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Literature DB >> 6772962

A signal sequence is not sufficient to lead beta-galactosidase out of the cytoplasm.

F Moreno, A V Fowler, M Hall, T J Silhavy, I Zabin, M Schwartz.

Abstract

Escherichia coli strains have been constructed in which lacZ, the gene for the cytoplasmic enzyme beta-galactosidase, is fused to lamB, the gene for an outer membrane protein. One such strain produces a beta-galactosidase which remains cytoplasmic even though it possesses the complete signal sequence of the lamB protein precursor at the amino-terminal end.

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Year: 1980 PMID： 6772962 DOI： 10.1038/286356a0

Source DB: PubMed Journal: Nature ISSN： 0028-0836 Impact factor: 49.962

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Cited

37 in total

1. The net charge of the first 18 residues of the mature sequence affects protein translocation across the cytoplasmic membrane of gram-negative bacteria.

Authors: A V Kajava; S N Zolov; A E Kalinin; M A Nesmeyanova
Journal: J Bacteriol Date: 2000-04 Impact factor: 3.490

Review 2. Export and assembly of bacterial outer membrane proteins.

Authors: J Tommassen; M Struyvé; H de Cock
Journal: Antonie Van Leeuwenhoek Date: 1992-02 Impact factor: 2.271

3. Conformational and membrane-binding properties of a signal sequence are largely unaltered by its adjacent mature region.

Authors: C J McKnight; S J Stradley; J D Jones; L M Gierasch
Journal: Proc Natl Acad Sci U S A Date: 1991-07-01 Impact factor: 11.205

A signal sequence is not sufficient to lead beta-galactosidase out of the cytoplasm.

1. The net charge of the first 18 residues of the mature sequence affects protein translocation across the cytoplasmic membrane of gram-negative bacteria.

Review 2. Export and assembly of bacterial outer membrane proteins.

3. Conformational and membrane-binding properties of a signal sequence are largely unaltered by its adjacent mature region.

4. Regulation of major outer membrane porin proteins of Escherichia coli K 12 by pH.

5. The MURFI linker for multiple reading frame insertion of a sense or nonsense codon into DNA.

6. Folding LacZ in the periplasm of Escherichia coli.

7. Nucleotide sequence of the enterotoxin B gene from Staphylococcus aureus.

8. Secretion of interleukin-1 beta and Escherichia coli galactokinase by Streptomyces lividans.

Review 9. Sequence information required for protein translocation from the cytoplasm.

10. Lambda placMu: a transposable derivative of bacteriophage lambda for creating lacZ protein fusions in a single step.