Literature DB >> 6866769

The incorporation of O6-methyldeoxyguanosine and O4-methyldeoxythymidine monophosphates into DNA by DNA polymerases I and alpha.

J A Hall, R Saffhill.   

Abstract

The modified nucleoside 5'-triphosphates O6-MedGTP ad O4-MedTTP have been synthesised and their acceptability as DNA-precursors investigated using DNA polymerases I and alpha in an in vitro assay. O6-MedGMP is only incorporated into newly-synthesized DNA-like material in the presence of templates containing thymine bases. Similarly O4-MedTMP is only incorporated in the presence of templates containing guanine bases. The results confirm the promutagenic nature and base-pairing properties of O6-MeG ad O4-MeT.

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Year:  1983        PMID： 6866769      PMCID： PMC326033          DOI： 10.1093/nar/11.12.4185

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  21 in total

1.  D(M6ATP) as a probe of the fidelity of base incorporation into polynucleotides by Escherichia coli DNA polymerase I.

Authors:  J D Engel; P H von Hippel
Journal:  J Biol Chem       Date:  1978-02-10       Impact factor: 5.157

2.  Synthesis and chemical properties of monomers and polymers containing 7-methylguanine and an investigation of their substrate or template properties for bacterial deoxyribonucleic acid or ribonucleic acid polymerases.

Authors:  S Hendler; E Fürer; P R Srinivasan
Journal:  Biochemistry       Date:  1970-10-13       Impact factor: 3.162

3.  The properties of O 6 -methylguanine in templates for RNA polymerase.

Authors:  L L Gerchman; D B Ludlum
Journal:  Biochim Biophys Acta       Date:  1973-05-10

4.  Possible relevance of O-6 alkylation of deoxyguanosine to the mutagenicity and carcinogenicity of nitrosamines and nitrosamides.

Authors:  A Loveless
Journal:  Nature       Date:  1969-07-12       Impact factor: 49.962

5.  Comparative utilization of bromodeoxyuridine and iododeoxyuridine triphosphates for mammalian DNA replication in vitro.

Authors:  J R Gautschi; M Burkhalter; E A Baumann
Journal:  Biochim Biophys Acta       Date:  1978-03-29

6.  DNA-synthesis with methylated poly(dA-dT) templates: possible role of O4-methylthymine as a pro-mutagenic base.

Authors:  P J Abbott; R Saffhill
Journal:  Nucleic Acids Res       Date:  1977-03       Impact factor: 16.971

7.  Modified polynucleotides. I. Investigation of the enzymatic polymerization of 5-alkyl-dUTP-s.

Authors:  J T Sági; A Szabolcs; A Szemzö; L Otvös
Journal:  Nucleic Acids Res       Date:  1977-08       Impact factor: 16.971

8.  Preparation and template activities of polynucleotides containing O2- and O4-alkyluridine.

Authors:  B Singer; H Fraenkel-Conrat; J T Kuśmierek
Journal:  Proc Natl Acad Sci U S A       Date:  1978-04       Impact factor: 11.205

9.  Cellular reactions of O6-methylguanine, a product of some alkylating carcinogens.

Authors:  C T Miller; P D Lawley; S A Shah
Journal:  Biochem J       Date:  1973-10       Impact factor: 3.857

10.  Reaction products from N-methyl-N-nitrosourea and deoxyribonucleic acid containing thymidine residues. Synthesis and identification of a new methylation product, O4-methylthymidine.

Authors:  P D Lawley; D J Orr; S A Shah; P B Farmer; M Jarman
Journal:  Biochem J       Date:  1973-09       Impact factor: 3.857
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  9 in total

1.  Induction of mutation in mouse FM3A cells by N4-aminocytidine-mediated replicational errors.

Authors:  M Takahashi; M Nishizawa; K Negishi; F Hanaoka; M A Yamada; H Hayatsu
Journal:  Mol Cell Biol       Date:  1988-01       Impact factor: 4.272

2.  Effect of the direction of DNA replication on mutagenesis by N-methyl-N'-nitro-N-nitrosoguanidine in adapted cells of Escherichia coli.

Authors:  J Reed; F Hutchinson
Journal:  Mol Gen Genet       Date:  1987-07

3.  DNA sequence analysis of mutagenicity and site specificity of ethyl methanesulfonate in Uvr+ and UvrB- strains of Escherichia coli.

Authors:  P A Burns; F L Allen; B W Glickman
Journal:  Genetics       Date:  1986-08       Impact factor: 4.562

4.  A non-surgical method for induction of lung cancer in Wistar rats using a combination of NNK and high dietary fats.

Authors:  Shilpa Bhatnagar; Naveen Chaudhary; Deepshikha Pande Katare; S K Jain
Journal:  Protoplasma       Date:  2013-01-13       Impact factor: 3.356

5.  Possible depletion of a DNA repair enzyme in human lymphoma cells by subversive repair.

Authors:  P Karran
Journal:  Proc Natl Acad Sci U S A       Date:  1985-08       Impact factor: 11.205

6.  Mutagenic potential of O4-methylthymine in vivo determined by an enzymatic approach to site-specific mutagenesis.

Authors:  B D Preston; B Singer; L A Loeb
Journal:  Proc Natl Acad Sci U S A       Date:  1986-11       Impact factor: 11.205

7.  O4-Methyl, -ethyl, or -isopropyl substituents on thymidine in poly(dA-dT) all lead to transitions upon replication.

Authors:  B Singer; S J Spengler; H Fraenkel-Conrat; J T Kuśmierek
Journal:  Proc Natl Acad Sci U S A       Date:  1986-01       Impact factor: 11.205

8.  Active site and complete sequence of the suicidal methyltransferase that counters alkylation mutagenesis.

Authors:  B Demple; B Sedgwick; P Robins; N Totty; M D Waterfield; T Lindahl
Journal:  Proc Natl Acad Sci U S A       Date:  1985-05       Impact factor: 11.205

9.  Inducible repair of O-alkylated DNA pyrimidines in Escherichia coli.

Authors:  T V McCarthy; P Karran; T Lindahl
Journal:  EMBO J       Date:  1984-03       Impact factor: 11.598
  9 in total

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