Literature DB >> 6846837

Reverse-phase high-performance liquid chromatography of chemically modified DNA.

J D Gomes, C J Chang.   

Abstract

A reverse-phase high-performance liquid chromatographic method has been developed to determine the sites of reaction and the product distribution of modified salmon sperm DNA. The DNA was reacted with methyl methanesulfonate in neutral solution, and then degraded into deoxyribonucleosides by snake venom phosphodiesterase and alkaline phosphatase. Four products were identified and quantitated: 7-methyldeoxyguanosine (37.1%), 7-methylguanine (7.3%), 3-methyldeoxycytidine (28.8%), and 1-methyldeoxyadenosine (26.8%). This method provides a rapid procedure for analysis of chemically or biochemically modified nucleic acids.

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Year:  1983        PMID： 6846837     DOI： 10.1016/0003-2697(83)90566-3

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  7 in total

Review 1.  Quantitative assessment of DNA methylation: Potential applications for disease diagnosis, classification, and prognosis in clinical settings.

Authors:  Romulo Martin Brena; Tim Hui-Ming Huang; Christoph Plass
Journal:  J Mol Med (Berl)       Date:  2006-01-17       Impact factor: 4.599

Review 2.  Chemical biology of mutagenesis and DNA repair: cellular responses to DNA alkylation.

Authors:  Nidhi Shrivastav; Deyu Li; John M Essigmann
Journal:  Carcinogenesis       Date:  2009-10-29       Impact factor: 4.944

3.  Fused-core silica column ultra-performance liquid chromatography-ion trap tandem mass spectrometry for determination of global DNA methylation status.

Authors:  Ill Yang; Marie C Fortin; Jason R Richardson; Brian Buckley
Journal:  Anal Biochem       Date:  2010-10-13       Impact factor: 3.365

4.  Mutagenesis, genotoxicity, and repair of 1-methyladenine, 3-alkylcytosines, 1-methylguanine, and 3-methylthymine in alkB Escherichia coli.

Authors:  James C Delaney; John M Essigmann
Journal:  Proc Natl Acad Sci U S A       Date:  2004-09-20       Impact factor: 11.205

5.  Repair of DNA Alkylation Damage by the Escherichia coli Adaptive Response Protein AlkB as Studied by ESI-TOF Mass Spectrometry.

Authors:  Deyu Li; James C Delaney; Charlotte M Page; Alvin S Chen; Cintyu Wong; Catherine L Drennan; John M Essigmann
Journal:  J Nucleic Acids       Date:  2010-10-27

6.  DNA methylation: a timeline of methods and applications.

Authors:  Alan Harrison; Anne Parle-McDermott
Journal:  Front Genet       Date:  2011-10-25       Impact factor: 4.599

7.  Characterization of Byproducts from Chemical Syntheses of Oligonucleotides Containing 1-Methyladenine and 3-Methylcytosine.

Authors:  Qi Tang; Ang Cai; Ke Bian; Fangyi Chen; James C Delaney; Sravani Adusumalli; Alvin C Bach; Fatemeh Akhlaghi; Bongsup P Cho; Deyu Li
Journal:  ACS Omega       Date:  2017-11-20
  7 in total

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