Literature DB >> 6779813

The separation and purification of rat liver UDP-glucuronyltransferase activities towards testosterone and oestrone.

P J Weatherill, B Burchell.   

Abstract

1. Reconstitution of UDP-glucuronyltransferase preparations with phosphataidylcholine liposomes facilitated the purification of testosterone UDP-glucuronyltransferase. 2. Transferase activity towards testosterone co-purifies with that towards 4-nitrophenol. 3. UDP-glucuronyltransferase activity towards oestrone was separated from that towards testosterone. 4. These results suggest that testosterone and 4-nitrophenol may be glucuronidated by a different form of UDP-glucuronyltransferase from the one glucuronidating oestrone.

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Year:  1980        PMID： 6779813      PMCID： PMC1162010          DOI： 10.1042/bj1890377

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  17 in total

1.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

2.  Studies on the activation in vitro of glucuronyltransferase.

Authors:  A Winsnes
Journal:  Biochim Biophys Acta       Date:  1969-11-04

3.  Partial purification and kinetic properties of a soluble estrogen glucuronyltransferase from pig intestine.

Authors:  G S Rao; H Breuer
Journal:  J Biol Chem       Date:  1969-10-25       Impact factor: 5.157

4.  The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

Authors:  K Weber; M Osborn
Journal:  J Biol Chem       Date:  1969-08-25       Impact factor: 5.157

5.  An improved assay for steroid glucuronyltransferase in rat liver microsomes.

Authors:  G S Rao; G Haueter; M L Rao; H Breuer
Journal:  Anal Biochem       Date:  1976-07       Impact factor: 3.365

6.  Purification of UDP-glucuronyltransferase from untreated rat liver.

Authors:  B Burchell
Journal:  FEBS Lett       Date:  1977       Impact factor: 4.124

7.  Delipidation and reactivation of UDPglucuronosyltransferase from rat liver.

Authors:  P L Jansen; I M Arias
Journal:  Biochim Biophys Acta       Date:  1975-05-23

8.  Purification and properties of microsomal UDP-glucuronosyltransferase from rat liver.

Authors:  J P Gorski; C B Kasper
Journal:  J Biol Chem       Date:  1977-02-25       Impact factor: 5.157

9.  Studies on a testosterone glucuronyltransferase from the cytosol fraction of human liver.

Authors:  G S Rao; M L Rao; H Breuer
Journal:  Biochem J       Date:  1970-10       Impact factor: 3.857

10.  Partial purification and kinetics of oestriol 16 alpha-glucuronyltransferase from the cytosol fraction of human liver.

Authors:  G S Rao; M L Rao; H Breuer
Journal:  Biochem J       Date:  1970-07       Impact factor: 3.857
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  5 in total

1.  Isolation and characterization of multiple forms of rat liver UDP-glucuronate glucuronosyltransferase.

Authors:  J Roy Chowdhury; N Roy Chowdhury; C N Falany; T R Tephly; I M Arias
Journal:  Biochem J       Date:  1986-02-01       Impact factor: 3.857

2.  Genetic deficiency of androsterone UDP-glucuronosyltransferase activity in Wistar rats is due to the loss of enzyme protein.

Authors:  M Matsui; F Nagai
Journal:  Biochem J       Date:  1986-02-15       Impact factor: 3.857

3.  Development of multiple activities of UDP-glucuronyltransferase in human liver.

Authors:  J E Leakey; R Hume; B Burchell
Journal:  Biochem J       Date:  1987-05-01       Impact factor: 3.857

4.  Reconstitution of purified Wistar rat liver bilirubin UDP-glucuronyltransferase into Gunn-rat liver microsomes.

Authors:  B Burchell
Journal:  Biochem J       Date:  1982-03-01       Impact factor: 3.857

5.  Isolation of multiple normal and functionally defective forms of uridine diphosphate-glucuronosyltransferase from inbred Gunn rats.

Authors:  N Roy Chowdhury; F Gross; A D Moscioni; M Kram; I M Arias; J Roy Chowdhury
Journal:  J Clin Invest       Date:  1987-02       Impact factor: 14.808
  5 in total

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