A comparison of some of the properties of the [3H]-sulpiride binding site in soluble and native membrane preparations of dog striatum.

[3H]-sulpiride binding sites in dog striatum have been solubilised using CHAPS detergent. Solubilised sites were similar to those in native membrane preparations in terms of Bmax, Kd and sensitivity to dopamine and dopamine receptor antagonists. Binding of [3H]-sulpiride to the solubilised binding site was dependent upon the presence of sodium ions and was inactivated by the sulphydryl group reagent N-ethylmaleimide.