Literature DB >> 6785758

Resonance Raman spectroscopy of specifically [epsilon-15N]lysine-labeled bacteriorhodopsin.

P V Argade, K J Rothschild, A H Kawamoto, J Herzfeld, W C Herlihy.   

Abstract

The possible interaction of a second lysine with the retinylidene Schiff base of bacteriorhodopsin (Lewis, A., Marcus, M. A., Ehrenberg, B. & Crespi, H. (1978) Proc. Natl. Acad. Sci. USA 75, 4642-4646) has been investigated by specific incorporation of 15N into the epsilon-amino groups of the lysine residues. Comparison of resonance Raman spectra of bacteriorhodopsin grown on 100%, 0%, and 50% labeled lysine demonstrates that 15N isotope effects on the Schiff base vibration can be accounted for by 15N labeling only at the Schiff base nitrogen. Our data also provide in situ confirmation of the linkage of the retinal chromophore with the epsilon-amino nitrogen of lysine.

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Year:  1981        PMID： 6785758      PMCID： PMC319188          DOI： 10.1073/pnas.78.3.1643

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  12 in total

1.  Experimental evidence for secondary protein-chromophore interactions at the Schiff base linkage in bacteriorhodopsin: Molecular mechanism for proton pumping.

Authors:  A Lewis; M A Marcus; B Ehrenberg; H Crespi
Journal:  Proc Natl Acad Sci U S A       Date:  1978-10       Impact factor: 11.205

Review 2.  Bacteriorhodopsin and the purple membrane of halobacteria.

Authors:  W Stoeckenius; R H Lozier; R A Bogomolni
Journal:  Biochim Biophys Acta       Date:  1979-03-14

3.  Amino acid analysis by gas-liquid chromatography of N-heptafluorobutyryl isobutyl esters. Complete resolution using a support-coated open-tubular capillary column.

Authors:  R J Pearce
Journal:  J Chromatogr       Date:  1977-06-01

4.  Tunable laser resonance raman spectroscopy of bacteriorhodopsin.

Authors:  A Lewis; J Spoonhower; R A Bogomolni; R H Lozier; W Stoeckenius
Journal:  Proc Natl Acad Sci U S A       Date:  1974-11       Impact factor: 11.205

5.  Growth and nutrition of extremely halophilic bacteria.

Authors:  M B Gochnauer; D J Kushner
Journal:  Can J Microbiol       Date:  1969-10       Impact factor: 2.419

6.  Partial primary structure of bacteriorhodopsin: sequencing methods for membrane proteins.

Authors:  G E Gerber; R J Anderegg; W C Herlihy; C P Gray; K Biemann; H G Khorana
Journal:  Proc Natl Acad Sci U S A       Date:  1979-01       Impact factor: 11.205

7.  Photochemical cycle of bacteriorhodopsin studied by resonance Raman spectroscopy.

Authors:  M Stockburger; W Klusmann; H Gattermann; G Massig; R Peters
Journal:  Biochemistry       Date:  1979-10-30       Impact factor: 3.162

8.  Photoreceptor protein from the purple membrane of Halobacterium halobium. Molecular weight and retinal binding site.

Authors:  J Bridgen; I D Walker
Journal:  Biochemistry       Date:  1976-02-24       Impact factor: 3.162

9.  Resonance Raman spectroscopy of the retinylidene chromophore in bacteriorhodopsin (bR570), bR560, M421, and other intermediates: structural conclusions based on kinetics, analogues, models, and isotopically labeled membranes.

Authors:  M A Marcus; A Lewis
Journal:  Biochemistry       Date:  1978-10-31       Impact factor: 3.162

10.  Resonance Raman studies of the primary photochemical event in visual pigments.

Authors:  B Aton; A G Doukas; D Narva; R H Callender; U Dinur; B Honig
Journal:  Biophys J       Date:  1980-01       Impact factor: 4.033
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  10 in total

1.  Resolution and polarization distribution in cryogenic DNP/MAS experiments.

Authors:  Alexander B Barnes; Björn Corzilius; Melody L Mak-Jurkauskas; Loren B Andreas; Vikram S Bajaj; Yoh Matsuki; Marina L Belenky; Johan Lugtenburg; Jagadishwar R Sirigiri; Richard J Temkin; Judith Herzfeld; Robert G Griffin
Journal:  Phys Chem Chem Phys       Date:  2010-05-08       Impact factor: 3.676

2.  Magnetic birefringence studies of dilute purple membrane suspensions.

Authors:  B A Lewis; C Rosenblatt; R G Griffin; J Courtemanche; J Herzfeld
Journal:  Biophys J       Date:  1985-02       Impact factor: 4.033

3.  Infrared evidence that the Schiff base of bacteriorhodopsin is protonated: bR570 and K intermediates.

Authors:  K J Rothschild; H Marrero
Journal:  Proc Natl Acad Sci U S A       Date:  1982-07       Impact factor: 11.205

4.  Evidence for a tyrosine protonation change during the primary phototransition of bacteriorhodopsin at low temperature.

Authors:  K J Rothschild; P Roepe; P L Ahl; T N Earnest; R A Bogomolni; S K Das Gupta; C M Mulliken; J Herzfeld
Journal:  Proc Natl Acad Sci U S A       Date:  1986-01       Impact factor: 11.205

5.  Polarized Fourier transform infrared spectroscopy of bacteriorhodopsin. Transmembrane alpha helices are resistant to hydrogen/deuterium exchange.

Authors:  T N Earnest; J Herzfeld; K J Rothschild
Journal:  Biophys J       Date:  1990-12       Impact factor: 4.033

6.  Participation of bacteriorhodopsin active-site lysine backbone in vibrations associated with retinal photochemistry.

Authors:  Y Gat; M Grossjean; I Pinevsky; H Takei; Z Rothman; H Sigrist; A Lewis; M Sheves
Journal:  Proc Natl Acad Sci U S A       Date:  1992-03-15       Impact factor: 11.205

7.  Low temperature FTIR study of the Schiff base reprotonation during the M-to-bR backphotoreaction: Asp 85 reprotonates two distinct types of Schiff base species at different temperatures.

Authors:  H Takei; Y Gat; M Sheves; A Lewis
Journal:  Biophys J       Date:  1992-12       Impact factor: 4.033

8.  Primary Transfer Step in the Light-Driven Ion Pump Bacteriorhodopsin: An Irreversible U-Turn Revealed by Dynamic Nuclear Polarization-Enhanced Magic Angle Spinning NMR.

Authors:  Qing Zhe Ni; Thach V Can; Eugenio Daviso; Marina Belenky; Robert G Griffin; Judith Herzfeld
Journal:  J Am Chem Soc       Date:  2018-03-12       Impact factor: 15.419

9.  Energy transformations early in the bacteriorhodopsin photocycle revealed by DNP-enhanced solid-state NMR.

Authors:  Melody L Mak-Jurkauskas; Vikram S Bajaj; Melissa K Hornstein; Marina Belenky; Robert G Griffin; Judith Herzfeld
Journal:  Proc Natl Acad Sci U S A       Date:  2008-01-14       Impact factor: 11.205

10.  Raman spectroscopy reveals direct chromophore interactions in the Leu/Gln105 spectral tuning switch of proteorhodopsins.

Authors:  Joel M Kralj; Elena N Spudich; John L Spudich; Kenneth J Rothschild
Journal:  J Phys Chem B       Date:  2008-08-22       Impact factor: 2.991
  10 in total

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