Literature DB >> 6796959

Microinjection of a rabbit beta-globin gene into zygotes and its subsequent expression in adult mice and their offspring.

T E Wagner, P C Hoppe, J D Jollick, D R Scholl, R L Hodinka, J B Gault.   

Abstract

We have transferred a gene coding for rabbit beta-globin into the male pronucleus of mouse zygotes by direct microinjection. Some of these zygotes developed into mature mice which contained this gene and appeared to be producing a rabbit globin. Evidence for the presence of the gene in these animals was provided by Southern blot hybridization analysis. Evidence for the expression of the rabbit gene in these transformed mice and their offspring was provided by hemoglobin isoelectric focusing analysis and specific serological reactivity between mouse anti-rabbit hemoglobin antiserum and a hemolysate from the mice that developed from the microinjected zygotes. The use of this zygote transformation may allow the introduction and expression of a broad range of genetic elements in mammals.

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Year:  1981        PMID： 6796959      PMCID： PMC349042          DOI： 10.1073/pnas.78.10.6376

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  25 in total

1.  Transformation of mammalian cells with genes from procaryotes and eucaryotes.

Authors:  M Wigler; R Sweet; G K Sim; B Wold; A Pellicer; E Lacy; T Maniatis; S Silverstein; R Axel
Journal:  Cell       Date:  1979-04       Impact factor: 41.582

2.  Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donor.

Authors:  M Wigler; A Pellicer; S Silverstein; R Axel
Journal:  Cell       Date:  1978-07       Impact factor: 41.582

3.  Intraspecies transfer via total cellular DNA of the gene for hypoxanthine phosphoribosyltransferase into cultured mouse cells.

Authors:  K Willecke; M Klomfass; R Mierau; J Döhmer
Journal:  Mol Gen Genet       Date:  1979-02-26

4.  Nuclear protein changes in the maternally and paternally derived chromatin at fertilization.

Authors:  M Kunkle; F J Longo; B E Magun
Journal:  J Exp Zool       Date:  1978-03

Review 5.  When sperm meets egg: biochemical mechanisms of gamete interaction.

Authors:  B M Shapiro; E M Eddy
Journal:  Int Rev Cytol       Date:  1980

6.  Subunit structure of Eutherian sperm chromatin.

Authors:  T E Wagner; J E Sliwinski; D B Shewmaker
Journal:  Arch Androl       Date:  1978

7.  DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells.

Authors:  M Wigler; A Pellicer; S Silverstein; R Axel; G Urlaub; L Chasin
Journal:  Proc Natl Acad Sci U S A       Date:  1979-03       Impact factor: 11.205

8.  Resolution of products of the duplicated hemoglobin alpha-chain loci by isoelectric focusing.

Authors:  J B Whitney; G T Copland; L C Skow; E S Russell
Journal:  Proc Natl Acad Sci U S A       Date:  1979-02       Impact factor: 11.205

9.  Transformation of the gene for hypoxanthine phosphoribosyltransferase.

Authors:  L H Graf; G Urlaub; L A Chasin
Journal:  Somatic Cell Genet       Date:  1979-11

10.  Rabbit beta-globin mRNA production in mouse L cells transformed with cloned rabbit beta-globin chromosomal DNA.

Authors:  N Mantei; W Boll; C Weissmann
Journal:  Nature       Date:  1979-09-06       Impact factor: 49.962
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  66 in total

Review 1.  Molecular ophthalmology: an update on animal models for retinal degenerations and dystrophies.

Authors:  F Hafezi; C Grimm; B C Simmen; A Wenzel; C E Remé
Journal:  Br J Ophthalmol       Date:  2000-08       Impact factor: 4.638

2.  One hundred years of mouse genetics: an intellectual history. II. The molecular revolution (1981-2002).

Authors:  Kenneth Paigen
Journal:  Genetics       Date:  2003-04       Impact factor: 4.562

3.  Capricious Cre: the devil is in the details.

Authors:  Christopher D Morrison; Heike Münzberg
Journal:  Endocrinology       Date:  2012-03       Impact factor: 4.736

4.  Inhibition of Moloney murine leukemia virus-induced leukemia in transgenic mice expressing antisense RNA complementary to the retroviral packaging sequences.

Authors:  L Han; J S Yun; T E Wagner
Journal:  Proc Natl Acad Sci U S A       Date:  1991-05-15       Impact factor: 11.205

5.  Production of transgenic mice.

Authors:  J Richa
Journal:  Mol Biotechnol       Date:  2001-03       Impact factor: 2.695

6.  Expression of Growth Hormone Genes in Transgenic Mice.

Authors:  Richard D Palmiter; Robert E Hammer; Ralph L Brinster
Journal:  Banbury Rep       Date:  1985

7.  Targeted overexpression of luteinizing hormone in transgenic mice leads to infertility, polycystic ovaries, and ovarian tumors.

Authors:  K A Risma; C M Clay; T M Nett; T Wagner; J Yun; J H Nilson
Journal:  Proc Natl Acad Sci U S A       Date:  1995-02-28       Impact factor: 11.205

8.  Expression of T-cell receptor alpha-chain genes in transgenic mice.

Authors:  L J Berg; B Fazekas de St Groth; F Ivars; C C Goodnow; S Gilfillan; H J Garchon; M M Davis
Journal:  Mol Cell Biol       Date:  1988-12       Impact factor: 4.272

9.  Factors affecting the efficiency of introducing foreign DNA into mice by microinjecting eggs.

Authors:  R L Brinster; H Y Chen; M E Trumbauer; M K Yagle; R D Palmiter
Journal:  Proc Natl Acad Sci U S A       Date:  1985-07       Impact factor: 11.205

10.  Dramatic growth of mice that develop from eggs microinjected with metallothionein-growth hormone fusion genes.

Authors:  R D Palmiter; R L Brinster; R E Hammer; M E Trumbauer; M G Rosenfeld; N C Birnberg; R M Evans
Journal:  Nature       Date:  1982-12-16       Impact factor: 49.962
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