Literature DB >> 6462922

Nuclear magnetic resonance study of d-TGGCCA in solution.

E Guittet, D Piveteau, J Y Lallemand, T Huyn-Dinh, J Igolen.   

Abstract

The 1H NMR spectrum of a self complementary deoxyhexanucleotide (d-TGGCCA) in water solution has been completely and unambiguously assigned by use of two-dimensional techniques and phosphorus off-resonance decoupling experiments. The helix-coil transition has been monitored by following most of the non-exchangeable protons against temperature and was shown to be a cooperative dissociation-association process. Sequential fraying is sizable even at low temperature. Considerations of coupling constants and of Nuclear Overhauser effects show the helix to be of the B-DNA type.

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Year:  1984        PMID: 6462922      PMCID: PMC320041          DOI: 10.1093/nar/12.14.5927

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN: 0305-1048            Impact factor:   16.971


  14 in total

1.  Improved triester approach for the synthesis of pentadecathymidylic acid.

Authors:  K Itakura; N Katagiri; C P Bahl; R H Wightman; S A Narang
Journal:  J Am Chem Soc       Date:  1975-12-10       Impact factor: 15.419

2.  Conformation of the exocyclic 5'-CH 2 OH in nucleosides and nucleotides in aqueous solution from specific assignments of the H 5' and H 5'' signals in the NMR spectra.

Authors:  M Remin; D Shugar
Journal:  Biochem Biophys Res Commun       Date:  1972-08-07       Impact factor: 3.575

3.  Assignment of the non-exchangeable proton resonances of d(C-G-C-G-A-A-T-T-C-G-C-G) using two-dimensional nuclear magnetic resonance methods.

Authors:  D R Hare; D E Wemmer; S H Chou; G Drobny; B R Reid
Journal:  J Mol Biol       Date:  1983-12-15       Impact factor: 5.469

4.  Nuclear overhauser studies of CCGGAp, ACCGGp, and ACCGGUp.

Authors:  M Petersheim; D H Turner
Journal:  Biochemistry       Date:  1983-01-18       Impact factor: 3.162

5.  Proton magnetic resonance melting studies of CCGGp, CCGGAp, ACCGGp, CCGGUp, and ACCGGUp.

Authors:  M Petersheim; D H Turner
Journal:  Biochemistry       Date:  1983-01-18       Impact factor: 3.162

6.  Premelting and melting transitions in the d(CGCGAATTCGCG) self-complementary duplex in solution.

Authors:  D J Patel; S A Kozlowski; L A Marky; C Broka; J A Rice; K Itakura; K J Breslauer
Journal:  Biochemistry       Date:  1982-02-02       Impact factor: 3.162

7.  Two-dimensional spin echo correlated spectroscopy (SECSY) for 1H NMR studies of biological macromolecules.

Authors:  K Nagayama; K Wüthrich; R R Ernst
Journal:  Biochem Biophys Res Commun       Date:  1979-09-12       Impact factor: 3.575

8.  Right-handed and left-handed DNA: studies of B- and Z-DNA by using proton nuclear Overhauser effect and P NMR.

Authors:  D J Patel; S A Kozlowski; A Nordheim; A Rich
Journal:  Proc Natl Acad Sci U S A       Date:  1982-03       Impact factor: 11.205

9.  Specific platinum chelation by the guanines of the deoxyhexanucleotide d(T-G-G-C-C-A) upon reaction with cis-[Pt(NH3)2(H2O)2](NO3)2.

Authors:  J P Girault; J C Chottard; E R Guittet; J Y Lallemand; T Huynh-Dinh; J Igolen
Journal:  Biochem Biophys Res Commun       Date:  1982-12-31       Impact factor: 3.575

10.  DNA fragment conformations IV - Helix-coil transition and conformation of d-CCATGG in aqueous solution by 1H-NMR spectroscopy.

Authors:  S Tran-Dinh; J M Neumann; T Huynh-Dinh; P Allard; J Y Lallemand; J Igolen
Journal:  Nucleic Acids Res       Date:  1982-09-11       Impact factor: 16.971

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  3 in total

1.  Study of structure, base-pair opening kinetics and proton exchange mechanism of the d-(AATTGCAATT) self-complementary oligodeoxynucleotide in solution.

Authors:  M Kochoyan; G Lancelot; J L Leroy
Journal:  Nucleic Acids Res       Date:  1988-08-11       Impact factor: 16.971

2.  2D-NMR studies of the unnatural duplex alpha-d(TCTAAAC)-beta-d(AGATTTG).

Authors:  G Lancelot; J L Guesnet; V Roig; N T Thuong
Journal:  Nucleic Acids Res       Date:  1987-09-25       Impact factor: 16.971

3.  alpha-DNA-III. Characterization by high field 1H-NMR, anti-parallel self-recognition and conformation of the unnatural hexadeoxyribonucleotides alpha-[d(CpApTpGpCpG)] and alpha-[d(CpGpCpApTpG)]. Alpha-oligodeoxynucleotides as potential cellular probes for gene control.

Authors:  F Morvan; B Rayner; J L Imbach; D K Chang; J W Lown
Journal:  Nucleic Acids Res       Date:  1987-05-26       Impact factor: 16.971

  3 in total

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