Literature DB >> 6772340

Kinetic studies of a quantitative single-tube enzyme-linked immunosorbent assay.

V C Tsang, B C Wilson, S E Maddison.   

Abstract

We describe a single-cuvette enzyme-linked immunosorbent assay (ELISA) based on enzymic rate kinetics. This kinetic assay can yield linear quantitative data on immunoglobin concentrations. Optimum assay conditions, component concentrations, reaction intervals, and pH are described. Assay linearity and sensitivity are demonstrated in systems involving immunoglobulin G and antibodies to it, and with pooled sera from monkeys infected with Schistosoma mansoni.

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Year:  1980        PMID： 6772340

Source DB:  PubMed          Journal:  Clin Chem        ISSN： 0009-9147            Impact factor:   8.327


  21 in total

1.  Enterotoxigenic Escherichia coli elicits immune responses to multiple surface proteins.

Authors:  Koushik Roy; Scott Bartels; Firdausi Qadri; James M Fleckenstein
Journal:  Infect Immun       Date:  2010-05-10       Impact factor: 3.441

2.  Applying a kinetic method to an indirect ELISA measuring Ostertagia ostertagi antibodies in milk.

Authors:  Raphaël Vanderstichel; Ian Dohoo; Fred Markham
Journal:  Can J Vet Res       Date:  2015-07       Impact factor: 1.310

3.  The kinetics-based enzyme-linked immunosorbent assay for coronavirus antibodies in cats: calibration to the indirect immunofluorescence assay and computerized standardization of results through normalization to control values.

Authors:  J E Barlough; R H Jacobson; D R Downing; T J Lynch; F W Scott
Journal:  Can J Vet Res       Date:  1987-01       Impact factor: 1.310

4.  EatA, an immunogenic protective antigen of enterotoxigenic Escherichia coli, degrades intestinal mucin.

Authors:  Pardeep Kumar; Qingwei Luo; Tim J Vickers; Alaullah Sheikh; Warren G Lewis; James M Fleckenstein
Journal:  Infect Immun       Date:  2013-11-11       Impact factor: 3.441

5.  Effects of dexamethasone on selected parameters of the bovine immune system.

Authors:  J H Pruett; W F Fisher; J R DeLoach
Journal:  Vet Res Commun       Date:  1987       Impact factor: 2.459

6.  Kinetic-dependent enzyme-linked immunosorbent assay for detection of antibodies to Legionella pneumophila.

Authors:  J S Sampson; H W Wilkinson; V C Tsang; B J Brake
Journal:  J Clin Microbiol       Date:  1983-12       Impact factor: 5.948

7.  Enzymatic-based cytometry, a sensitive single-cell cytometric method to assess BCR-ABL1 activity in CML.

Authors:  Jinzhu Yu; Ki Oh; Sitapriya Moorthi; Ling Li; Helmut H Strey; Michael Schuster; Chiara Luberto; Phenix-Lan Quan; Eric Brouzes
Journal:  Lab Chip       Date:  2020-03-03       Impact factor: 6.799

8.  Microvolume, kinetic-dependent enzyme-linked immunosorbent assay for amoeba antibodies.

Authors:  H M Mathews; K W Walls; A Y Huong
Journal:  J Clin Microbiol       Date:  1984-02       Impact factor: 5.948

9.  Protective immunity to Brucella ovis in BALB/c mice following recovery from primary infection or immunization with subcellular vaccines.

Authors:  M P Jiménez de Bagüés; P H Elzer; J M Blasco; C M Marín; C Gamazo; A J Winter
Journal:  Infect Immun       Date:  1994-02       Impact factor: 3.441

10.  Vaccination with Brucella abortus rough mutant RB51 protects BALB/c mice against virulent strains of Brucella abortus, Brucella melitensis, and Brucella ovis.

Authors:  M P Jiménez de Bagüés; P H Elzer; S M Jones; J M Blasco; F M Enright; G G Schurig; A J Winter
Journal:  Infect Immun       Date:  1994-11       Impact factor: 3.441
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