Literature DB >> 6809639

Quantitation of nasal secretory IgA by enzyme-linked immunosorbent assay.

Y Hamaguchi, M Ohi, Y Sakakura, T Mukojima.   

Abstract

A simple and sensitive technique, enzyme-linked immunosorbent assay (ELISA) was used for the quantitation of secretory IgA (sIgA) in order to study the synthesis and transport of sIgA in the nasal secretion from patients with chronic sinusitis. Rough-surface glass beads coated with antisecretory component antibody were employed as the solid phase. The minimal detectable concentration of sIgA was 50 ng/ml in this study. sIgA averaged 226.3 +/- 217.4 mg/dl and total IgA averaged 302.0 +/- 137.5 mg/dl.

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Year:  1982        PMID: 6809639     DOI: 10.1159/000233136

Source DB:  PubMed          Journal:  Int Arch Allergy Appl Immunol        ISSN: 0020-5915


  3 in total

1.  Immunoglobulins in nasal secretions of healthy humans: structural integrity of secretory immunoglobulin A1 (IgA1) and occurrence of neutralizing antibodies to IgA1 proteases of nasal bacteria.

Authors:  L Kirkeby; T T Rasmussen; J Reinholdt; M Kilian
Journal:  Clin Diagn Lab Immunol       Date:  2000-01

2.  A class capture enzyme immunoassay for immunoglobulin level determinations in bovine sera.

Authors:  K Nielsen; B Rosenbaum; J Stiller
Journal:  Can J Comp Med       Date:  1985-10

3.  Antibody assay of rabbit sera for outer membrane vesicles of Haemophilus influenzae by enzyme-linked immunosorbent assay.

Authors:  T Harada; Y Sakakura; Y Yoshinaka
Journal:  Med Microbiol Immunol       Date:  1985       Impact factor: 3.402

  3 in total

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