Literature DB >> 6792218

Improvements in the passive immune hemolysis test for assaying enterotoxigenic Escherichia coli.

A F de Castro, M B Serafim, J A Gomes, M S Gatti.   

Abstract

The sensitivity of the passive immune hemolysis test for the detection of heat-labile enterotoxin from Escherichia coli was increased when the test was carried out with Veronal-buffered saline plus Ca2+ and Mg2+ as diluent, instead of phosphate-buffered saline. The performance of the test was further improved by using stationary cultures to which mitomycin C had been added at the end of the lag phase.

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Year:  1980        PMID： 6792218      PMCID： PMC273679          DOI： 10.1128/jcm.12.5.714-717.1980

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  7 in total

1.  A quantitative complement fixation test: titration of luetic sera by the unit of 50 per cent hemolysis.

Authors:  G J STEIN; D VAN NGU
Journal:  J Immunol       Date:  1950-07       Impact factor: 5.422

2.  Factors influencing heat-labile Escherichia coli enterotoxin activity.

Authors:  D H Mundell; C R Anselmo; R M Wishnow
Journal:  Infect Immun       Date:  1976-08       Impact factor: 3.441

3.  Detection of heat-labile Escherichia coli enterotoxin with the use of adrenal cells in tissue culture.

Authors:  S T Donta; H W Moon; S C Whipp
Journal:  Science       Date:  1974-01-25       Impact factor: 47.728

4.  Assay of Escherichia coli heat-labile enterotoxin with vero cells.

Authors:  J I Speirs; S Stavric; J Konowalchuk
Journal:  Infect Immun       Date:  1977-05       Impact factor: 3.441

5.  Direct serological assay for the heat-labile enterotoxin of Escherichia coli, using passive immune hemolysis.

Authors:  D J Evans; D G Evans
Journal:  Infect Immun       Date:  1977-05       Impact factor: 3.441

6.  Polymyxin B-Induced Release of Low-Molecular-Weight, Heat-Labile Enterotoxin from Escherichia coli.

Authors:  D J Evans; D G Evans; S L Gorbach
Journal:  Infect Immun       Date:  1974-11       Impact factor: 3.441

7.  Cyclic adenosine monophosphate and alteration of Chinese hamster ovary cell morphology: a rapid, sensitive in vitro assay for the enterotoxins of Vibrio cholerae and Escherichia coli.

Authors:  R L Guerrant; L L Brunton; T C Schnaitman; L I Rebhun; A G Gilman
Journal:  Infect Immun       Date:  1974-08       Impact factor: 3.441
  7 in total
  6 in total

1.  Single radial immune hemolysis test for detection of Escherichia coli thermolabile enterotoxin.

Authors:  M B Serafim; A F de Castro; M B Leonardo; A R Monteiro
Journal:  J Clin Microbiol       Date:  1981-11       Impact factor: 5.948

2.  Stability of thermolabile (LT) enterotoxin produced from enterotoxigenic Escherichia coli strains maintained in vitro.

Authors:  M S Gatti; M B Serafim; A F Pestana de Castro; A R Monteiro
Journal:  Med Microbiol Immunol       Date:  1986       Impact factor: 3.402

3.  Evaluation of a defined medium for the production of both thermolabile (LT) and thermostable (ST) enterotoxins of Escherichia coli.

Authors:  C F Fontes; L C Ricci; M S Oliveira; M S Gatti; A F Pestana de Castro
Journal:  Med Microbiol Immunol       Date:  1982       Impact factor: 3.402

4.  Detection of heat-labile (LT) enterotoxin of enterotoxigenic Escherichia coli by the radial immune hemolysis test: a modification for clinical use.

Authors:  T Yano; M S Oliveira; C F Fontes; A C de Almeida; A F de Castro
Journal:  Med Microbiol Immunol       Date:  1982       Impact factor: 3.402

5.  Storage of Escherichia coli thermolabile enterotoxin on filter papers for assay by immune haemolysis reactions.

Authors:  L C Ricci; C F Fontes; P S Siqueira; A F Pestana de Castro
Journal:  Med Microbiol Immunol       Date:  1984       Impact factor: 3.402

6.  Indirect haemagglutination test for the detection of thermolabile (LT) enterotoxin from Escherichia coli.

Authors:  L C Ricci; A F de Castro
Journal:  Med Microbiol Immunol       Date:  1986       Impact factor: 3.402
  6 in total

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