Literature DB >> 6780536

Purification of polypeptide P6 with a molecular weight of 6,000 from the vegetative chromosomes of Bacillus subtilis.

T Nakayama, Y Kurogi, M Irikura.   

Abstract

Folded chromosomes were prepared as membrane-associated complexes from vegetative cells of Bacillus subtilis by stepwise sucrose gradient centrifugation. From nucleoids, a deoxyribonucleic acid-bound polypeptide with a molecular weight of 6,000 (P6) was purified by KCl-(NH4)2SO4 salting out, diethylaminoethyl cellulose column chromatography, and deoxyribonucleic acid cellulose column chromatography. The amino acid composition of polypeptide P6 was determined.

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Year:  1981        PMID： 6780536      PMCID： PMC217203          DOI： 10.1128/jb.145.2.953-957.1981

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  14 in total

1.  The precision of ultraviolet absorption measurements in the Schmidt-Thannhauser procedure for nucleic acid estimation.

Authors:  A FLECK; H N MUNRO
Journal:  Biochim Biophys Acta       Date:  1962-05-14

2.  A microchemical determination of desoxyribonucleic acid.

Authors:  G CERIOTTI
Journal:  J Biol Chem       Date:  1952-09       Impact factor: 5.157

3.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

4.  Spore coat protein synthesis in cell-free systems from sporulating cells of Bacillus subtilis.

Authors:  T Nakayama; L E Munoz; Y Sadaie; R H Doi
Journal:  J Bacteriol       Date:  1978-09       Impact factor: 3.490

Review 5.  Unique biochemical events in bacterial sporulation.

Authors:  R S Hanson; J A Peterson; A A Yousten
Journal:  Annu Rev Microbiol       Date:  1970       Impact factor: 15.500

6.  Two proteins from cowpea mosaic virus.

Authors:  G J Wu; G Bruening
Journal:  Virology       Date:  1971-12       Impact factor: 3.616

7.  Identification of flagellin associated with the Bacillus subtilis folded chromosome.

Authors:  A M Guérout-Fleury; F Le Hégarat; L Hirschbein
Journal:  J Bacteriol       Date:  1980-03       Impact factor: 3.490

8.  The stability of messenger ribonucleic acid during sporulation in Bacillus subtilis.

Authors:  T J Leighton; R H Doi
Journal:  J Biol Chem       Date:  1971-05-25       Impact factor: 5.157

9.  Proteinase inhibitors from a mimosoideae legume, Albizzia julibrissin. Homologues of soybean trypsin inhibitor (Kunitz).

Authors:  S Odani; S Odani; T Ono; T Ikenaka
Journal:  J Biochem       Date:  1979-12       Impact factor: 3.387

10.  Folded chromosomes of vegetative Bacillus subtilis: composition and properties.

Authors:  N Guillen; F Le Hegaret; A M Fleury; L Hirschbein
Journal:  Nucleic Acids Res       Date:  1978-02       Impact factor: 16.971
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