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Literature DB >> 6833387

Tropomyosin-like properties of clathrin light chains allow a rapid, high-yield purification.

Abstract

The light chains (LCa and LCb) of bovine brain clathrin are resistant to heat denaturation by boiling, a property shared by tropomyosin (Bailey, K., 1948, Biochem. J., 43:271-281). Light chains were partially purified by boiling and centrifugation of a Tris-extract of crude membranes prepared from bovine brains (Keen, J. H., M. C. Willingham, and I. H. Pastan, 1979, Cell., 16:303-312). Contaminant polypeptides were then removed by size-exclusion high-pressure liquid chromatography. The purified light chains were separated from each other by using an immunoaffinity column prepared from a monoclonal antibody CVC.7 specific for LCa and not LCb.

Entities: Chemical Gene Species

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Year: 1983 PMID： 6833387 PMCID： PMC2112400 DOI： 10.1083/jcb.96.3.911

Source DB: PubMed Journal: J Cell Biol ISSN： 0021-9525 Impact factor: 10.539

20 in total

1. A chemical comparison of tropomyosins from muscle and non-muscle tissues.

Authors: R E Fine; A L Blitz
Journal: J Mol Biol Date: 1975-07-05 Impact factor: 5.469

2. Coated vesicles from pig brain: purification and biochemical characterization.

Authors: B M Pearse
Journal: J Mol Biol Date: 1975-09-05 Impact factor: 5.469

3. Clathrin: a unique protein associated with intracellular transfer of membrane by coated vesicles.

Authors: B M Pearse
Journal: Proc Natl Acad Sci U S A Date: 1976-04 Impact factor: 11.205

4. On the structure of coated vesicles.

Authors: R A Crowther; J T Finch; B M Pearse
Journal: J Mol Biol Date: 1976-06-05 Impact factor: 5.469

5. Coated vesicles: characterization, selective dissociation, and reassembly.

Authors: M P Woodward; T F Roth
Journal: Proc Natl Acad Sci U S A Date: 1978-09 Impact factor: 11.205

6. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.

Authors: D W Cleveland; S G Fischer; M W Kirschner; U K Laemmli
Journal: J Biol Chem Date: 1977-02-10 Impact factor: 5.157

7. A simplified method for cyanogen bromide activation of agarose for affinity chromatography.

Authors: S C March; I Parikh; P Cuatrecasas
Journal: Anal Biochem Date: 1974-07 Impact factor: 3.365

8. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

9. Conditions for purification of anti-Brucella antibodies by immunoadsorption and elution.

Authors: R S Read; J C Cox; H A Ward; R C Nairn
Journal: Immunochemistry Date: 1974-12

10. Evidence that coated vesicles isolated from brain are calcium-sequestering organelles resembling sarcoplasmic reticulum.

Authors: A L Blitz; R E Fine; P A Toselli
Journal: J Cell Biol Date: 1977-10 Impact factor: 10.539

9 in total

Tropomyosin-like properties of clathrin light chains allow a rapid, high-yield purification.

1. A chemical comparison of tropomyosins from muscle and non-muscle tissues.

2. Coated vesicles from pig brain: purification and biochemical characterization.

3. Clathrin: a unique protein associated with intracellular transfer of membrane by coated vesicles.

4. On the structure of coated vesicles.

5. Coated vesicles: characterization, selective dissociation, and reassembly.

6. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.

7. A simplified method for cyanogen bromide activation of agarose for affinity chromatography.

8. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

9. Conditions for purification of anti-Brucella antibodies by immunoadsorption and elution.

10. Evidence that coated vesicles isolated from brain are calcium-sequestering organelles resembling sarcoplasmic reticulum.

1. Site-specific disruption of clathrin assembly produces novel structures.

2. Biochemical and immunological studies on clathrin light chains and their binding sites on clathrin triskelions.

3. Clathrin heavy chain, light chain interactions.

4. Comparison of the primary structures of clathrin light chains from bovine brain and adrenal gland by peptide mapping.

5. Dissociation of clathrin coats coupled to the hydrolysis of ATP: role of an uncoating ATPase.

6. Clathrin assembly involves a light chain-binding region.

7. Predominance of clathrin light chain LCb correlates with the presence of a regulated secretory pathway.

8. Clathrin structure characterized with monoclonal antibodies. I. Analysis of multiple antigenic sites.

9. Tubulin as a molecular component of coated vesicles.