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Literature DB >> 6788830

Improved methods for the fluorographic detection of weak beta-emitting radioisotopes in Agarose and acrylamide gel electrophoresis media.

Abstract

The use of acetic acid as a solvent for diphenyloxazole (PPO) in fluorographic procedures has been investigated. It is demonstrated to be superior to both dimethyl sulfoxide and methanol with respect to its suitability in both agarose and acrylamide gel electrophoresis systems. In addition, a method has been developed for impregnating fragile gels such as those used for immunodiffusion with PPO in preparation for fluorography.

Entities: Chemical Gene

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Year: 1981 PMID： 6788830 DOI： 10.1016/0165-022x(81)90074-9

Source DB: PubMed Journal: J Biochem Biophys Methods ISSN： 0165-022X

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Cited

17 in total

1. Polymorphisms within the HLA-DRw6 haplotype. II. Protein charge heterogeneity reflects MLC subtyping of HLA-DRw6 homozygous cells.

Authors: M L Bosch; A Termijtelen; R Gerrets; G M Schreuder; R E Bontrop; M J Giphart
Journal: Immunogenetics Date: 1985 Impact factor: 2.846

10. Coordinated regulation of type I and type III collagen production and mRNA levels of pro alpha 1(I) and pro alpha 2(I) collagen in cultured morphea fibroblasts.

Authors: T Vuorio; J K Mäkelä; V M Kähäri; E Vuorio
Journal: Arch Dermatol Res Date: 1987 Impact factor: 3.017

Improved methods for the fluorographic detection of weak beta-emitting radioisotopes in Agarose and acrylamide gel electrophoresis media.

1. Polymorphisms within the HLA-DRw6 haplotype. II. Protein charge heterogeneity reflects MLC subtyping of HLA-DRw6 homozygous cells.

2. Localisation and cellular origin of hyaluronectin.

3. Topoisomerase inhibitors can selectively interfere with different stages of simian virus 40 DNA replication.

4. In vivo labeling of myelin lipids and proteolipid protein with [3H]myristate, [14C]linoleate, and [14C]linolenate.

5. Development of a bifunctional crosslinking agent with potential for the preparation of immunotoxins.

6. Swiveling and decatenation of replicating simian virus 40 genomes in vivo.

7. Distribution and degradation of biotin-containing carboxylases in human cell lines.

8. Association and release of the major intrinsic membrane glycoprotein from peripheral nerve myelin.

9. Human recombinant interleukin-1 regulates cellular mRNA levels of dermatan sulphate proteoglycan core protein.

10. Coordinated regulation of type I and type III collagen production and mRNA levels of pro alpha 1(I) and pro alpha 2(I) collagen in cultured morphea fibroblasts.