Literature DB >> 6853734

A fluorimetric method for red blood cell sorbitol dehydrogenase activity.

G Vaca, P Zúñiga, C Medina, R Alonso, G González-Quiroga, R I Ortíz-De-Luna, J M Cantú.   

Abstract

A new fluorimetric method for the quantification of red blood cell (RBC) sorbitol dehydrogenase is described. It is based on the oxidation of sorbitol to fructose, in presence of NAD+, catalysed by the RBC-sorbitol dehydrogenase. The quantity of NADH formed is then measured in a filter fluorimeter. Comparison with an indirect spectrophotometric assay yielded good correlation; however, the present method offers several advantages: it is more rapid, simple and inexpensive. It should be useful to screen for sorbitol dehydrogenase deficiency in large numbers of individuals, particularly patients with diabetes or cataracts.

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Year:  1983        PMID： 6853734      PMCID： PMC498353          DOI： 10.1136/jcp.36.6.697

Source DB:  PubMed          Journal:  J Clin Pathol        ISSN： 0021-9746            Impact factor:   3.411


  17 in total

1.  The role of the polyol pathway in methaemoglobin reduction in human red cells.

Authors:  S F Travis; A D Morrison; R S Clements; A I Winegrad; F A Oski
Journal:  Br J Haematol       Date:  1974-08       Impact factor: 6.998

2.  [Demonstration of sorbite dehydrogenase in human erythrocytes].

Authors:  B Morsches; H Holzmann; C Bettingen
Journal:  Klin Wochenschr       Date:  1969-06-15

Review 3.  The sorbitol pathway and the complications of diabetes.

Authors:  K H Gabbay
Journal:  N Engl J Med       Date:  1973-04-19       Impact factor: 91.245

4.  The role of fructose in restoration of organic phosphate compounds in outdated bank blood.

Authors:  J D Torrance
Journal:  J Lab Clin Med       Date:  1973-09

5.  Starch-gel electrophoresis of four enzymes from human red blood cells: glyceraldehyde-3-phosphate dehydrogenase, fructoaldolase, glyoxalase II and sorbitol dehydrogenase.

Authors:  D Charlesworth
Journal:  Ann Hum Genet       Date:  1972-04       Impact factor: 1.670

6.  New rapid method for the estimation of red cell galactose-1-phosphate uridyl transferase activity.

Authors:  E Beutler; M Mitchell
Journal:  J Lab Clin Med       Date:  1968-09

7.  Glucose utilization by the polyol pathway in human erythrocytes.

Authors:  A D Morrison; R S Clements; S B Travis; F Oski; A I Winegrad
Journal:  Biochem Biophys Res Commun       Date:  1970-07-13       Impact factor: 3.575

8.  Metabolic alterations in the human erythrocyte produced by increases in glucose concentration. The role of the polyol pathway.

Authors:  S F Travis; A D Morrison; R S Clements; A I Winegrad; F A Oski
Journal:  J Clin Invest       Date:  1971-10       Impact factor: 14.808

9.  The reduction of glyceraldehyde by human erythrocytes. L-hexonate dehydrogenase activity.

Authors:  E Beutler; E Guinto
Journal:  J Clin Invest       Date:  1974-05       Impact factor: 14.808

10.  Sorbitol pathway: presence in nerve and cord with substrate accumulation in diabetes.

Authors:  K H Gabbay; L O Merola; R A Field
Journal:  Science       Date:  1966-01-14       Impact factor: 47.728
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  2 in total

1.  Sorbitol dehydrogenase deficiency in several pig tissues: potential implications for studies of experimental diabetes.

Authors:  G Vaca; R Alonso; P Zúñiga; G González-Quiroga; M A Chávez; C Medina; C Wunsch; J M Cantú
Journal:  Diabetologia       Date:  1984-10       Impact factor: 10.122

2.  Membrane-bound sorbitol dehydrogenase in human red blood cells. Studies in normal subjects and in enzyme-deficient subjects with congenital cataracts.

Authors:  A Alvarez; A Martínez; B Ibarra; C Medina; M Bracamontes; J Perea; G Vaca
Journal:  J Inherit Metab Dis       Date:  1993       Impact factor: 4.982
  2 in total

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