Literature DB >> 6790718

Rapid cataloging of ribonuclease T1 resistant oligonucleotides from ribosomal RNAs for phylogenetic studies.

E Stackebrandt, W Ludwig, K H Schleifer, H J Gross.   

Abstract

A rapid and simple method of oligonucleotide cataloging for phylogenetic studies is presented. It involves in vitro 5'-32P-labelling of RNase T1 - resistant oligonucleotides of ribosomal 16S RNA and finger-printing by high voltage electrophoresis and gradient thinlayer chromatography. Oligonucleotide sequences are established by the mobility shift method, using controlled alkali cleavage, high voltage electrophoresis and homochromatography. These procedures facilitate in particular the analysis of long RNase T1 - resistant oligonucleotides. Oligonucleotide catalogs are established fo three Actinomycetes, namely Oerskovia turbata, Actinoplanes philippinensis and Ampullariella regularis. These catalogs are equivalent to those obtained by methods which were described by Sanger and Woese.

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Year:  1981        PMID： 6790718     DOI： 10.1007/BF01732760

Source DB:  PubMed          Journal:  J Mol Evol        ISSN： 0022-2844            Impact factor:   2.395


  28 in total

1.  3'-Phosphatase activity in T4 polynucleotide kinase.

Authors:  V Cameron; O C Uhlenbeck
Journal:  Biochemistry       Date:  1977-11-15       Impact factor: 3.162

2.  The use of nuclease P1 in sequence analysis of end group labeled RNA.

Authors:  M Silberklang; A M Gillum; U L RajBhandary
Journal:  Nucleic Acids Res       Date:  1977-12       Impact factor: 16.971

3.  Phylogenetic structure of the prokaryotic domain: the primary kingdoms.

Authors:  C R Woese; G E Fox
Journal:  Proc Natl Acad Sci U S A       Date:  1977-11       Impact factor: 11.205

4.  Absence of the sequence G-T-psi-C-G(A)- in several eukaryotic cytoplasmic initiator transfer RNAs.

Authors:  M Simsek; J Ziegenmeyer; J Heckman; U L Rajbhandary
Journal:  Proc Natl Acad Sci U S A       Date:  1973-04       Impact factor: 11.205

5.  Physical characterization and simultaneous purification of bacteriophage T4 induced polynucleotide kinase, polynucleotide ligase, and deoxyribonucleic acid polymerase.

Authors:  A Panet; J H van de Sande; P C Loewen; H G Khorana; A J Raae; J R Lillehaug; K Kleppe
Journal:  Biochemistry       Date:  1973-12-04       Impact factor: 3.162

6.  An improved method for transferring nucleotides from electrophoresis strips to thin layers of ion-exchange cellulose.

Authors:  E M Southern
Journal:  Anal Biochem       Date:  1974-11       Impact factor: 3.365

7.  Purification and properties of the replicative intermediate of the RNA bacteriophage R17.

Authors:  R M Franklin
Journal:  Proc Natl Acad Sci U S A       Date:  1966-06       Impact factor: 11.205

8.  A comparison of the 16S ribosomal RNAs from mesophilic and thermophilic bacilli: some modifications in the Sanger method for RNA sequencing.

Authors:  C Woese; M Sogin; D Stahl; B J Lewis; L Bonen
Journal:  J Mol Evol       Date:  1976-04-09       Impact factor: 2.395

9.  Nucleotide sequence of three isoaccepting lysine tRNAs from rabbit liver and SV40-transformed mouse fibroblasts.

Authors:  M Raba; K Limburg; M Burghagen; J R Katze; M Simsek; J E Heckman; U L Rajbhandary; H J Gross
Journal:  Eur J Biochem       Date:  1979-06

10.  Are extreme halophiles actually "bacteria"?

Authors:  L J Magrum; K R Luehrsen; C R Woese
Journal:  J Mol Evol       Date:  1978-05-12       Impact factor: 2.395
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  7 in total

1.  Circularization of linear viroid RNA via 2'-phosphomonoester, 3', 5'-phosphodiester bonds by a novel type of RNA ligase from wheat germ and Chlamydomonas.

Authors:  Y Kikuchi; K Tyc; W Filipowicz; H L Sänger; H J Gross
Journal:  Nucleic Acids Res       Date:  1982-12-11       Impact factor: 16.971

Review 2.  A historical perspective on mycorrhizal mutualism emphasizing arbuscular mycorrhizas and their emerging challenges.

Authors:  Antoine Sportes; Mathilde Hériché; Raphaël Boussageon; Pierre-Antoine Noceto; Diederik van Tuinen; Daniel Wipf; Pierre Emmanuel Courty
Journal:  Mycorrhiza       Date:  2021-10-16       Impact factor: 3.856

3.  A phylogenetic analysis of Legionella.

Authors:  W Ludwig; E Stackebrandt
Journal:  Arch Microbiol       Date:  1983-08       Impact factor: 2.552

4.  Evidence for unlinked rrn operons in the Planctomycete Pirellula marina.

Authors:  W Liesack; E Stackebrandt
Journal:  J Bacteriol       Date:  1989-09       Impact factor: 3.490

5.  A phylogenetic survey of budding, and/or prosthecate, non-phototrophic eubacteria: membership of Hyphomicrobium, Hyphomonas, Pedomicrobium, Filomicrobium, Caulobacter and "dichotomicrobium" to the alpha-subdivision of purple non-sulfur bacteria.

Authors:  E Stackebrandt; A Fischer; T Roggentin; U Wehmeyer; D Bomar; J Smida
Journal:  Arch Microbiol       Date:  1988       Impact factor: 2.552

6.  Ligation of endogenous tRNA 3' half molecules to their corresponding 5' halves via 2'-phosphomonoester,3',5'-phosphodiester bonds in extracts of Chlamydomonas.

Authors:  K Tyc; Y Kikuchi; M Konarska; W Filipowicz; H J Gross
Journal:  EMBO J       Date:  1983       Impact factor: 11.598

7.  The Mycobacterium phlei Genome: Expectations and Surprises.

Authors:  Sarbashis Das; B M Fredrik Pettersson; Phani Rama Krishna Behra; Malavika Ramesh; Santanu Dasgupta; Alok Bhattacharya; Leif A Kirsebom
Journal:  Genome Biol Evol       Date:  2016-04-08       Impact factor: 3.416
  7 in total

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