Literature DB >> 6884525

Intracellular pH and free calcium changes in single cells using quene 1 and quin 2 probes and fluorescence microscopy.

J Rogers, T R Hesketh, G A Smith, M A Beaven, J C Metcalfe, P Johnson, P B Garland.   

Abstract

Photometric fluorescence microscopy has been used to measure intracellular pH (pHi) and free calcium concentrations [( Ca]i) in individual mouse thymocytes and 2H3 rat basophil leukaemic cells containing indicators for pH (quene 1) or calcium (quin 2). The pHi and [Ca]i measurements in individual 2H3 cells and mouse thymocytes and their responses to various stimuli were consistent with the corresponding data obtained from suspensions of these cells measured in a spectrofluorimeter. Photometric fluorescence microscopy of these indicators in individual cells provides a sensitive and fast method of following pHi and [Ca]i responses in individual cells.

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Year:  1983        PMID： 6884525     DOI： 10.1016/0014-5793(83)80722-4

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  12 in total

1.  Fluorescence lifetime imaging.

Authors:  J R Lakowicz; H Szmacinski; K Nowaczyk; K W Berndt; M Johnson
Journal:  Anal Biochem       Date:  1992-05-01       Impact factor: 3.365

2.  Imaging asynchronous changes in intracellular Ca2+ in individual stimulated tumor mast cells.

Authors:  P J Millard; D Gross; W W Webb; C Fewtrell
Journal:  Proc Natl Acad Sci U S A       Date:  1988-03       Impact factor: 11.205

Review 3.  Mechanism of signal transduction in mast cells and basophils: studies with RBL-2H3 cells.

Authors:  M A Beaven; K Maeyama; E Wolde-Mussie; T N Lo; H Ali; J R Cunha-Melo
Journal:  Agents Actions       Date:  1987-04

4.  Design and properties of a fluorescent indicator of intracellular free Na+ concentration.

Authors:  G A Smith; T R Hesketh; J C Metcalfe
Journal:  Biochem J       Date:  1988-02-15       Impact factor: 3.857

5.  Intracellular free calcium levels are reduced in mitotic Pt K2 epithelial cells.

Authors:  C H Keith; F R Maxfield; M L Shelanski
Journal:  Proc Natl Acad Sci U S A       Date:  1985-02       Impact factor: 11.205

6.  Requirements for entry of poliovirus RNA into cells at low pH.

Authors:  I H Madshus; S Olsnes; K Sandvig
Journal:  EMBO J       Date:  1984-09       Impact factor: 11.598

7.  Effects of cytoplasmic acidification on clathrin lattice morphology.

Authors:  J Heuser
Journal:  J Cell Biol       Date:  1989-02       Impact factor: 10.539

8.  Acidification of the cytosol inhibits endocytosis from coated pits.

Authors:  K Sandvig; S Olsnes; O W Petersen; B van Deurs
Journal:  J Cell Biol       Date:  1987-08       Impact factor: 10.539

9.  Changes in lysosome shape and distribution correlated with changes in cytoplasmic pH.

Authors:  J Heuser
Journal:  J Cell Biol       Date:  1989-03       Impact factor: 10.539

10.  Niclosamide is a proton carrier and targets acidic endosomes with broad antiviral effects.

Authors:  Andreas Jurgeit; Robert McDowell; Stefan Moese; Eric Meldrum; Reto Schwendener; Urs F Greber
Journal:  PLoS Pathog       Date:  2012-10-25       Impact factor: 6.823
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