Literature DB >> 3267139

Development of a bifunctional crosslinking agent with potential for the preparation of immunotoxins.

J A McKenzie1, R L Raison, D E Rivett.   

Abstract

A new protein crosslinking agent, 2,3-dibromopropionyl-N-hydroxysuccinimide ester, has been synthesized and characterized. The potential use of this compound as a temperature-controllable heterobifunctional crosslinking agent has been investigated using model systems and its reactivity compared with that of chlorambucil-N-hydroxysuccinimide ester. The coupling of 14C-labeled phenylethylamine to lysozyme has been used to illustrate the feasibility of the use of this crosslinking agent for the synthesis of immunotoxins.

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Year:  1988        PMID： 3267139     DOI： 10.1007/bf01024876

Source DB:  PubMed          Journal:  J Protein Chem        ISSN： 0277-8033


  9 in total

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Authors:  R E CANFIELD
Journal:  J Biol Chem       Date:  1963-08       Impact factor: 5.157

2.  The covalent binding of daunomycin and adriamycin to antibodies, with retention of both drug and antibody activities.

Authors:  E Hurwitz; R Levy; R Maron; M Wilchek; R Arnon; M Sela
Journal:  Cancer Res       Date:  1975-05       Impact factor: 12.701

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Authors:  J King; U K Laemmli
Journal:  J Mol Biol       Date:  1971-12-28       Impact factor: 5.469

4.  Preparation of antibody-toxin conjugates.

Authors:  A J Cumber; J A Forrester; B M Foxwell; W C Ross; P E Thorpe
Journal:  Methods Enzymol       Date:  1985       Impact factor: 1.600

5.  Methods for the preparation of enzyme-antibody conjugates for use in enzyme immunoassay.

Authors:  M J O'Sullivan; V Marks
Journal:  Methods Enzymol       Date:  1981       Impact factor: 1.600

Review 6.  The preparation and cytotoxic properties of antibody-toxin conjugates.

Authors:  P E Thorpe; W C Ross
Journal:  Immunol Rev       Date:  1982       Impact factor: 12.988

7.  Improved methods for the fluorographic detection of weak beta-emitting radioisotopes in Agarose and acrylamide gel electrophoresis media.

Authors:  D E Pulleyblank; G M Booth
Journal:  J Biochem Biophys Methods       Date:  1981-06

8.  Specific targeting of chlorambucil to tumors with the use of monoclonal antibodies.

Authors:  M J Smyth; G A Pietersz; B J Classon; I F McKenzie
Journal:  J Natl Cancer Inst       Date:  1986-03       Impact factor: 13.506

9.  Protein thiolation and reversible protein-protein conjugation. N-Succinimidyl 3-(2-pyridyldithio)propionate, a new heterobifunctional reagent.

Authors:  J Carlsson; H Drevin; R Axén
Journal:  Biochem J       Date:  1978-09-01       Impact factor: 3.857
  9 in total
  1 in total

1.  Bifunctional cross-linking approaches for mass spectrometry-based investigation of nucleic acids and protein-nucleic acid assemblies.

Authors:  M Scalabrin; S M Dixit; M M Makshood; C E Krzemien; Daniele Fabris
Journal:  Methods       Date:  2018-05-10       Impact factor: 3.608
  1 in total

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