Literature DB >> 6788835

Immunofluorescent localization of cathepsins B and D in human fibroblasts.

J S Mort, A R Poole, R S Decker.   

Abstract

The proteinase cathepsin B has been localized in human fibroblasts using a monospecific antiserum that reacts in a precipitating manner with the denatured enzyme only. The distribution of staining as revealed by both direct and indirect immunofluorescence indicates a typical lysosomal localization similar to that observed for cathepsin D. Using dual localization methods employing fluorescein- and tetramethylrhodamine-labeled affinity purified antibodies both proteinases were usually observed in the same lysosome, indicating a combined role in intracellular digestion.

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Year:  1981        PMID： 6788835     DOI： 10.1177/29.5.6788835

Source DB:  PubMed          Journal:  J Histochem Cytochem        ISSN： 0022-1554            Impact factor:   2.479


  11 in total

1.  Proteolytic processing and glycosylation of cathepsin B. The role of the primary structure of the latent precursor and of the carbohydrate moiety for cell-type-specific molecular forms of the enzyme.

Authors:  L Mach; K Stüwe; A Hagen; C Ballaun; J Glössl
Journal:  Biochem J       Date:  1992-03-01       Impact factor: 3.857

2.  Link protein as a monitor in situ of endogenous proteolysis in adult human articular cartilage.

Authors:  Q Nguyen; J Liu; P J Roughley; J S Mort
Journal:  Biochem J       Date:  1991-08-15       Impact factor: 3.857

3.  Isolation and characterization of cathepsin B from bovine brain.

Authors:  J D Bradley; J N Whitaker
Journal:  Neurochem Res       Date:  1986-06       Impact factor: 3.996

4.  Localization of cathepsin B activity in fibroblasts and chondrocytes by continuous monitoring of the formation of a final fluorescent reaction product using 5-nitrosalicylaldehyde.

Authors:  C J Van Noorden; I M Vogels; V Everts; W Beertsen
Journal:  Histochem J       Date:  1987-09

5.  Immunoelectron microscopic localization of cathepsin D in lysosomes of rat nerve cells.

Authors:  S Yokota; S Atsumi
Journal:  Histochemistry       Date:  1983

6.  Localization of cathepsin D in rat liver. Immunocytochemical study using post-embedding immunoenzyme and protein A-gold techniques.

Authors:  S Yokota; H Tsuji; K Kato
Journal:  Histochemistry       Date:  1985

7.  A cysteine proteinase secreted from human breast tumours is immunologically related to cathepsin B.

Authors:  A D Recklies; A R Poole; J S Mort
Journal:  Biochem J       Date:  1982-12-01       Impact factor: 3.857

8.  Immunological demonstration of intestinal absorption and digestion of protein macromolecules in the trout (Salmo gairdneri).

Authors:  U Georgopoulou; M F Sire; J M Vernier
Journal:  Cell Tissue Res       Date:  1986       Impact factor: 5.249

9.  Are Z-Arg-Gly-Phe-Phe-Leu-MNA and Z-Arg-Gly-Phe-Phe-Pro-MNA suitable substrates for the demonstration of cathepsin D activity?

Authors:  Z Lojda; J Smídová; A Barth; H Ueberberg
Journal:  Histochemistry       Date:  1988

10.  Correct processing of the kiwifruit protease actinidin in transgenic tobacco requires the presence of the C-terminal propeptide.

Authors:  W Paul; J Amiss; R Try; U Praekelt; R Scott; H Smith
Journal:  Plant Physiol       Date:  1995-05       Impact factor: 8.340
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