Literature DB >> 6855484

Determination of lycopene, alpha- and beta-carotene and retinyl esters in human serum by reversed-phase high performance liquid chromatography.

C R Broich, L E Gerber, J W Erdman.   

Abstract

A rapid specific, microdetermination of the major human blood carotenoids by high performance liquid chromatography (HPLC) separation and quantitation at 466 nm is detailed in this paper. Serum retinyl esters can also be quantified utilizing the same separation procedure but detected at 325 nm. One hundred microliters of deproteinated serum were extracted with chloroform and injected on a reverse-phase column. Separation occurred within 16 min for all compounds of interest employing a mobile solvent of MeOH/AcN/CHCl3 (47:47:6). All compounds were quantified at the wavelengths cited by integrated peak areas using retinyl acetate as a daily standard. Analysis of serum from a hypercarotenemic anorexia nervosa patient and a person suffering from hypervitaminosis A are presented as examples of the clinical application of this procedure.

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Year:  1983        PMID： 6855484     DOI： 10.1007/bf02534557

Source DB:  PubMed          Journal:  Lipids        ISSN： 0024-4201            Impact factor:   1.880


  14 in total

Review 1.  Microdetermination of vitamin A and carotenoids in blood and tissue.

Authors:  D S McLaren; W W Read; Z L Awdeh; M Tchalian
Journal:  Methods Biochem Anal       Date:  1967

2.  Fractionation and quantification of beta carotene and vitamin A derivatives in human serum.

Authors:  S R Targan; S Merrill; A D Schwabe
Journal:  Clin Chem       Date:  1969-06       Impact factor: 8.327

3.  Protracted hypervitaminosis A following long-term, low-level intake.

Authors:  W A Farris; J W Erdman
Journal:  JAMA       Date:  1982-03-05       Impact factor: 56.272

4.  Separation of long-chain fatty acid esters of retinol by high-performance liquid chromatography.

Authors:  A C Ross
Journal:  Anal Biochem       Date:  1981-08       Impact factor: 3.365

5.  High-performance liquid chromatography of 13-cis-retinoic acid and of endogenous retinol in human plasma.

Authors:  J G Besner; R Leclaire
Journal:  J Chromatogr       Date:  1980-09-12

6.  High-pressure liquid chromatographic resolution of vitamin A compounds.

Authors:  A M McCormick; J L Napoli; H F Deluca
Journal:  Anal Biochem       Date:  1978-05       Impact factor: 3.365

7.  Metabolism of retinol-binding protein and vitamin A during hypervitaminosis A in the rat.

Authors:  A K Mallia; J E Smith; D W Goodman
Journal:  J Lipid Res       Date:  1975-05       Impact factor: 5.922

8.  Simultaneous determination of alpha-tocopherol and retinol in plasma or red cells by high pressure liquid chromatography.

Authors:  J G Bieri; T J Tolliver; G L Catignani
Journal:  Am J Clin Nutr       Date:  1979-10       Impact factor: 7.045

9.  An evaluation of trace metals, vitamins, and taste function in anorexia nervosa.

Authors:  R C Casper; B Kirschner; H H Sandstead; R A Jacob; J M Davis
Journal:  Am J Clin Nutr       Date:  1980-08       Impact factor: 7.045

10.  High pressure liquid chromatographic determination of vitamins A and E in cereal products.

Authors:  W A Widicus; J R Kirk
Journal:  J Assoc Off Anal Chem       Date:  1979-05
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  4 in total

1.  Clinical trial of lutein in patients with retinitis pigmentosa receiving vitamin A.

Authors:  Eliot L Berson; Bernard Rosner; Michael A Sandberg; Carol Weigel-DiFranco; Robert J Brockhurst; K C Hayes; Elizabeth J Johnson; Ellen J Anderson; Chris A Johnson; Alexander R Gaudio; Walter C Willett; Ernst J Schaefer
Journal:  Arch Ophthalmol       Date:  2010-04

2.  Screening and selection of high carotenoid producing in vitro tomato cell culture lines for [13C]-carotenoid production.

Authors:  Nancy J Engelmann; Jessica K Campbell; Randy B Rogers; S Indumathie Rupassara; Peter J Garlick; Mary Ann Lila; John W Erdman
Journal:  J Agric Food Chem       Date:  2010-09-22       Impact factor: 5.279

3.  Reversed phase HPLC analysis of alpha- and beta-carotene from selected raw and cooked vegetables.

Authors:  J M Dietz; S Sri Kantha; J W Erdman
Journal:  Plant Foods Hum Nutr       Date:  1988       Impact factor: 3.921

4.  Effect of vitamin A supplementation on rhodopsin mutants threonine-17 --> methionine and proline-347 --> serine in transgenic mice and in cell cultures.

Authors:  T Li; M A Sandberg; B S Pawlyk; B Rosner; K C Hayes; T P Dryja; E L Berson
Journal:  Proc Natl Acad Sci U S A       Date:  1998-09-29       Impact factor: 11.205
  4 in total

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