc Natl Acad Sci U S A Date: 1972-07 Impact factor: 11.205

9. Binding of ribosomes to the 5' leader sequence (N = 258) of RNA 3 from alfalfa mosaic virus.

Authors: M Pinck; C Fritsch; M Ravelonandro; C Thivent; L Pinck
Journal: Nucleic Acids Res Date: 1981-03-11 Impact factor: 16.971

10. Complete nucleotide sequences of the coat protein messenger RNAs of brome mosaic virus and cowpea chlorotic mottle virus.

Authors: R Dasgupta; P Kaesberg
Journal: Nucleic Acids Res Date: 1982-01-22 Impact factor: 16.971

5 in total

1. The complete nucleotide sequence of PEBV RNA2 reveals the presence of a novel open reading frame and provides insights into the structure of tobraviral subgenomic promoters.

Authors: M G Goulden; G P Lomonossoff; J W Davies; K R Wood
Journal: Nucleic Acids Res Date: 1990-08-11 Impact factor: 16.971