Genetic analysis of the T-H-2 region in non-t chromosomes. I. Two new congenic strains isolate Qglo-1 from H-2.

A general breeding protocol useful in the construction of congenic lines of mice disparate in the 15 cM T-H-2 region of chromosome 17 in non-t chromosomes is described. Two such congenic lines, B6.TC2/Rn and B6.TC3/Rn, were derived from the C57BL/6J and B6.C-H-2d/By strains using this protocol. Both B6.TC2 and B6.TC3 dissociate the quantitative activity locus for glyoxalase I (Qglo-1) from the H-2 complex, and hence possess BALB/cBy DNA centromeric to H-2. However, neither new strain is able to map any H-2-associated restriction fragment length polymorphism with an H-2 cDNA probe even though both strains are recombinant in the 2 cM Qglo-1-H-2K interval.