Literature DB >> 687377

Heparin-sepharose 4B at low temperatures retains ribosomes.

J Hradec, O Kríz.   

Abstract

Ribosomes were separated by zonal ultracentrifugation from the fraction of reticulocyte postmitochondrial supernatants retained on heparin bound to Sepharose 4B at 4 degrees C. No ribosomes were retained at 22 degrees C. Ribosomes were also adsorbed on Sepharose 4B and 6B at low temperatures and could be eluted after increasing the column temperature.

Entities:  

Mesh：

Substances：

Year:  1978        PMID： 687377      PMCID： PMC1185782          DOI： 10.1042/bj1730349

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  8 in total

1.  The inhibition of elongation factor 1 activity by heparin.

Authors:  L I Slobin
Journal:  Biochem Biophys Res Commun       Date:  1976-12-06       Impact factor: 3.575

2.  Decreased activity of peptide-elongation factors after treatment with cholesterol esterase.

Authors:  J Hradec; Z Tuhácková; Z Dusek
Journal:  Biochem J       Date:  1978-04-15       Impact factor: 3.857

3.  Initiation of protein synthesis in eukaryotes. Binding to Sepharose-heparin and partial purification of initiation factors from Krebs II ascites cells.

Authors:  C Van der Mast; A Thomas; H Goumans; H Amesz; H O Voorma
Journal:  Eur J Biochem       Date:  1977-05-16

4.  Initiation of mammalian protein synthesis: the importance of ribosome and initiation factor quality for the efficiency of in vitro systems.

Authors:  M H Schreier; T Staehelin
Journal:  J Mol Biol       Date:  1973-02-19       Impact factor: 5.469

5.  Dissociation of mammalian polyribosomes into subunits by puromycin.

Authors:  G Blobel; D Sabatini
Journal:  Proc Natl Acad Sci U S A       Date:  1971-02       Impact factor: 11.205

6.  All factors required for protein synthesis are retained on heparin bound to Sepharose.

Authors:  J Hradec; Z Dusek
Journal:  Biochem J       Date:  1978-04-15       Impact factor: 3.857

7.  Influence of cholesteryl 14-methylhexadecanoate on some ribosomal functions required for peptide elongation.

Authors:  J Hradec; Z Dusek; O Mach
Journal:  Biochem J       Date:  1974-02       Impact factor: 3.857

8.  Isolation of rabbit reticulocyte initiation factors by means of heparin bound to sepharose.

Authors:  A A Waldman; G Marx; J Goldstein
Journal:  Proc Natl Acad Sci U S A       Date:  1975-06       Impact factor: 11.205
  8 in total
  1 in total

1.  Particulate aminoacyl-tRNA synthetases are retained on heparin bound to Sepharose.

Authors:  J Hradec; Z Dusek
Journal:  Mol Biol Rep       Date:  1980-12-31       Impact factor: 2.316
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.