Literature DB >> 32031548

Enzymatic-based cytometry, a sensitive single-cell cytometric method to assess BCR-ABL1 activity in CML.

Jinzhu Yu1, Ki Oh1, Sitapriya Moorthi2, Ling Li1, Helmut H Strey3, Michael Schuster4, Chiara Luberto2, Phenix-Lan Quan1, Eric Brouzes5.   

Abstract

We developed a simple, rapid and cost-effective enzymatic-based cytometry platform to measure intracellular signaling pathway activity. Our single-cell microwell array platform quantifies protein phosphorylation using enzymatic signal amplification and exploiting Michaelis-Menten kinetics. Our method provides a two-fold increase in resolution compared to conventional flow cytometry.

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Year:  2020        PMID： 32031548      PMCID： PMC7439560          DOI： 10.1039/c9lc01213c

Source DB:  PubMed          Journal:  Lab Chip        ISSN： 1473-0189            Impact factor:   6.799


  28 in total

Review 1.  The impact of the new fish technologies on the cytogenetics of haematological malignancies.

Authors:  L Kearney
Journal:  Br J Haematol       Date:  1999-03       Impact factor: 6.998

2.  Assessment of BCR-ABL1 transcript levels at 3 months is the only requirement for predicting outcome for patients with chronic myeloid leukemia treated with tyrosine kinase inhibitors.

Authors:  David Marin; Amr R Ibrahim; Claire Lucas; Gareth Gerrard; Lihui Wang; Richard M Szydlo; Richard E Clark; Jane F Apperley; Dragana Milojkovic; Marco Bua; Jiri Pavlu; Christos Paliompeis; Alistair Reid; Katayoun Rezvani; John M Goldman; Letizia Foroni
Journal:  J Clin Oncol       Date:  2011-11-07       Impact factor: 44.544

3.  Large-scale single-cell trapping and imaging using microwell arrays.

Authors:  Jacqueline R Rettig; Albert Folch
Journal:  Anal Chem       Date:  2005-09-01       Impact factor: 6.986

Review 4.  Mechanisms of BCR-ABL in the pathogenesis of chronic myelogenous leukaemia.

Authors:  Ruibao Ren
Journal:  Nat Rev Cancer       Date:  2005-03       Impact factor: 60.716

5.  BCR-ABL1 tyrosine kinase activity at diagnosis, as determined via the pCrkL/CrkL ratio, is predictive of clinical outcome in chronic myeloid leukaemia.

Authors:  Claire M Lucas; Robert J Harris; Athina Giannoudis; Katy Knight; Sarah J Watmough; Richard E Clark
Journal:  Br J Haematol       Date:  2010-01-11       Impact factor: 6.998

6.  Large-scale investigation of the olfactory receptor space using a microfluidic microwell array.

Authors:  Xavier A Figueroa; Gregory A Cooksey; Scott V Votaw; Lisa F Horowitz; Albert Folch
Journal:  Lab Chip       Date:  2010-02-10       Impact factor: 6.799

7.  A single-molecule digital enzyme assay using alkaline phosphatase with a cumarin-based fluorogenic substrate.

Authors:  Yusuke Obayashi; Ryota Iino; Hiroyuki Noji
Journal:  Analyst       Date:  2015-08-07       Impact factor: 4.616

8.  Detection in primary chronic myeloid leukaemia cells of p210BCR-ABL1 in complexes with adaptor proteins CBL, CRKL, and GRB2.

Authors:  Hetal Patel; Stephen B Marley; Myrtle Y Gordon
Journal:  Genes Chromosomes Cancer       Date:  2006-12       Impact factor: 5.006

9.  Single-cell proteomic analysis of S. cerevisiae reveals the architecture of biological noise.

Authors:  John R S Newman; Sina Ghaemmaghami; Jan Ihmels; David K Breslow; Matthew Noble; Joseph L DeRisi; Jonathan S Weissman
Journal:  Nature       Date:  2006-05-14       Impact factor: 49.962

10.  Single-cell analysis of K562 cells: an imatinib-resistant subpopulation is adherent and has upregulated expression of BCR-ABL mRNA and protein.

Authors:  Ehsan Ghayoor Karimiani; Fiona Marriage; Anita J Merritt; John Burthem; Richard John Byers; Philip J R Day
Journal:  Exp Hematol       Date:  2013-11-20       Impact factor: 3.084
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