Literature DB >> 6869835

Gel-exclusion chromatography on S1000 Sephacryl: application to phospholipid vesicles.

J A Reynolds, Y Nozaki, C Tanford.   

Abstract

Sephacryl S1000 has an exclusion diameter of approximately 3000 A and is thus an appropriate gel exclusion medium for size analysis and fractionation of phospholipid vesicles. Calibration is conveniently carried out using polystyrene beads of known diameter eluted with a detergent-containing buffer. Recovery of phospholipid vesicles of different sizes from S1000 presaturated with lipid is greater than 95%, and diameters obtained from a calibration curve using the polystyrene beads are in good agreement with those obtained by negative contrast electron microscopy.

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Year:  1983        PMID： 6869835     DOI： 10.1016/0003-2697(83)90618-8

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  12 in total

1.  Cellular uptake of a fluid-phase marker by human neutrophils from solutions and liposomes.

Authors:  J F Scieszka; M J Cho
Journal:  Pharm Res       Date:  1988-06       Impact factor: 4.200

2.  In vitro and in vivo studies evaluating a liposome system for drug solubilization.

Authors:  D M Lidgate; P L Felgner; J S Fleitman; J Whatley; R C Fu
Journal:  Pharm Res       Date:  1988-12       Impact factor: 4.200

Review 3.  A review of phosphatidate phosphatase assays.

Authors:  Prabuddha Dey; Gil-Soo Han; George M Carman
Journal:  J Lipid Res       Date:  2020-09-22       Impact factor: 5.922

4.  Phospholipid-Dependence of Plant UDP-Glucose Sterol beta-d-Glucosyl Transferase : IV. Reconstitution into Small Unilamellar Vesicles.

Authors:  A Ury; P Benveniste; P Bouvier-Navé
Journal:  Plant Physiol       Date:  1989-10       Impact factor: 8.340

5.  Separation of Escherichia coli penicillin-binding proteins into different membrane vesicles by agarose electrophoresis and sizing chromatography.

Authors:  M J Leidenix; G H Jacoby; T A Henderson; K D Young
Journal:  J Bacteriol       Date:  1989-10       Impact factor: 3.490

6.  Incorporation of hormone-sensitive lipase into phosphatidylcholine vesicles.

Authors:  C Holm; G Fredrikson; R Sundler; P Belfrage
Journal:  Lipids       Date:  1990-05       Impact factor: 1.880

7.  Determination of the size distribution of liposomes by SEC fractionation, and PCS analysis and enzymatic assay of lipid content.

Authors:  Lars Ingebrigtsen; Martin Brandl
Journal:  AAPS PharmSciTech       Date:  2002       Impact factor: 3.246

8.  Electrophysiological study with oxonol VI of passive NO3- transport by isolated plant root plasma membrane.

Authors:  P Pouliquin; J Grouzis; R Gibrat
Journal:  Biophys J       Date:  1999-01       Impact factor: 4.033

9.  Substructure of human von Willebrand factor.

Authors:  W E Fowler; L J Fretto; K K Hamilton; H P Erickson; P A McKee
Journal:  J Clin Invest       Date:  1985-10       Impact factor: 14.808

10.  Physiochemical characterization of the nisin-membrane interaction with liposomes derived from Listeria monocytogenes.

Authors:  K Winkowski; R D Ludescher; T J Montville
Journal:  Appl Environ Microbiol       Date:  1996-02       Impact factor: 4.792
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