Literature DB >> 3137209

Secretion of interleukin-1 beta and Escherichia coli galactokinase by Streptomyces lividans.

H Lichenstein1, M E Brawner, L M Miles, C A Meyers, P R Young, P L Simon, T Eckhardt.   

Abstract

The functionality of the Streptomyces lividans beta-galactosidase signal peptide to direct heterologous protein export was examined. The signal peptide plus eight amino acids of mature protein were sufficient to export not only a naturally exported protein, interleukin-1 beta, but also a naturally occurring cytoplasmic protein, Escherichia coli galactokinase. Interestingly, cells which expressed yet exported galactokinase were phenotypically Gal-. The potential use of the exported galactokinase system for the isolation and characterization of mutations within signal peptides and the export machinery of the host is discussed.

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Year:  1988        PMID： 3137209      PMCID： PMC211391          DOI： 10.1128/jb.170.9.3924-3929.1988

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  34 in total

1.  Characterization of the promoter, signal sequence, and amino terminus of a secreted beta-galactosidase from "Streptomyces lividans".

Authors:  T Eckhardt; J Strickler; L Gorniak; W V Burnett; L R Fare
Journal:  J Bacteriol       Date:  1987-09       Impact factor: 3.490

2.  Correlation of competence for export with lack of tertiary structure of the mature species: a study in vivo of maltose-binding protein in E. coli.

Authors:  L L Randall; S J Hardy
Journal:  Cell       Date:  1986-09-12       Impact factor: 41.582

3.  Synthesis of bovine growth hormone by Streptomyces lividans.

Authors:  G Gray; G Selzer; G Buell; P Shaw; S Escanez; S Hofer; P Voegeli; C J Thompson
Journal:  Gene       Date:  1984-12       Impact factor: 3.688

4.  Signal sequences. The limits of variation.

Authors:  G von Heijne
Journal:  J Mol Biol       Date:  1985-07-05       Impact factor: 5.469

5.  Molecular cloning and characterization of the Streptomyces hygroscopicus alpha-amylase gene.

Authors:  S Hoshiko; O Makabe; C Nojiri; K Katsumata; E Satoh; K Nagaoka
Journal:  J Bacteriol       Date:  1987-03       Impact factor: 3.490

6.  Primary structure of the Streptomyces enzyme endo-beta-N-acetylglucosaminidase H.

Authors:  P W Robbins; R B Trimble; D F Wirth; C Hering; F Maley; G F Maley; R Das; B W Gibson; N Royal; K Biemann
Journal:  J Biol Chem       Date:  1984-06-25       Impact factor: 5.157

7.  Purification and characterization of human recombinant interleukin-1 beta.

Authors:  C A Meyers; K O Johanson; L M Miles; P J McDevitt; P L Simon; R L Webb; M J Chen; B P Holskin; J S Lillquist; P R Young
Journal:  J Biol Chem       Date:  1987-08-15       Impact factor: 5.157

8.  Optimization of gene expression in Streptomyces lividans by a transcription terminator.

Authors:  D Pulido; A Jiménez
Journal:  Nucleic Acids Res       Date:  1987-05-26       Impact factor: 16.971

9.  A modified assay for interleukin-1 (IL-1).

Authors:  P L Simon; J T Laydon; J C Lee
Journal:  J Immunol Methods       Date:  1985-11-28       Impact factor: 2.303

10.  Characterization of Streptomyces promoter sequences using the Escherichia coli galactokinase gene.

Authors:  M E Brawner; J I Auerbach; J A Fornwald; M Rosenberg; D P Taylor
Journal:  Gene       Date:  1985       Impact factor: 3.688
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  8 in total

1.  Extracellular production system of heterologous peptide driven by a secretory protease inhibitor of Streptomyces.

Authors:  S Taguchi; M Maeno; H Momose
Journal:  Appl Microbiol Biotechnol       Date:  1992-03       Impact factor: 4.813

2.  Codon adjustment to maximise heterologous gene expression in Streptomyces lividans can lead to decreased mRNA stability and protein yield.

Authors:  E Lammertyn; L Van Mellaert; A P Bijnens; B Joris; J Anné
Journal:  Mol Gen Genet       Date:  1996-02-05

3.  Heterologous expression and secretion of a Streptomyces scabies esterase in Streptomyces lividans and Escherichia coli.

Authors:  V Hale; M McGrew; B Carlson; J L Schottel
Journal:  J Bacteriol       Date:  1992-04       Impact factor: 3.490

4.  Transcriptional analysis of the DD-peptidase/penicillin-binding protein-encoding dac gene of Streptomyces R61: use of the promoter and signal sequences in a secretion vector.

Authors:  C Piron-Fraipont; M V Lenzini; J Dusart; J M Ghuysen
Journal:  Mol Gen Genet       Date:  1990-08

5.  Phospholipase D from Streptomyces antibioticus: cloning, sequencing, expression, and relationship to other phospholipases.

Authors:  Y Iwasaki; H Nakano; T Yamane
Journal:  Appl Microbiol Biotechnol       Date:  1994-11       Impact factor: 4.813

6.  The effect of signal sequences on the efficiency of secretion of a heterologous phosphotriesterase by Streptomyces lividans.

Authors:  S S Rowland; J J Zulty; M Sathyamoorthy; B M Pogell; M K Speedie
Journal:  Appl Microbiol Biotechnol       Date:  1992-10       Impact factor: 4.813

7.  Promoter constructions for efficient secretion expression in Streptomyces lividans.

Authors:  T Schmitt-John; J W Engels
Journal:  Appl Microbiol Biotechnol       Date:  1992-01       Impact factor: 4.813

8.  Synthesis and secretion of hirudin by Streptomyces lividans.

Authors:  E Bender; R Vogel; K P Koller; J Engels
Journal:  Appl Microbiol Biotechnol       Date:  1990-11       Impact factor: 4.813
  8 in total

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