Literature DB >> 6796045

Thermal stabilization of glucose oxidase and glucoamylase by physical entrapment.

V Basaveswara Rao, N V Sastri, P V Subba Rao.   

Abstract

Physical entrapment was used as an approach to achieve thermal stabilization of enzymes. The t 1/2 values for the thermoinactivation of glucose oxidase and glucoamylase were increased several-fold by their entrapment in polyacrylamide gels. In polyacrylate gels the individual enzymes behaved differently, probably owing to microenvironmental effects arising by the polyelectrolyte nature of the carrier.

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Year:  1981        PMID： 6796045      PMCID： PMC1162617          DOI： 10.1042/bj1930389

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  10 in total

1.  A WATER-INSOLUBLE POLYANIONIC DERIVATIVE OF TRYPSIN. I. PREPARATION AND PROPERTIES.

Authors:  Y LEVIN; M PECHT; L GOLDSTEIN; E KATCHALSKI
Journal:  Biochemistry       Date:  1964-12       Impact factor: 3.162

2.  A WATER-INSOLUBLE POLYANIONIC DERIVATIVE OF TRYPSIN. II. EFFECT OF THE POLYELECTROLYTE CARRIER ON THE KINETIC BEHAVIOR OF THE BOUND TRYPSIN.

Authors:  L GOLDSTEIN; Y LEVIN; E KATCHALSKI
Journal:  Biochemistry       Date:  1964-12       Impact factor: 3.162

3.  The principles of enzyme stabilization. II. Increase in the thermostability of enzymes as a result of multipoint noncovalent interaction with a polymeric support.

Authors:  K Martinek; A M Klibanov; V S Goldmacher; A V Tchernysheva; V V Mozhaev; I V Berezin; B O Glotov
Journal:  Biochim Biophys Acta       Date:  1977-11-23

4.  The principles of enzyme stabilization. I. Increase in thermostability of enzymes covalently bound to a complementary surface of a polymer support in a multipoint fashion.

Authors:  K Martinek; A M Klibanov; V S Goldmacher; I V Berezin
Journal:  Biochim Biophys Acta       Date:  1977-11-23

Review 5.  Enzyme stabilization by immobilization.

Authors:  A M Klibanov
Journal:  Anal Biochem       Date:  1979-02       Impact factor: 3.365

6.  The denaturation by urea and guanidinium chloride of trypsin and N-acetylated-trypsin derivatives bound to Sephadex and agarose.

Authors:  D Gabel
Journal:  Eur J Biochem       Date:  1973-03-01

7.  Papain-collodion membranes. I. Preparation and properties.

Authors:  R Goldman; O Kedem; I H Silman; S R Caplan; E Katchalski
Journal:  Biochemistry       Date:  1968-02       Impact factor: 3.162

8.  Effect of local pH changes caused by substrate hydrolysis on the activity of membrane-bound acetylcholinesterase.

Authors:  H I Silman; A Karlin
Journal:  Proc Natl Acad Sci U S A       Date:  1967-10       Impact factor: 11.205

9.  Purification and characterization of a thermostable glucoamylase from the thermophilic fungus Thermomyces lanuginosus.

Authors:  V Basaveswara Rao; N V Sastri; P V Subba Rao
Journal:  Biochem J       Date:  1981-02-01       Impact factor: 3.857

Review 10.  Effect of the microenvironment on the mode of action of immobilized enzymes.

Authors:  E Katchalski; I Silman; R Goldman
Journal:  Adv Enzymol Relat Areas Mol Biol       Date:  1971
  10 in total
  2 in total

Review 1.  Thermophilic fungi: their physiology and enzymes.

Authors:  R Maheshwari; G Bharadwaj; M K Bhat
Journal:  Microbiol Mol Biol Rev       Date:  2000-09       Impact factor: 11.056

Review 2.  Enzyme stabilization: state of the art.

Authors:  L Gianfreda; M R Scarfi
Journal:  Mol Cell Biochem       Date:  1991-02-02       Impact factor: 3.396
  2 in total

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