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Literature DB >> 537910

A tRNA gene of Xenopus laevis contains at least two sites promoting transcription.

A Kressmann, H Hofstetter, E Di Capua, R Grosschedl, M L Birnstiel.

Abstract

A small cloned DNA segment previously shown to contain all genetic information for the expression of the tRNA1met gene of Xenopus laevis was cleaved into an anterior and posterior portion by Hae III restriction. Both restriction fragments were cloned in pCR1 using EcoRI linkers. Starting from these tDNA subclones, a series of new recombinants were constructed. The transcriptional activity of the cloned DNA's was tested both in an in vitro transcriptional system and by means of the oocyte injection technique. It was shown that both the 5' and 3' ends of the cloned gene unit were essential for transcription. We have developed a model for the functional organization of the tRNA1met gene. We propose that the gene contains a regulatory site situated near the 3' portion of the gene unit. For transcription to occur both in vivo and in vitro a specific initiation site near the 5' end of the gene is required.

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Year: 1979 PMID： 537910 PMCID： PMC342346 DOI： 10.1093/nar/7.7.1749

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

13 in total

1. Promoter mutations in the transfer RNA gene tyrT of Escherichia coli.

Authors: M L Berman; A Landy
Journal: Proc Natl Acad Sci U S A Date: 1979-09 Impact factor: 11.205

2. Integration of eukaryotic genes for 5S RNA and histone proteins into a phage lambda receptor.

Authors: S G Clarkson; H O Smith; W Schaffner; K W Gross; M L Birnstiel
Journal: Nucleic Acids Res Date: 1976-10 Impact factor: 16.971

3. A new method for sequencing DNA.

Authors: A M Maxam; W Gilbert
Journal: Proc Natl Acad Sci U S A Date: 1977-02 Impact factor: 11.205

4. A computer aided oligonucleotide analysis provides a model sequence for RNA polymerase-promoter recognition in E.coli.

Authors: G E Scherer; M D Walkinshaw; S Arnott
Journal: Nucleic Acids Res Date: 1978-10 Impact factor: 16.971

5. Nucleotide sequence of a mutant eukaryotic gene: the yeast tyrosine-inserting ochre suppressor SUP4-o.

Authors: H M Goodman; M V Olson; B D Hall
Journal: Proc Natl Acad Sci U S A Date: 1977-12 Impact factor: 11.205

6. Transcription of cloned Xenopus 5S RNA genes by X. laevis RNA polymerase III in reconstituted systems.

Authors: S Y Ng; C S Parker; R G Roeder
Journal: Proc Natl Acad Sci U S A Date: 1979-01 Impact factor: 11.205

7. Delimitation of a promoter for RNA polymerase III by means of a functional test.

Authors: J L Telford; A Kressmann; R A Koski; R Grosschedl; F Müller; S G Clarkson; M L Birnstiel
Journal: Proc Natl Acad Sci U S A Date: 1979-06 Impact factor: 11.205

8. Transcription of cloned tRNA gene fragments and subfragments injected into the oocyte nucleus of Xenopus laevis.

Authors: A Kressmann; S G Clarkson; V Pirrotta; M L Birnstiel
Journal: Proc Natl Acad Sci U S A Date: 1978-03 Impact factor: 11.205

9. Sequence organization of a cloned tDNA met fragment from Xenopus laevis.

Authors: S G Clarkson; V Kurer; H O Smith
Journal: Cell Date: 1978-07 Impact factor: 41.582

10. A nuclear extract of Xenopus laevis oocytes that accurately transcribes 5S RNA genes.

Authors: E H Birkenmeier; D D Brown; E Jordan
Journal: Cell Date: 1978-11 Impact factor: 41.582

39 in total

1. Unusual sequence homology at the 5-ends of the developmentally regulated beta A-, beta C-, and gamma-globin genes of the goat.

Authors: J R Haynes; P Rosteck; J B Lingrel
Journal: Proc Natl Acad Sci U S A Date: 1980-12 Impact factor: 11.205

10. An in vitro RNA polymerase III system from S. cerevisiae: effects of deletions and point mutations upon SUP4 gene transcription.

Authors: R A Koski; D S Allison; M Worthington; B D Hall
Journal: Nucleic Acids Res Date: 1982-12-20 Impact factor: 16.971

A tRNA gene of Xenopus laevis contains at least two sites promoting transcription.

1. Promoter mutations in the transfer RNA gene tyrT of Escherichia coli.

2. Integration of eukaryotic genes for 5S RNA and histone proteins into a phage lambda receptor.

3. A new method for sequencing DNA.

4. A computer aided oligonucleotide analysis provides a model sequence for RNA polymerase-promoter recognition in E.coli.

5. Nucleotide sequence of a mutant eukaryotic gene: the yeast tyrosine-inserting ochre suppressor SUP4-o.

6. Transcription of cloned Xenopus 5S RNA genes by X. laevis RNA polymerase III in reconstituted systems.

7. Delimitation of a promoter for RNA polymerase III by means of a functional test.

8. Transcription of cloned tRNA gene fragments and subfragments injected into the oocyte nucleus of Xenopus laevis.

9. Sequence organization of a cloned tDNA met fragment from Xenopus laevis.

10. A nuclear extract of Xenopus laevis oocytes that accurately transcribes 5S RNA genes.

1. Unusual sequence homology at the 5-ends of the developmentally regulated beta A-, beta C-, and gamma-globin genes of the goat.

2. Distinctive sequence organization and functional programming of an Alu repeat promoter.

3. BC1 RNA: transcriptional analysis of a neural cell-specific RNA polymerase III transcript.

4. In vivo decay kinetic parameters of hammerhead ribozymes.

5. Some bacterial tRNA genes are transcribed by eukaryotic RNA polymerase III.

6. Transcription of the beta-like globin genes and pseudogenes of the goat in a cell-free system.

7. Mammalian tRNA genes: nucleotide sequence of rat genes for tRNAAsp, tRNAGly and tRNAGlu.

8. Deletion mapping of sequences essential for in vivo transcription of the iso-1-cytochrome c gene.

9. Distinct replication-independent and -dependent phases of histone gene expression during the Physarum cell cycle.

10. An in vitro RNA polymerase III system from S. cerevisiae: effects of deletions and point mutations upon SUP4 gene transcription.