Literature DB >> 6856477

Conformational analysis of a ribopentanucleoside tetraphosphate in aqueous solution. A two-dimensional NMR study at 500 MHz.

P P Lankhorst, G Wille, J H van Boom, C Altona, C A Haasnoot.   

Abstract

The 30 ribose proton resonances of the pentaribonucleoside tetraphosphate m6(2)AUm6(2)AUm6(2)A have been assigned unequivocally by means of spin-echo-correlated spectroscopy, 2D J-resolved spectroscopy and Nuclear Overhauser difference spectroscopy, carried out at 500 MHz. A detailed comparison of the conformational properties of the title compound with its constituent fragments m6(2)AUm6(2)AU, m6(2)AUm6(2)A, m6(2)AU and the relevant monomers is given. Chemical shift data indicate the existence of a doubly "bulged out" conformer, in which the two interior U-fragments are not involved in regular nearest neighbour stacking interactions. The coupling constants of the ribose-ring are interpreted in terms of the N/S equilibrium, and population distributions along the backbone angles beta and gamma are presented. The combined data suggest a strong similarity between the 5'-terminal triplets in m6(2)AUm6(2)AUm6(2)A, m6(2)AUm6(2)AU and m6(2)AUm6(2)A2.

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Year:  1983        PMID： 6856477      PMCID： PMC325927          DOI： 10.1093/nar/11.9.2839

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  14 in total

1.  Two-dimensional J-resolved 1H n.m.r. spectroscopy for studies of biological macromolecules.

Authors:  K Nagayama; K Wüthrich; P Bachmann; R R Ernst
Journal:  Biochem Biophys Res Commun       Date:  1977-09-09       Impact factor: 3.575

2.  Conformational analysis of the sugar ring in nucleosides and nucleotides. Improved method for the interpretation of proton magnetic resonance coupling constants.

Authors:  C Altona; M Sundaralingam
Journal:  J Am Chem Soc       Date:  1973-04-04       Impact factor: 15.419

3.  Conformation of the exocyclic 5'-CH 2 OH in nucleosides and nucleotides in aqueous solution from specific assignments of the H 5' and H 5'' signals in the NMR spectra.

Authors:  M Remin; D Shugar
Journal:  Biochem Biophys Res Commun       Date:  1972-08-07       Impact factor: 3.575

4.  The RNA primer promoter, as defined in vitro, is essential for pMB1 plasmid replication in vivo.

Authors:  G Cesareni
Journal:  J Mol Biol       Date:  1982-09       Impact factor: 5.469

5.  Influence of the 2'-hydroxyl group and of 6-N-methylation on the conformation of adenine dinucleoside monophosphates in solution. A nuclear magnetic resonance and circular dichroism study.

Authors:  C S Olsthoorn; J Doornbos; H P de Leeuw; C Altona
Journal:  Eur J Biochem       Date:  1982-07

6.  Molecular-mechanical studies of Z-DNA: a comparison of the structural and energetic properties of Z- and B-DNA.

Authors:  P Kollman; P Weiner; G Quigley; A Wang
Journal:  Biopolymers       Date:  1982-10       Impact factor: 2.505

7.  Two-dimensional spin echo correlated spectroscopy (SECSY) for 1H NMR studies of biological macromolecules.

Authors:  K Nagayama; K Wüthrich; R R Ernst
Journal:  Biochem Biophys Res Commun       Date:  1979-09-12       Impact factor: 3.575

8.  Conformation studies of 13 trinucleoside diphosphates by 360 MHz PMR spectroscopy. A bulged base conformation. I. Base protons and H1' protons.

Authors:  C H Lee; I Tinoco
Journal:  Biophys Chem       Date:  1980-04       Impact factor: 2.352

9.  Thermodynamics of stacking and of self-association of the dinucleoside monophosphate m2(6)A-U from proton NMR chemical shifts: differential concentration temperature profile method.

Authors:  A J Hartel; P P Lankhorst; C Altona
Journal:  Eur J Biochem       Date:  1982-12-15

10.  Polynucleotides. XIV. Photochemical evidence for an extrahelical, solvent-accessible environment of non-complementary residues in polynucleotide helices.

Authors:  A J Lomant; J R Fresco
Journal:  J Mol Biol       Date:  1973-07-05       Impact factor: 5.469
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  4 in total

1.  Bulge-out structures in the single-stranded trimer AUA and in the duplex (CUGGUGCGG).(CCGCCCAG). A model-building and NMR study.

Authors:  Y T van den Hoogen; A A van Beuzekom; E de Vroom; G A van der Marel; J H van Boom; C Altona
Journal:  Nucleic Acids Res       Date:  1988-06-10       Impact factor: 16.971

2.  Conformational characteristics of the hexanucleoside pentaphosphate AUAUAU: a 2D NMR study at 500 MHz.

Authors:  P P Lankhorst; G A van der Marel; G Wille; J H van Boom; C Altona
Journal:  Nucleic Acids Res       Date:  1985-05-10       Impact factor: 16.971

3.  Nuclear magnetic resonance study of d-TGGCCA in solution.

Authors:  E Guittet; D Piveteau; J Y Lallemand; T Huyn-Dinh; J Igolen
Journal:  Nucleic Acids Res       Date:  1984-07-25       Impact factor: 16.971

4.  Synthesis, complete 1H assignments and conformations of the self-complementary hexadeoxyribonucleotide [d(CpGpApTpCpG)]2 and its fragments by high field NMR.

Authors:  J W Lown; C C Hanstock; R C Bleackley; J L Imbach; B Rayner; J J Vasseur
Journal:  Nucleic Acids Res       Date:  1984-03-12       Impact factor: 16.971
  4 in total

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