Literature DB >> 6787599

Distribution of protein I in mammalian brain as determined by a detergent-based radioimmunoassay.

S E Goelz, E J Nestler, B Chehrazi, P Greengard.   

Abstract

A radioimmunoassay has been developed for measuring protein I, a basic, neuron-specific protein associated with nerve terminals. The procedure utilizes the detergents NaDodSO4 and Nonidet P-40 to prevent nonspecific adsorption of this highly charged protein to various surfaces. By use of this procedure, it has been possible to show that protein I comprises approximately 0.4% of the total protein in cerebral cortex of several mammalian species. In addition, the amount of protein I was determined in about 40 regions of cat brain. The results suggest that measurement of protein I may provide a quantitative method for estimating the density of nerve terminals in various regions of the mammalian nervous system.

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Year:  1981        PMID： 6787599      PMCID： PMC319297          DOI： 10.1073/pnas.78.4.2130

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  15 in total

1.  Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A.

Authors:  S W Kessler
Journal:  J Immunol       Date:  1975-12       Impact factor: 5.422

2.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

Review 3.  Radioimmunoassay: a probe for the fine structure of biologic systems.

Authors:  R S Yalow
Journal:  Science       Date:  1978-06-16       Impact factor: 47.728

4.  A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity.

Authors:  I M Glynn; J B Chappell
Journal:  Biochem J       Date:  1964-01       Impact factor: 3.857

5.  Depolarizing agents and cyclic nucleotides regulate the phosphorylation of specific neuronal proteins in rat cerebral cortex slices.

Authors:  J Forn; P Greengard
Journal:  Proc Natl Acad Sci U S A       Date:  1978-10       Impact factor: 11.205

6.  Ca2+ and cyclic AMP regulate phosphorylation of same two membrane-associated proteins specific to nerve tissue.

Authors:  W Sieghart; J Forn; P Greengard
Journal:  Proc Natl Acad Sci U S A       Date:  1979-05       Impact factor: 11.205

7.  Multiple phosphorylation sites in protein I and their differential regulation by cyclic AMP and calcium.

Authors:  W B Huttner; P Greengard
Journal:  Proc Natl Acad Sci U S A       Date:  1979-10       Impact factor: 11.205

8.  Adenosine 3':5'-monophosphate-regulated phosphoprotein system of neuronal membranes. I. Solubilization, purification, and some properties of an endogenous phosphoprotein.

Authors:  T Ueda; P Greengard
Journal:  J Biol Chem       Date:  1977-07-25       Impact factor: 5.157

9.  Widespread distribution of protein I in the central and peripheral nervous systems.

Authors:  P De Camilli; T Ueda; F E Bloom; E Battenberg; P Greengard
Journal:  Proc Natl Acad Sci U S A       Date:  1979-11       Impact factor: 11.205

10.  Subcellular distribution in cerebral cortex of two proteins phosphorylated by a cAMP-dependent protein kinase.

Authors:  T Ueda; P Greengard; K Berzins; R S Cohen; F Blomberg; D J Grab; P Siekevitz
Journal:  J Cell Biol       Date:  1979-11       Impact factor: 10.539
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  22 in total

Review 1.  The formation of synapses in the central nervous system.

Authors:  Adriana Ferreira; Sabrina Paganoni
Journal:  Mol Neurobiol       Date:  2002-08       Impact factor: 5.590

2.  Evidence for widespread effects of noradrenaline on axon terminals in the rat frontal cortex.

Authors:  P Mobley; P Greengard
Journal:  Proc Natl Acad Sci U S A       Date:  1985-02       Impact factor: 11.205

3.  Synapsin III: developmental expression, subcellular localization, and role in axon formation.

Authors:  A Ferreira; H T Kao; J Feng; M Rapoport; P Greengard
Journal:  J Neurosci       Date:  2000-05-15       Impact factor: 6.167

4.  Widespread occurrence of "87 kDa," a major specific substrate for protein kinase C.

Authors:  K A Albert; S I Walaas; J K Wang; P Greengard
Journal:  Proc Natl Acad Sci U S A       Date:  1986-05       Impact factor: 11.205

Review 5.  Neuronal phosphoproteins. Mediators of signal transduction.

Authors:  P Greengard
Journal:  Mol Neurobiol       Date:  1987 Spring-Summer       Impact factor: 5.590

6.  A major calmodulin-binding protein common to various vertebrate tissues.

Authors:  H C Palfrey; W Schiebler; P Greengard
Journal:  Proc Natl Acad Sci U S A       Date:  1982-06       Impact factor: 11.205

7.  A quantitative dot-immunobinding assay for proteins using nitrocellulose membrane filters.

Authors:  R Jahn; W Schiebler; P Greengard
Journal:  Proc Natl Acad Sci U S A       Date:  1984-03       Impact factor: 11.205

8.  Translocation of synapsin I in response to depolarization of isolated nerve terminals.

Authors:  T S Sihra; J K Wang; F S Gorelick; P Greengard
Journal:  Proc Natl Acad Sci U S A       Date:  1989-10       Impact factor: 11.205

9.  Synapsin I (protein I) in different brain regions in senile dementia of Alzheimer type and in multi-infarct dementia.

Authors:  E Perdahl; R Adolfsson; I Alafuzoff; K A Albert; E J Nestler; P Greengard; B Winblad
Journal:  J Neural Transm       Date:  1984       Impact factor: 3.575

10.  Expression of autophosphorylating protein kinase 500 in normal and neoplastic rat cells.

Authors:  G Majumdar; M Cashel; R K Sharma
Journal:  Proc Natl Acad Sci U S A       Date:  1985-08       Impact factor: 11.205
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