Literature DB >> 6864218

Acetylcholine and choline in neuronal tissue measured by HPLC with electrochemical detection.

P E Potter, J L Meek, N H Neff.   

Abstract

A simple, rapid method is presented for the determination of acetylcholine (ACh) and choline (Ch) in neuronal tissue using HPLC with electrochemical detection. The method is based on the separation of ACh and Ch by reverse-phase HPLC and mixing the effluent as it emerges from the column with acetylcholinesterase and Ch oxidase, which converts endogenous Ch and Ch produced by the hydrolysis of ACh to betaine and hydrogen peroxide. Production of hydrogen peroxide is continuously monitored electrochemically. The sensitivity of the procedure is 1 pmol for Ch and 2 pmol for ACh. Specificity of the method is based on HPLC, two specific enzymatic reactions, and the detection of hydrogen peroxide.

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Year:  1983        PMID： 6864218     DOI： 10.1111/j.1471-4159.1983.tb13668.x

Source DB:  PubMed          Journal:  J Neurochem        ISSN： 0022-3042            Impact factor:   5.372


  30 in total

1.  Differences in the prejunctional effects of methacholine and pilocarpine on the release of endogenous acetylcholine from guinea-pig trachea.

Authors:  R E ten Berge; E C Weening; A F Roffel; J Zaagsma
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2.  Efficient measurement of endogenous neurotransmitters in small localized regions of central nervous systems in vitro with HPLC.

Authors:  Xuesi M Shao; Jack L Feldman
Journal:  J Neurosci Methods       Date:  2006-11-07       Impact factor: 2.390

3.  Activation of TrkA by nerve growth factor upregulates expression of the cholinergic gene locus but attenuates the response to ciliary neurotrophic growth factor.

Authors:  B Berse; I Lopez-Coviella; J K Blusztajn
Journal:  Biochem J       Date:  1999-09-01       Impact factor: 3.857

4.  Cerebrospinal fluid acetylcholine and choline in vascular dementia of Binswanger and multiple small infarct types as compared with Alzheimer-type dementia.

Authors:  H Tohgi; T Abe; M Kimura; M Saheki; S Takahashi
Journal:  J Neural Transm (Vienna)       Date:  1996       Impact factor: 3.575

5.  Isolation and enzymic assay of choline and phosphocholine present in cell extracts with picomole sensitivity.

Authors:  J J Murray; T T Dinh; A P Truett; D A Kennerly
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6.  Effects of the centrally acting cholinesterase inhibitors tetrahydroaminoacridine and E2020 on the basal concentration of extracellular acetylcholine in the hippocampus of freely moving rats.

Authors:  K Kawashima; A Sato; M Yoshizawa; T Fujii; K Fujimoto; T Suzuki
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  1994-11       Impact factor: 3.000

7.  Trans-synaptic stimulation of cortical acetylcholine release after partial 192 IgG-saporin-induced loss of cortical cholinergic afferents.

Authors:  J Fadel; H Moore; M Sarter; J P Bruno
Journal:  J Neurosci       Date:  1996-10-15       Impact factor: 6.167

8.  Phospholipase D-catalyzed hydrolysis of phosphatidylcholine provides the choline precursor for acetylcholine synthesis in a human neuronal cell line.

Authors:  H C Lee; M P Fellenz-Maloney; M Liscovitch; J K Blusztajn
Journal:  Proc Natl Acad Sci U S A       Date:  1993-11-01       Impact factor: 11.205

9.  Sources of adenosine released during neuromuscular transmission in the rat.

Authors:  D O Smith
Journal:  J Physiol       Date:  1991-01       Impact factor: 5.182

10.  Disruption of mesolimbic regulation of prefrontal cholinergic transmission in an animal model of schizophrenia and normalization by chronic clozapine treatment.

Authors:  Kathleen S Alexander; Julie M Brooks; Martin Sarter; John P Bruno
Journal:  Neuropsychopharmacology       Date:  2009-08-19       Impact factor: 7.853
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