Literature DB >> 6792282

A rapid solid-phase enzyme-linked binding assay for screening monoclonal antibodies to cell surface antigens.

S P Cobbold, H Waldmann.   

Abstract

A solid-phase enzyme-linked binding assay is described for screening monoclonal antibodies to cell surface antigens. E. coli beta-galactosidase was coupled to rabbit anti-rat Ig and used to detect the binding of rat monoclonal antibodies to cells which had been fixed to the wells of microtitre plates using a combination of poly-L-lysine and glutaraldehyde. This method was found to be advantageous for the large screening of monoclonal antibodies with a panel of cell types, and has been useful in the selection of antibodies which would be candidates for differentiation markers within the human and mouse haemopoietic systems.

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Year:  1981        PMID： 6792282     DOI： 10.1016/0022-1759(81)90340-9

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  20 in total

1.  A role for CD4+ but not CD8+ T cells in immunity to Schistosoma mansoni induced by 20 krad-irradiated and Ro 11-3128-terminated infections.

Authors:  D A Vignali; P Crocker; Q D Bickle; S Cobbold; H Waldmann; M G Taylor
Journal:  Immunology       Date:  1989-08       Impact factor: 7.397

2.  CCN4 regulates vascular smooth muscle cell migration and proliferation.

Authors:  Hao Liu; Wenpeng Dong; Zhiqi Lin; Jingbo Lu; Heng Wan; Zhongxin Zhou; Zhengjun Liu
Journal:  Mol Cells       Date:  2013-06-25       Impact factor: 5.034

3.  Development of new immunoradiometric assay for CA 125 antigen using two monoclonal antibodies produced by immunizing lung cancer cells.

Authors:  M Kunimatsu; K Endo; T Nakashima; T Awaji; T Saga; Y Watanabe; Y Kawamura; H Ohta; M Koizumi; H Sakahara
Journal:  Ann Nucl Med       Date:  1988-11       Impact factor: 2.668

4.  A simple and sensitive solid-phase radioimmunoassay for the assay of human TSH antibody. Use in screening hybridoma cell lines.

Authors:  S Iwasa; S Pincus; E Saito; N P Laborde; W D Odell
Journal:  Appl Biochem Biotechnol       Date:  1983-08       Impact factor: 2.926

5.  Enzyme-linked immunosorbent assay for the detection of serum antibody to outer membrane proteins of Treponema pallidum.

Authors:  J Morrison-Plummer; J F Alderete; J B Baseman
Journal:  Br J Vener Dis       Date:  1983-04

6.  A solid-phase radioimmunoassay to detect antibodies produced by hybridomas to antigens derived from human melanoma cells.

Authors:  P J Kelleher; H L Mathews; L K Woods; R S Farr; P Minden
Journal:  Cancer Immunol Immunother       Date:  1983       Impact factor: 6.968

7.  Production of monoclonal antibodies to Listeria monocytogenes and their application to determine the virulence of isolates from channel catfish.

Authors:  S Erdenlig; A J Ainsworth; F W Austin
Journal:  Appl Environ Microbiol       Date:  1999-07       Impact factor: 4.792

8.  Coxsackievirus B-3 myocarditis. Identification of different pathogenic mechanisms in DBA/2 and Balb/c mice.

Authors:  S A Huber; P A Lodge
Journal:  Am J Pathol       Date:  1986-02       Impact factor: 4.307

9.  Whole-bacterial cell enzyme-linked immunosorbent assay for Streptococcus sanguis fimbrial antigens.

Authors:  B L Elder; D K Boraker; P M Fives-Taylor
Journal:  J Clin Microbiol       Date:  1982-07       Impact factor: 5.948

10.  Enzyme linked immunosorbent assay for detecting antibody to Trichomonas vaginalis: use of whole cells and aqueous extract as antigen.

Authors:  J F Alderete
Journal:  Br J Vener Dis       Date:  1984-06
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