Literature DB >> 6772150

Partial purification and characterization of a new intracellular beta-glucosidase of Trichoderma reesei.

M Inglin, B A Feinberg, J R Loewenberg.   

Abstract

A new intracellular beta-glucosidase was isolated from Trichoderma reesei. It was sequentially purified by (NH4)2SO4 precipitation and chromatography and rechromatography on Sephadex G-150. The enzyme has a mol.wt. of 98 000, optimal activity at pH 6.5, pI 4.4 and Km values of 6.7 mM and 3.3 mM for sophorose and cellobiose respectively. Possible functions of the enzyme may be regulation of cellulase induction and/or to serve as a proenzyme.

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Year:  1980        PMID: 6772150      PMCID: PMC1161380          DOI: 10.1042/bj1850515

Source DB:  PubMed          Journal:  Biochem J        ISSN: 0264-6021            Impact factor:   3.857


  8 in total

1.  Induction of cellulase in fungi by cellobiose.

Authors:  M MANDELS; E T REESE
Journal:  J Bacteriol       Date:  1960-06       Impact factor: 3.490

2.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

3.  The mechanism of enzymatic cellulose degradation. Isolation and some properties of a beta-glucosidase from Trichoderma viride.

Authors:  L E Berghem; L G Pettersson
Journal:  Eur J Biochem       Date:  1974-07-15

4.  Catabolite repression of cellulase formation in Trichoderma viride.

Authors:  T Nisizawa; H Suzuki; K Nisizawa
Journal:  J Biochem       Date:  1972-06       Impact factor: 3.387

5.  Cellobiase from Trichoderma viride: purification, properties, kinetics, and mechanism.

Authors:  C S Gong; M R Ladisch; G T Tsao
Journal:  Biotechnol Bioeng       Date:  1977-07       Impact factor: 4.530

6.  Induction of cellulolytic enzymes in Trichoderma reesei by sophorose.

Authors:  D Sternberg; G R Mandels
Journal:  J Bacteriol       Date:  1979-09       Impact factor: 3.490

7.  Sophorose metabolism and cellulase induction in Trichoderma.

Authors:  J R Loewenberg; C M Chapman
Journal:  Arch Microbiol       Date:  1977-05-13       Impact factor: 2.552

8.  Sophorose as an inducer of cellulase in Trichoderma viride.

Authors:  M MANDELS; F W PARRISH; E T REESE
Journal:  J Bacteriol       Date:  1962-02       Impact factor: 3.490

  8 in total
  14 in total

Review 1.  Microbial cellulose utilization: fundamentals and biotechnology.

Authors:  Lee R Lynd; Paul J Weimer; Willem H van Zyl; Isak S Pretorius
Journal:  Microbiol Mol Biol Rev       Date:  2002-09       Impact factor: 11.056

2.  Purification and Properties of beta-Glucosidase from Aspergillus terreus.

Authors:  W E Workman; D F Day
Journal:  Appl Environ Microbiol       Date:  1982-12       Impact factor: 4.792

3.  Separation and Some Properties of Two Intracellular beta-Glucosidases of Sporotrichum (Chrysosporium) thermophile.

Authors:  H P Meyer; G Canevascini
Journal:  Appl Environ Microbiol       Date:  1981-04       Impact factor: 4.792

4.  Comparison of beta-Glucosidase Activities in Different Streptomyces Strains.

Authors:  N Moldoveanu; D Kluepfel
Journal:  Appl Environ Microbiol       Date:  1983-07       Impact factor: 4.792

5.  Expression and characterization of a cold-active and xylose-stimulated β-glucosidase from Marinomonas MWYL1 in Escherichia coli.

Authors:  Wei Zhao; Rihe Peng; Aisheng Xiong; Xiaoyan Fu; Yongsheng Tian; Quanhong Yao
Journal:  Mol Biol Rep       Date:  2011-06-18       Impact factor: 2.316

6.  Sophorose induction of an intracellular b-glucosidase in Trichoderma.

Authors:  J R Loewenberg
Journal:  Arch Microbiol       Date:  1984-01       Impact factor: 2.552

7.  Purification and properties of a stable beta-glucosidase from an extremely thermophilic anaerobic bacterium.

Authors:  M L Patchett; R M Daniel; H W Morgan
Journal:  Biochem J       Date:  1987-05-01       Impact factor: 3.857

8.  Enzymatic properties and intracellular localization of the novel Trichoderma reesei beta-glucosidase BGLII (cel1A).

Authors:  Markku Saloheimo; Juha Kuja-Panula; Erkko Ylösmäki; Michael Ward; Merja Penttilä
Journal:  Appl Environ Microbiol       Date:  2002-09       Impact factor: 4.792

9.  Differential involvement of β-glucosidases from Hypocrea jecorina in rapid induction of cellulase genes by cellulose and cellobiose.

Authors:  Qingxin Zhou; Jintao Xu; Yanbo Kou; Xinxing Lv; Xi Zhang; Guolei Zhao; Weixin Zhang; Guanjun Chen; Weifeng Liu
Journal:  Eukaryot Cell       Date:  2012-09-21

10.  Metabolic engineering strategies for the improvement of cellulase production by Hypocrea jecorina.

Authors:  Christian P Kubicek; Marianna Mikus; André Schuster; Monika Schmoll; Bernhard Seiboth
Journal:  Biotechnol Biofuels       Date:  2009-09-01       Impact factor: 6.040

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