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Literature DB >> 6847615

Ca2+-dependent high-affinity complex formation between calmodulin and melittin.

Abstract

The amphiphatic polypeptide melittin migrates as an equimolar complex with bovine brain calmodulin when monitored by gel disc electrophoresis or gel filtration in the presence of Ca2+, even in 4M-urea. The complex disassociates in the presence of EDTA and urea. The affinity is of the same order as that of calmodulin for its target enzymes, and more than 1000-fold higher than that of calmodulin for basic peptide hormones or hydrophobic drugs. The activation of brain phosphodiesterase by calmodulin is inhibited by melittin. The kinetics of inhibition suggest competition between the enzyme and melittin for calmodulin. The calmodulin-melittin interaction may constitute a model for that existing between calmodulin and its target enzymes.

Entities: Chemical Gene Species

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Year: 1983 PMID： 6847615 PMCID： PMC1154084 DOI： 10.1042/bj2090269

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

27 in total

1. Control of microtubule assembly-disassembly by calcium-dependent regulator protein.

Authors: J M Marcum; J R Dedman; B R Brinkley; A R Means
Journal: Proc Natl Acad Sci U S A Date: 1978-08 Impact factor: 11.205

2. A modified assay of 3':5'-cyclic-AMP phosphodiesterase.

Authors: R J Boudreau; G I Drummond
Journal: Anal Biochem Date: 1975-02 Impact factor: 3.365

3. Spectroscopic determination of tryptophan and tyrosine in proteins.

Authors: H Edelhoch
Journal: Biochemistry Date: 1967-07 Impact factor: 3.162

4. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors: M M Bradford
Journal: Anal Biochem Date: 1976-05-07 Impact factor: 3.365

5. The interaction of the calcium-binding protein (troponin C) with bivalent cations and the inhibitory protein (troponin I).

Authors: J F Head; S V Perry
Journal: Biochem J Date: 1974-02 Impact factor: 3.857

6. Two species of full-length cDNA are synthesized in high yield by melittin-treated avian retrovirus particles.

Authors: L R Boone; A Skalka
Journal: Proc Natl Acad Sci U S A Date: 1980-02 Impact factor: 11.205

7. Affinity-chromatographic isolation and some properties of troponin C from different muscle types.

Authors: J F Head; R A Weeks; S V Perry
Journal: Biochem J Date: 1977-03-01 Impact factor: 3.857

8. High-resolution 1H-NMR studies of self-aggregation of melittin in aqueous solution.

Authors: L R Brown; J Lauterwein; K Wüthrich
Journal: Biochim Biophys Acta Date: 1980-04-25

9. Interaction of beta-endorphin and other opioid peptides with calmodulin.

Authors: M Sellinger-Barnette; B Weiss
Journal: Mol Pharmacol Date: 1982-01 Impact factor: 4.436

10. Structural similarities between the Ca2+-dependent regulatory proteins of 3':5'-cyclic nucleotide phosphodiesterase and actomyosin ATPase.

Authors: D M Watterson; W G Harrelson; P M Keller; F Sharief; T C Vanaman
Journal: J Biol Chem Date: 1976-08-10 Impact factor: 5.157

26 in total

Ca2+-dependent high-affinity complex formation between calmodulin and melittin.

1. Control of microtubule assembly-disassembly by calcium-dependent regulator protein.

2. A modified assay of 3':5'-cyclic-AMP phosphodiesterase.

3. Spectroscopic determination of tryptophan and tyrosine in proteins.

4. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

5. The interaction of the calcium-binding protein (troponin C) with bivalent cations and the inhibitory protein (troponin I).

6. Two species of full-length cDNA are synthesized in high yield by melittin-treated avian retrovirus particles.

7. Affinity-chromatographic isolation and some properties of troponin C from different muscle types.

8. High-resolution 1H-NMR studies of self-aggregation of melittin in aqueous solution.

9. Interaction of beta-endorphin and other opioid peptides with calmodulin.

10. Structural similarities between the Ca2+-dependent regulatory proteins of 3':5'-cyclic nucleotide phosphodiesterase and actomyosin ATPase.

1. Conformational and thermodynamic properties of peptide binding to the human S100P protein.

2. Investigation of calmodulin-Peptide interactions using matrix-assisted laser desorption/ionization mass spectrometry.

3. Melittin binding causes a large calcium-dependent conformational change in calmodulin.

Review 4. Nox Inhibitors & Therapies: Rational Design of Peptidic and Small Molecule Inhibitors.

5. High-affinity formation of a 2:1 complex between gramicidin S and calmodulin.

6. The cilia of Paramecium tetraurelia contain both Ca2+-dependent and Ca2+-inhibitable calmodulin-binding proteins.

7. Affinity purification of seminalplasmin and characterization of its interaction with calmodulin.

Review 8. Interactive properties of calmodulin.

9. Hydrophobic Peptides Affect Binding of Calmodulin and Ca as Explored by H/D Amide Exchange and Mass Spectrometry.

10. Asymmetry of calmodulin revealed by peptide binding.