Literature DB >> 6885982

Rapid quantitative microenzyme-linked immunosorbent assay for tetanus antibodies.

A K Sedgwick, M Ballow, K Sparks, R C Tilton.   

Abstract

A rapid quantitative microenzyme-linked immunosorbent assay for the detection of immunoglobulin G antibodies to tetanus toxoid is described. The assay is performed in 2.5 h and detects antibody levels of between 0.1 and 6 U/ml. Quantitation of the antibody concentration is unique in that multiple serum dilutions are tested on a single patient, the results are plotted as an antibody dilution curve, and the area under the curve is integrated for a final concentration. Tests for immune status on sera from 77 individuals demonstrated tetanus antibody concentrations of greater than or equal to 0.1 U/ml in 92% of a pediatric group and 62% of an adult group. The microenzyme-linked immunosorbent assay procedure is reproducible and is useful for the determination of immune status and immunization response.

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Year:  1983        PMID： 6885982      PMCID： PMC270751          DOI： 10.1128/jcm.18.1.104-109.1983

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  12 in total

1.  Immunoassay using antigen-enzyme conjugates.

Authors:  B K. Van Weemen; A H.W.M. Schuurs
Journal:  FEBS Lett       Date:  1971-06-24       Impact factor: 4.124

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Authors:  G Stiffler-Rosenberg; H Fey
Journal:  Schweiz Med Wochenschr       Date:  1975-06-21

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Authors:  S L Bullock; K W Walls
Journal:  J Infect Dis       Date:  1977-10       Impact factor: 5.226

4.  A rapid and simple radioimmunological procedure for measuring low concentrations of tetanus antibodies.

Authors:  E Habermann; H Wiegand
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  1973       Impact factor: 3.000

5.  Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G.

Authors:  E Engvall; P Perlmann
Journal:  Immunochemistry       Date:  1971-09

6.  A micro-enzyme-linked immunosorbent assay (ELISA) and radioimmunosorbent technique (RIST) for the detection of immunity to clinical tetanus.

Authors:  G T Layton
Journal:  Med Lab Sci       Date:  1980-10

7.  The communication of ELISA data from laboratory to clinician.

Authors:  D de Savigny; A Voller
Journal:  J Immunoassay       Date:  1980

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Authors:  E Habermann; E Horváth; W Schaeg
Journal:  Med Microbiol Immunol       Date:  1977-12-27       Impact factor: 3.402

9.  A rapid automated latex screen for tetanus toxoid antibodies.

Authors:  J R Booth; P A Nuttall
Journal:  Vox Sang       Date:  1978       Impact factor: 2.144

10.  Immunization against neonatal tetanus in New Guinea. 3. The toxin-neutralization test and the response of guinea-pigs to the toxoids as used in the immunization schedules in New Guinea.

Authors:  M F Barile; M C Hardegree; M Pittman
Journal:  Bull World Health Organ       Date:  1970       Impact factor: 9.408
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  13 in total

1.  Antigenically diverse reference strains and autologous strains of Actinobacillus actinomycetemcomitans are equally efficient antigens in enzyme-linked immunosorbent assay analysis.

Authors:  Tiina Vilkuna-Rautiainen; Pirkko J Pussinen; Kimmo Mattila; Marja Vesanen; Heidi Ahman; Başak Doğan; Sirkka Asikainen
Journal:  J Clin Microbiol       Date:  2002-12       Impact factor: 5.948

2.  Role of Rapid Tetanus Antibody Test in Accident and Emergency Department.

Authors:  A Chithra; K M Cariappa; Abhay Taranath Kamath; Adarsh Kudva
Journal:  J Maxillofac Oral Surg       Date:  2014-10-14

3.  Multiserotype enzyme-linked immunosorbent assay as a diagnostic aid for periodontitis in large-scale studies.

Authors:  P J Pussinen; T Vilkuna-Rautiainen; G Alfthan; K Mattila; S Asikainen
Journal:  J Clin Microbiol       Date:  2002-02       Impact factor: 5.948

4.  A common anti-cardiolipin antibody idiotype in autoimmune disease: identification using a mouse monoclonal antibody directed against a naturally-occurring anti-phospholipid antibody.

Authors:  M Sutjita; A Hohmann; R Comacchio; M L Boey; J Bradley
Journal:  Clin Exp Immunol       Date:  1989-02       Impact factor: 4.330

5.  Tubal infertility in the Gambia: chlamydial and gonococcal serology in women with tubal occlusion compared with pregnant controls.

Authors:  D C Mabey; G Ogbaselassie; J N Robertson; J E Heckels; M E Ward
Journal:  Bull World Health Organ       Date:  1985       Impact factor: 9.408

6.  Measurement of diphtheria and tetanus antitoxin in blood samples collected on filter paper disks.

Authors:  S Nikoletti
Journal:  Epidemiol Infect       Date:  1994-02       Impact factor: 2.451

7.  Study of anti tetanus IgG and IgM levels in maternal and cord sera by enzyme linked immunosorbent assay (ELISA).

Authors:  S Sundar; N Wagle; B L Varudkar; A Vaidya; S M Merchant
Journal:  Indian J Pediatr       Date:  1990 Nov-Dec       Impact factor: 1.967

8.  Allergen-specific IgG1 provides parsimonious heritability estimates for atopy-associated immune responses to allergens.

Authors:  Carol L Liebeler; Saonli Basu; Duaine R Jackola
Journal:  Hum Immunol       Date:  2006-12-28       Impact factor: 2.850

9.  Detection of tetanus toxoid antibodies in human sera in New Zealand by ELISA.

Authors:  R C Lau
Journal:  Epidemiol Infect       Date:  1987-04       Impact factor: 2.451

10.  Detection of tetanus antitoxin using Eu(3+)-labeled anti-human immunoglobulin G monoclonal antibodies in a time-resolved fluorescence immunoassay.

Authors:  J P Schröder; W D Kuhlmann
Journal:  J Clin Microbiol       Date:  1991-07       Impact factor: 5.948
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