Literature DB >> 3877861

Antibody assay of rabbit sera for outer membrane vesicles of Haemophilus influenzae by enzyme-linked immunosorbent assay.

T Harada, Y Sakakura, Y Yoshinaka.   

Abstract

Enzyme-linked immunosorbent assay (ELISA) was applied to the detection of IgG and IgM antibodies against outer membrane vesicles (OMV) antigen of Haemophilus influenzae type b. In this ELISA system, IgG antibody titers were about 40 fold higher than those in indirect hemagglutination assay (IHA). The IgG antibody titers by this ELISA of rabbit sera obtained after immunization were comparable with those by radioimmunoassay (RIA) of the same sera. A significant correlation was established between these two assays (r = 0.973, P less than 0.001).

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Year:  1985        PMID： 3877861     DOI： 10.1007/bf02123696

Source DB:  PubMed          Journal:  Med Microbiol Immunol        ISSN： 0300-8584            Impact factor:   3.402


  10 in total

1.  Enzyme immunoassays in diagnostic medicine. Theory and practice.

Authors:  A Voller; D E Bidwell; A Bartlett
Journal:  Bull World Health Organ       Date:  1976       Impact factor: 9.408

2.  Preparation of iodine-131 labelled human growth hormone of high specific activity.

Authors:  W M HUNTER; F C GREENWOOD
Journal:  Nature       Date:  1962-05-05       Impact factor: 49.962

3.  Immunological response to outer membrane vesicles of Haemophilus influenzae in patients with acute sinusitis.

Authors:  T Harada; Y Sakakura; Y Miyoshi
Journal:  Rhinology       Date:  1986-03       Impact factor: 3.681

4.  Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes.

Authors:  E Engvall; P Perlmann
Journal:  J Immunol       Date:  1972-07       Impact factor: 5.422

5.  Experimental Haemophilus influenzae type b meningitis: immunological investigation of the infant rat model.

Authors:  D M Granoff; R Rockwell
Journal:  Infect Immun       Date:  1978-06       Impact factor: 3.441

6.  Quantitation of nasal secretory IgA by enzyme-linked immunosorbent assay.

Authors:  Y Hamaguchi; M Ohi; Y Sakakura; T Mukojima
Journal:  Int Arch Allergy Appl Immunol       Date:  1982

7.  Characterization of serologically dominant outer membrane proteins of Neisseria gonorrhoeae.

Authors:  R L McDade; K H Johnston
Journal:  J Bacteriol       Date:  1980-03       Impact factor: 3.490

8.  Polysaccharide vaccines of group A Neisseria meningtitidis and Haemophilus influenzae type b: a field trial in Finland.

Authors:  P H Mäkelä; H Peltola; H Käyhty; H Jousimies; O Pettay; E Ruoslahti; A Sivonen; O V Renkonen
Journal:  J Infect Dis       Date:  1977-08       Impact factor: 5.226

9.  A simplification of the protein assay method of Lowry et al. which is more generally applicable.

Authors:  G L Peterson
Journal:  Anal Biochem       Date:  1977-12       Impact factor: 3.365

10.  Quantitative determination of the human immune response to immunization with meningococcal vaccines.

Authors:  E C Gotschlich; M Rey; R Triau; K J Sparks
Journal:  J Clin Invest       Date:  1972-01       Impact factor: 14.808
  10 in total

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